A kind of method for controlling Bemisia tabaci by cooperating with Cordyceps javanica

A technology for the bemiphid wasp and bemisia tabaci, applied in the field of biological control, can solve the problems of Q-type bemisia tabaci hazard aggravation, environmental factors, and unstable control effect, and achieve easy operation and popularization, and improve Efficiency of dispersal, conservation of biodiversity and effects of natural enemy resources

Active Publication Date: 2021-10-29
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2003, the cryptic species of Bemisia tabaci MEAM1 (type Q bemisia) was discovered for the first time to invade my country, and the damage of type Q bemisia gradually increased in some countries and regions. Powder wind has become the main endangered species, and it is spreading further, causing more serious losses
However, there are still many problems in the control effect of these bio-control bacteria and natural enemies, which need to be better improved, such as the existence of single control factors, greater influence by environmental factors, unstable control effects, environmental safety and biodiversity. sexual issues

Method used

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  • A kind of method for controlling Bemisia tabaci by cooperating with Cordyceps javanica
  • A kind of method for controlling Bemisia tabaci by cooperating with Cordyceps javanica
  • A kind of method for controlling Bemisia tabaci by cooperating with Cordyceps javanica

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Analysis of the pathogenicity of the Cordyceps javanica GZQ-1 strain to the wasp

[0049] 1. The pathogenicity experiment of Cordyceps japonica on the pupae of Aphid spp.

[0050] (1) Experimental method

[0051] Cotton seedlings with the same growth status were selected, and 60 pairs of adult whitefly were inserted into each box by using the inoculation box, and the adult whitefly were removed after 24 hours. When Bemisia tabaci developed to the second instar, it was inserted into the wasp of Aphididae, and it was used in the experiment when the wasp developed to the pupal stage.

[0052] Using the soaking method, the cotton leaves with the pupae of Aphid spp. were immersed in the spore suspension of Cordyceps javanica for 15s (1×10 3 Spores / mL, 1×10 4 Spores / mL, 1×10 5 Spores / mL, 1×10 6 Spores / mL, 1×10 7 Spores / mL, 1×10 8 spores / mL), and 1% Tween-80 was used as a control. Put the cotton leaves in agar (10g.L -1 ) in a petri dish, sealed with perfora...

Embodiment 2

[0064] Example 2 Effects of Cordyceps Javanese GZQ-1 Strain on the Parasitic Potential of the Aphid spp.

[0065] 1. Non-selective experiment (single age)

[0066] (1) Experimental method

[0067] Cotton seedlings with the same growth conditions were selected, put on the inoculation boxes, and 40 pairs of adult whitefly were inserted into each box, and the adults of whitefly were removed after 24 hours of oviposition. Each treated Bemisia tabaci was used in the experiment when it developed to the corresponding age (1-4). Java Cordyceps spore suspension (1 × 10 8 spores / mL) were evenly sprayed onto the leaves with nymphs of various ages until there were droplets dripping down, and after drying naturally, a box with a larger diameter was selected to clamp the whitefly nymphs and connected to the paddles that had just emerged. Horned aphid wasps, remove the parasitic wasps after 24 hours. Tween-80 was used as a control experiment, and each treatment was repeated 5 times. Obs...

Embodiment 3

[0079] Example 3 Cordyceps javanica GZQ-1 strain combined with Aphidia spp. to control Bemisia tabaci

[0080] 1. Experimental method

[0081] (1) Breeding of the tested population of whitefly: select healthy cotton seedlings with the same growth, insert the adults of whitefly by using the miniature insect box, after 24 hours of laying eggs, remove the adults of whitefly, and put the cotton seedlings connected with tobacco powder The cotton seedlings of the lice are put into insect cages, raised at a temperature of 26±1° C. and a humidity of 60 to 80%, and regularly fertilized and watered;

[0082] (2) Breeding of the tested population of Aphididae wasps: select cotton seedling plants with 2nd to 3rd instar nymphs of Bemisia tabaci, use a small finger-shaped tube to absorb Aphididae wasps, and inoculate them for 24 hours through a miniature inoculation box. Afterwards, it was removed and used for experiments after 3 to 5 generations of breeding;

[0083] (3) Cultivation of t...

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Abstract

The invention discloses a method for controlling Bemisia tabaci by cooperating with Cordyceps javanica. The present invention firstly provides the application of the Aphidia spp. and Cordyceps javanica in preventing and controlling whitefly pests, preparing whitefly pest control medicines and preventing and controlling diseases caused by whitefly pests. Based on the above application, the present invention provides a method for controlling Bemisia tabaci in cooperation with Cordyceps javanica, by spraying the suspension of Cordyceps javanica spores and releasing the wasps of A. The method is simple, easy to operate and popularize. In the prevention and control of whitefly pests, it can reduce the use of chemical pesticides or even avoid the use of chemical pesticides, reduce the damage rate of crops, vegetables or flowers, and protect biodiversity and natural enemy resources. Improve the transmission efficiency of bio-control microorganisms, and open up an important way for the integrated control technology system of whitefly pests.

Description

technical field [0001] The invention belongs to the technical field of biological control, and more specifically relates to a method for controlling Bemisia tabaci by coordinating with Cordyceps javanica. Background technique [0002] Whitefly pests are a kind of pests that are often encountered in agricultural production, and whitefly is a very characteristic representative species of this kind of pests. Bemisia tabaci (Gennadius) belongs to Hemiptera (Hemiptera) Aleyrodidae. It is a kind of highly omnivorous insect. It was first discovered by Gennadius on tobacco in Greece in 1889. Bemisia tabaci is a complex of several cryptic species, and 15 cryptic species including 13 native species and 2 global invasive species have been reported in my country. As an important economic pest, Bemisia tabaci is one of the main pests of tropical and subtropical protected areas and field crops. It is very easy to break out and cause disasters after invasion, and it is difficult to stop i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G13/00A01K67/033
CPCA01G13/00A01K67/033
Inventor 邱宝利欧达阿里·肖卡特任丽梅
Owner SOUTH CHINA AGRI UNIV
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