Method for culturing anaerobic ammonia oxidation granular sludge resistant to high-concentration spiramycin

Abstract

The invention discloses a method for culturing anaerobic ammonia oxidation granular sludge resistant to high-concentration spiramycin. The method comprises the following steps: adding spiromycin into the influent water of an anaerobic ammonium oxidation reactor and tracing the changes of the effluent water of the reactor, wherein the influent water contains nitrite nitrogen and ammonia nitrogen in a mass concentration ratio of 1: (0.9-1.1); stopping the addition of spiramycin according to the condition of the effluent water of the reactor, proportionally reducing the concentrations of ammonia nitrogen and nitrite nitrogen in the influent water, and restoring the operating performance of the reactor by proportionally and gradiently increasing the concentrations of ammonia nitrogen and nitrite nitrogen in the influent water; and after the reactor performance is restored, adding spiromycin again, wherein the denitrification performance of the reactor is stabilized after fluctuation, and the cultivation of the anaerobic ammonia oxidation granular sludge resistant to high-concentration spiramycinis completed. The method of the invention gradually induces changes in spiramycin resistance genes by the means of primary exposure-gradient restoration-secondary exposure, so anaerobic ammonia oxidation granular sludge resistant to long-term spiramycin interference is cultivated.

Description

technical field [0001] The invention relates to the technical field of sludge cultivation, in particular to a method for cultivating anaerobic ammonium oxidation granular sludge resistant to high concentrations of spiramycin. Background technique [0002] Antibiotics have played an irreplaceable and important role in human medicine, livestock and poultry farming, and aquaculture. my country is a big country in the production and use of antibiotics, but there is also an obvious abuse of antibiotics. The antibiotics used in the aquaculture industry account for about 50% of the antibiotics used in my country every year. The vast majority of antibiotics will not be completely absorbed by the organism after entering the organism, and a large part (>70%) will be excreted into the natural environment with excrement, resulting in a large amount of antibiotics remaining in the antibiotic production wastewater. In my country, different types and concentrations of antibiotics have ...

AI Technical Summary

Problems solved by technology

Sludge maintains low biological activity due to the influence of antibiotics, causing organic matter to produce toxic by-products such as sulfide and ammonia, thereby further reducing the degradation rate of organic matter in the bottom sludge and destroying the microbial community in the soil environment. Change or even prevent the material cycle of the ecosystem, reduce species diversity, interfere with the material cycle and energy flow of the soil ecosystem, and there are ecological and environmental risks that cannot be ignored

[0004] The anaerobic ammonium oxidation process is a green and sustainable denitrification process. It uses nitrite as an electron acceptor to oxidize ammonia into nitrogen. Oxyammox bacteria proliferate slowly, with low cell productivity, and are easily affected by the external environment, while the residual spiramycin in wastewater can inhibit the synthesis of proteins in bacteria, thereby inhibiting the growth of anammox bacteria

[0005] Therefore, when using the anammox process to treat spiramycin wastewater, it often faces risks such as inactivation of anammox bacteria.

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