Culture method for improving selenium-enriching effect of lactobacillus rhamnosus
A technique for cultivating Lactobacillus rhamnosus and a culture method, which is applied in the field of cultivating the selenium-enriching effect of Lactobacillus rhamnosus, can solve the problem of low selenium-enriching amount, and achieve the goal of improving enrichment effect, significant effect, and optimizing condition parameters Effect
Active Publication Date: 2019-09-17
陕西英童乳业有限公司
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Problems solved by technology
However, the amount of selenium enrichment is low, so it is extremely important to study methods that can promote the selenium enrichment effect of lactic acid bacteria
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[0032] The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.
[0033] The present invention comprises the following steps:
[0034] Step 1, Activation of Strains
[0035] Inject 0.3-0.5mL of MRS broth medium sterilized by high temperature and high pressure into the freeze-drying tube, and gently blow it with a pipette gun to fully dissolve it into a bacterial suspension. Aspirate the bacterial suspension, put them all into 2 test tubes, and cultivate them under the specified conditions of the strains. After the activation is successful, in order to improve the activity of the strains, inoculate each strain into the MRS broth medium, and place them in a constant temperature incubator. Cultivate at 37°C for 24-48 hours, activate three generations continuously, and obtain activated bacterial liquid.
[0036] Step 2, CaCl 2 pretreatment activated bacteria...
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The invention provides a culture method for improving the selenium-enriching effect of lactobacillus rhamnosus. The method comprises the steps that 1, the lyophilized lactobacillus rhamnosus is cultured and activated, and activated bacterial liquid is obtained; 2, the activated bacterial liquid is separated, thallus is collected and treated with a CaCl2 solution, then resuscitation culture is conducted, and a test bacterial solution is obtained; 3, the test bacterial liquid is inoculated into a culture medium, culture is conducted to the log phase, then heat stress treatment is conducted at 45-55 DEG C, after heat stress is completed, a sodium selenite solution is added in the culture medium, and selenium-enriched culture is conducted. Accordingly, the lactobacillus rhamnosus serves as a study object, pretreatment is conducted with CaCl2, the bacterium is subjected to heat stress, after heat stress is conducted, selenium-enriched culture is conducted, and the selenium-enriching effect of the lactobacillus rhamnosus is obviously improved.
Description
technical field [0001] The invention belongs to the field of biology, and specifically discloses a cultivation method for improving the selenium-enriching effect of Lactobacillus rhamnosus. Background technique [0002] Selenium is an essential trace element for the human body. There are about 35 kinds of selenoproteins found so far. Selenium is a component of thyroxine deiodinase (ID), thioredoxin reductase (TrxR), selenophosphate synthase (SPS) and glutathione The components of enzyme active centers such as peptide peroxidase (GPx) have the physiological functions of protecting the structure and function of human cell membranes from excessive oxidative damage, anti-cancer, detoxification, liver protection and improving human immunity. Organic selenium is less toxic than inorganic selenium, and has higher biological activity, which can be effectively assimilated in the human body and is conducive to absorption in the body. Therefore, the artificial supplementation of organ...
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Login to View More IPC IPC(8): C12N1/38C12N1/36C12N1/20C12R1/225
CPCC12N1/20C12N1/36C12N1/38
Inventor 徐颖贺佳璇李国梁李恬王盼雪邬淑芳
Owner 陕西英童乳业有限公司