Phenylacrylamide compounds with antifeedant effect on cotton bollworm in Solanum echinacea and its extraction and separation method and application

A technology of phenylacrylamide and Solanum thorn, applied in the agricultural field, can solve the problem of not paying attention to the chemical defense of herbivorous insects and the like

Active Publication Date: 2022-05-10
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, people have done a lot of research on the invasion mechanism of exotic plants in terms of biology and ecology, but the chemical defense of invasive plants against herbivorous insects is often ignored or ignored

Method used

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  • Phenylacrylamide compounds with antifeedant effect on cotton bollworm in Solanum echinacea and its extraction and separation method and application
  • Phenylacrylamide compounds with antifeedant effect on cotton bollworm in Solanum echinacea and its extraction and separation method and application
  • Phenylacrylamide compounds with antifeedant effect on cotton bollworm in Solanum echinacea and its extraction and separation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1 Extraction and separation of phenylacrylamides that have antifeedant effects on cotton bollworms in Solanum solanum thorns

[0073] 1) Take 15 kg of dry aerial parts of Solanum erythroechii and extract them by cold soaking in 100 L of 70% ethanol for 3 times, each time for 6 days. The combined extracts are concentrated under reduced pressure to obtain 1.2 kg of extract.

[0074] 2) After suspending 1.2 kg of extract extract with 3 L of distilled water, extract with 3.2 L of petroleum ether and 3.2 L of ethyl acetate respectively, and concentrate the extract under reduced pressure to obtain 220 g of extract extract.

[0075] 3) The extraction extract is chromatographed on a silica gel column, eluted multiple times with dichloromethane and methanol systems, and the volume ratios are 100:1, 50:1, 30:1, 20:1, 10:1, 5 :1, 2:1, 1:1, each 500mL is a fraction, a total of 25 fractions were collected, detected by silica gel thin layer chromatography, and combined into f...

Embodiment 2

[0079] Example 2 Extraction and separation of phenylacrylamides that have an antifeedant effect on cotton bollworms in Solanum thorns

[0080] 1) Take 22 kg of dried above-ground parts of Solanum nigrum and use 120 L of 70% ethanol to cold soak and extract 4 times, each time for 7 days, and the combined extracts are concentrated under reduced pressure to obtain 1.4 kg of extract.

[0081] 2) After suspending 1.4 kg of extract extract with 4 L of distilled water, extract with 5.0 L of petroleum ether and 5.0 L of ethyl acetate respectively, and concentrate the extract under reduced pressure to obtain 260 g of extract extract.

[0082] 3) The extraction extract is chromatographed on a silica gel column, eluted multiple times with dichloromethane and methanol systems, and the volume ratios are 100:1, 50:1, 30:1, 20:1, 10:1, 5 :1, 1:1, each 500mL is a fraction, a total of 27 fractions were collected, detected by silica gel thin layer chromatography, and combined into four parts Fr...

Embodiment 3

[0086] Example 3 Extraction and separation of phenylacrylamides that have an antifeedant effect on cotton bollworms in Solanum thorns

[0087] 1) Take 24 kg of dry above-ground parts of Solanum solanum and use 120 L of 75% ethanol to cold soak and extract 3 times, each time for 5 days, and combine the extracts to concentrate under reduced pressure to obtain 1.6 kg of extract.

[0088] 2) After suspending 1.6 kg of extract extract with 5 L of distilled water, extract with 7.5 L of petroleum ether and 7.5 L of ethyl acetate respectively, and concentrate the extract under reduced pressure to obtain 270 g of extract extract.

[0089] 3) The extraction extract is chromatographed on a silica gel column, eluted multiple times with dichloromethane and methanol systems, and the volume ratios are 100:1, 50:1, 20:1, 10:1, 8:1, 5 :1, 2:1, 1:1, each 500mL is a fraction, a total of 25 fractions were collected, detected by silica gel thin layer chromatography, and combined into four parts Fr...

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Abstract

The invention belongs to the technical field of agriculture, and in particular relates to phenylacrylamide compounds. The extraction and separation method is as follows: take dried Solanum solanum and then concentrate under reduced pressure to obtain the extract; after suspending in distilled water, extract with petroleum ether and ethyl acetate respectively, and concentrate under reduced pressure to obtain the extract; Chromatography, eluting, collecting fractions, and silica gel thin-layer chromatography, to obtain four parts Fr.A ~ Fr.D; Fr.B is eluted by silica gel column chromatography, collecting fractions, silica gel thin-layer chromatography, Get Fr.B-1~Fr.B-6 six parts; .B-2-5 five parts; using high performance liquid chromatography, in Fr.B-2-2 ~ Fr.B-2-4 separation to obtain the target product. The extraction and separation method of the invention is simple, has good reproducibility and high extraction purity. The obtained compound has good antifeedant activity to cotton bollworm.

Description

technical field [0001] The invention belongs to the field of agricultural technology, and relates to phenylacrylamide compounds in Solanum nigrum and an extraction and separation method thereof, and also relates to the application of the compounds in inhibiting the feeding activity of cotton bollworms. Background technique [0002] In recent years, the serious ecological and economic problems caused by the invasion of alien plants have attracted much attention. In addition to some basic biological characteristics, the successful invasion of alien plants in new habitats should also have some unique invasion mechanisms. For a long time, people have done a lot of research on the invasion mechanism of exotic plants in terms of biology and ecology, but the chemical defense of invasive plants against herbivorous insects is often ignored or ignored. In fact, during the evolution process of invasive plants and phytophagous insects in the new environment, there is a chemical interac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C233/22C07C231/24A01N37/18A01P7/04A01P17/00
CPCC07C233/22C07C231/24A01N37/18C07B2200/07
Inventor 刘志翔安桐刘策袁瑞
Owner SHENYANG AGRI UNIV
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