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Fast, green and high-efficiency extraction method of silkworm excrement flavone and detection method thereof

A flavonoid and green technology, which is applied in the field of rapid, green and efficient extraction of flavonoids from silkworm flavonoids, can solve problems such as being unsuitable for large-scale production requirements, cumbersome steps, low safety, etc., and achieves avoiding environmental pollution and food safety problems, the process is simple, and the filtering process is simple. Difficulty reduction effect

Active Publication Date: 2019-09-13
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction method of flavonoids generally adopts organic solvent extraction. Although the extraction rate of flavonoids is high, the safety is low and the steps are cumbersome, which is not suitable for large-scale production requirements.

Method used

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  • Fast, green and high-efficiency extraction method of silkworm excrement flavone and detection method thereof
  • Fast, green and high-efficiency extraction method of silkworm excrement flavone and detection method thereof
  • Fast, green and high-efficiency extraction method of silkworm excrement flavone and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for fast, green and efficient extraction of silk flavonoids, comprising the following steps:

[0029] (1) Crush the dried silkworm excrement and pass through a 50-mesh sieve;

[0030] (2) Take 120g of dried and crushed silkworm excrement powder, add 2400mL of ultrapure water at a water temperature of 45°C (the ratio of solid to liquid is 1g:20mL), and soak for 30 minutes. Put it into ultrasonic extraction for 70min, pour the filter residue and filtrate into the flash extractor for standby;

[0031] (3) Set up the flash extractor, turn on the switch, raise the knife head, place the solution in step (1) on the operating table, lower the knife head and place the knife head in the liquid surface 1cm away from the bottom of the beaker (the knife head should not be in contact with the bottom of the beaker) touch);

[0032] (4) Be careful not to adjust the gear suddenly in advance of the flash, and adjust the gear slowly to the required gear after the cutter head ro...

Embodiment 2

[0037] A method for fast, green and efficient extraction of silk flavonoids, comprising the following steps:

[0038]According to the steps of Example 1, only the dosage ratio of the pulverized silkworm excrement obtained in step (1) in step (2) and the aqueous solution with a water temperature of 45°C is replaced from 1g:20mL to 1g:25mL, 1g:30mL, 1g :35mL, 1g:40mL, the content of gained silk flavonoids is as follows figure 1 As shown, it is 3.54mg / g, 4.48mg / g, 4.08mg / g, 4.34mg / g in turn.

Embodiment 3

[0040] A method for fast, green and efficient extraction of silk flavonoids, comprising the following steps:

[0041] According to the steps of Example 1, only the ultrasonic extraction time in step (2) is replaced by 70min to 40min, 50min, 60min and 80min, the content of the obtained silk flavonoids is as follows figure 2 As shown, it is 4.00mg / g, 4.08mg / g, 4.28mg / g, 3.38mg / g in turn.

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Abstract

The invention relates to extraction of effective constituents of traditional Chinese medicine, and particularly discloses a fast, green and high-efficiency extraction method of silkworm excrement flavone and a detection method thereof. The extraction method comprises the following steps: collecting silkworm excrement and removing impurities, and obtaining silkworm excrement powder after drying andsmashing the silkworm excrement; by a method of combination of assistance of ultrasonic wave and flash extraction, extracting the silkworm excrement powder with water solution, concentrating and drying extracting solution, and determining content of flavone. In the extraction method provided by the invention, by the method of combination of ultrasonic extraction and flash extraction, compared with the conventional extraction method, damage to thermolabile constituents is avoided, and the extraction method provided by the invention has high yield, is green and environmental-friendly, and remarkably improves transfer rate of effective constituents. Compared with the optimized flavone matter ultrasonic extraction method, the extraction method provided by the invention improves content of flavone by 19.2%, extraction conditions are moderate, usage of all organic solvent is avoided, extractant is environmental-friendly, and the extraction method provided by the invention has the characteristics, such as low extraction cost, and so on; moreover, the waste silkworm excrement is reused, thus, the waste is turned into wealth, and a problem of environment pollution is solved greatly.

Description

technical field [0001] The invention relates to the extraction of active ingredients of traditional Chinese medicines, in particular to a method for rapidly, green and high-efficiency extraction of silk flavonoids and a detection method thereof. Background technique [0002] Silkworm excrement, also known as silkworm excrement, is the feces excreted by the larvae of the silkworm moth, Bombyx mori, and is a commonly used traditional Chinese medicinal material in clinical practice. Sweet and warm in nature, it can dispel wind and dampness, promote blood circulation, relieve pain, and sterilize. It is often used to treat rheumatic arthralgia, numbness, skin eczema itching, cholera, and abdominal pain. Studies have shown that silkworm excrement contains flavonoids, volatile oils, steroids, phenols and terpenes. my country's silkworm excrement resources are very rich, but most of them have not been effectively utilized. The full development and utilization of silkworm excrement ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/33G01N1/28G01N1/30G01N1/34
CPCG01N1/28G01N1/286G01N1/30G01N1/34G01N21/31G01N21/33G01N2021/3196
Inventor 时桂芹任菲沈佳鑫侯梦丹
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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