Exopolysaccharide flocculant and preparation method and application thereof
An extracellular polysaccharide and flocculant technology, which is applied to the exopolysaccharide flocculant produced by P. chrysosporium and its preparation field, can solve the problems of not having thermal stability, spatial structure change, influence on flocculation activity, etc. High use value, no secondary pollution, good flocculation effect
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Embodiment 1
[0036] An exopolysaccharide flocculant, the exopolysaccharide flocculant is the exopolysaccharide produced by Phanerochaete chrysosporium, and the exopolysaccharide is mainly composed of glucose; the molecular weight of the exopolysaccharide flocculant is 1.76×10 6 The polysaccharide of Da accounts for 82.31%, and the molecular weight is 3.95×10 7 The polysaccharides of Da accounted for 15.77%, and the molecular weight of the remaining polysaccharides was 1.92×10 8 Da.
[0037] In this example, the exopolysaccharide flocculant is mainly connected by (1,4) glucosidic bonds.
[0038] In this embodiment, the mass content of total sugar in the exopolysaccharide flocculant is 96%-98%.
[0039] A preparation method of the exopolysaccharide flocculant in the above-mentioned present embodiment, comprising the following steps:
[0040] S1. Put Phanerochaete chrysosporium in the culture solution, and cultivate it for 7 days at a temperature of 30° C. and a rotation speed of 180 rpm. ...
Embodiment 2
[0054] The application of the exopolysaccharide flocculant in the treatment of suspended solids in sewage is specifically the use of the exopolysaccharide flocculant prepared in Example 1 to treat kaolin in sewage, including the following steps:
[0055] The exopolysaccharide flocculant prepared in Example 1 of different volumes was added to 200mL kaolin solution (the concentration of kaolin in the solution was 5g / L, and the particle size of kaolin in the solution was 2.5 μm, and the pH value of the solution was 7.0 , the temperature is 20°C), so that the concentration of polysaccharides in the kaolin solution reaches 0.6mg / L, 0.75mg / L, 0.9mg / L, 1.05mg / L, 1.2mg / L, 1.35mg / L, 1.5mg / L , 1.8mg / L, 2mg / L, stirred at 400rpm for 1min, then stirred at 40rpm for 3min, and stood for 5min to complete the treatment of kaolin in sewage.
[0056] After finishing the treatment, take the solution at 6cm below the liquid surface, and measure the absorbance OD of the solution at a wavelength of ...
Embodiment 3
[0062] The application of the exopolysaccharide flocculant in the treatment of suspended solids in sewage is specifically the use of the exopolysaccharide flocculant prepared in Example 1 to treat kaolin in sewage, including the following steps:
[0063] Get the kaolin solution that pH value is respectively 1,2,3,4,5,6,7,8,9,10,11,12 (the concentration of kaolin in this solution is 5g / L, and the particle size of kaolin in this solution Diameter is 2.5 μm, solution temperature is 20 ℃), the volume is 200mL, add the extracellular polysaccharide flocculant that makes in Example 1 respectively, make the polysaccharide concentration in each kaolin solution be 1mg / L, stir 1min at 400rpm, then Stirring at 40rpm for 3 minutes and standing still for 5 minutes completes the treatment of kaolin in sewage.
[0064] After finishing the treatment, take the solution at 6cm below the liquid surface, and measure the absorbance OD of the solution at a wavelength of 550nm 550 . The system with...
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