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Preparation method of bile acid and its derivative surfactant modified metal organic skeleton nano composite catalyst and application thereof

A metal-organic framework and surfactant technology, applied in biochemical equipment and methods, immobilized on/in organic carriers, enzymes, etc., can solve problems such as limiting the industrial production and application of PLB, and achieve short cycle and simple operation. , the effect of improving stability and activity

Pending Publication Date: 2019-09-20
KAIPING GENUINE BIOCHEM PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, at present, there are few research reports on the immobilization of PLB at home and abroad, which greatly limits the industrial production and application of PLB.

Method used

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  • Preparation method of bile acid and its derivative surfactant modified metal organic skeleton nano composite catalyst and application thereof
  • Preparation method of bile acid and its derivative surfactant modified metal organic skeleton nano composite catalyst and application thereof
  • Preparation method of bile acid and its derivative surfactant modified metal organic skeleton nano composite catalyst and application thereof

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Experimental program
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Effect test

Embodiment 1

[0027] A method for preparing a metal-organic framework nanocomposite catalyst modified by cholic acid and its derivative biosurfactant, comprising the following steps:

[0028] Step 1, Preparation of Metal-Organic Framework Nanocomposite Catalysts

[0029] Prepare 0.5-2.5 mol / L imidazole aqueous solution, at room temperature, add dropwise free enzyme solution while stirring, and add 0.2-0.6 mol / L metal ion salt solution dropwise to the mixed solution while stirring.

[0030] Step 2, cholic acid and its derivatives biosurfactant modified free metal-organic framework nanocomposite catalyst

[0031] Quickly add 10mmol / L-100mmol / L solution of cholic acid and its derivatives to the mixture. Stir at room temperature for 30-60 minutes to obtain an aqueous solution of cholic acid / enzyme / metal-organic framework nanocomposite catalyst; centrifuge, wash with deionized water for 1-3 times, and vacuum freeze-dry to constant weight.

[0032] As an improvement, the surfactant is one of li...

Embodiment 2

[0040] The comparison of the catalytic activities of Free PLB, PLB / ZIF-8 and LCA@PLB / ZIF-8 was studied

[0041] LCA is one of the components of bile acids, which are the end products of cholesterol catabolism in mammals. The formed bile salts are a class of biosurfactants with special structures and unusual properties, which can help Dissolve insoluble substances such as fat and fat-soluble vitamins. Bile salts, as soluble amphiphilic compounds, can be used as absorption enhancers in pharmaceutical formulations, in addition, they can also be used as excipients for enzyme stability. In addition, it can also form a series of nanocomposites with different sizes and structures through metal coordination with divalent metal ions. Such as image 3 The addition of the lithocholic acid modifiers has a good effect on improving the enzyme activity. Compared with the unmodified PLB / ZIF-8, the enzyme activity has increased by about 126%.

Embodiment 3

[0043] The reusability of PLB / ZIF-8 and LCA@PLB / ZIF-8 was investigated

[0044] Although the structure of PLB / ZIF-8 and LCA@PLB / ZIF-8 will suffer from mechanical damage and enzyme inactivation in the process of catalytic reaction and centrifugal washing in the process of recycling, but after repeated use for 10 consecutive After cycling, it was found that the residual activity of PLB / ZIF-8 was still above 70%, while that of LCA@PLB / ZIF-8 was maintained above 80%. The overall cost for industrial use can thus be greatly reduced. However, Free PLB in aqueous solution is difficult to recover and reuse for many times through general separation technology, which increases the cost of industrial use.

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Abstract

The invention discloses a preparation method of a bile acid and its derivative surfactant modified metal organic skeleton nano composite catalyst and an application thereof. The preparation method comprises the following steps: preparing an aqueous solution of imidazole and its derivative, and adding a free enzyme solution while stirring at room temperature to obtain a mixed solution 1; adding a metal ion salt solution drop by drop into the mixture 1 while stirring to obtain a mixed solution 2, then rapidly adding the cholic acid and its derivative solution to the mixture 2 within 1 min, stirring the materials for 30 to 60 minutes, performing centrifugation and rinsing the material with deionized water for 1 to 3 times, and performing vacuum freeze drying to constant weight. The invention also provides the application of the catalyst in biocatalysis. The method adopts an aqueous phase solution mixing method to prepare, has the advantages of simple operation, short cycle, mild condition, low cost, high enzyme stability, high enzyme recovery rate, high binding strength of carrier enzyme molecule, and combination of biological and physical catalytic activity.

Description

technical field [0001] The invention belongs to the technical field of enzyme immobilization, and in particular relates to a preparation method and application of a metal-organic framework nanocomposite catalyst modified by surfactants of cholic acid and its derivatives. Background technique [0002] The enzyme reaction is carried out in aqueous solution, but there are many problems in the aqueous phase system, for example, the free enzyme is relatively unstable, and under the influence of external factors such as high temperature, acid and alkali, the enzyme is volatile and inactivated; when the substrate and the enzyme react completely Finally, even if the enzyme activity is still maintained at a high level, it is difficult to recycle and reuse, and the enzyme is mixed together as impurities and products after the reaction, which not only brings difficulties to the later separation and purification, but also increases the cost of industrial production. cost. Enzyme immobi...

Claims

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Application Information

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IPC IPC(8): C12N11/02C12N9/16C12N9/18C12N9/20C12N9/50C12P13/00
CPCC12N11/02C12N9/20C12Y301/04004C12Y301/04003C12Y301/01003C12N9/50C12N9/18C12N9/16C12P13/001
Inventor 陈可泉卢媛媛李辉王昕欧阳平凯
Owner KAIPING GENUINE BIOCHEM PHARMA