Separation method of EVs (extracellular vesicles)

A separation method and vesicle technology, applied in the biological field, can solve the problems of expensive, expensive ultra-high-speed centrifuges, and low purity of extracellular vesicles
CN110283776APending Publication Date: 2019-09-27SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
Publication Date
2019-09-27

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Abstract

The invention relates to the technical field of biology, in particular to a separation method of EVs (extracellular vesicles). The separation method of the EVs comprises steps as follows: 1) EV samples are subjected to centrifugal treatment to provide a liquid supernatant; 2) the liquid supernatant provided in the step 1) is subjected to electrophoresis dialysis treatment to provide an EV liquid; 3) the EV liquid provided in the step 2) is subjected to ultrafiltration treatment. The EVs are prepared and obtained with the provided separation method, the complete structure of the EVs can be kept, the prepared EVs have the advantages of high purity, low impure protein pollution and the like while multiple samples can be treated simultaneously, almost all EVs in to-be-treated samples can be obtained, and the method has good yield. Besides, the provided method comprises simple steps and is easy to operate, no expensive equipment is involved in the whole process, used consumable materials can be repeatedly recycled, and the method is a low-cost and efficient treatment method.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for isolating extracellular vesicles. Background technique

[0002] Extracellular vesicles (exosomes) are membranous vesicles that can be produced and secreted by almost all living cells, and have important applications in the diagnosis and treatment of diseases.

[0003] The isolation of extracellular vesicles is critical for their downstream applications. At present, the separation methods of extracellular vesicles mainly include the following: 1. Ultracentrifugation speed (Théry C et al.Curr Protoc Cell Biol, 2006; 3:1-29) or density gradient centrifugation (Tauro BJ et al. Methods 2012; 56:293–304.), this method is considered to be the gold standard for extracellular vesicle isolation, but this method requires an expensive ultra-high-speed centrifuge, so it is not easy to popularize. 2. Coagulation and sedimentation method, this method can be divided into two categorie...

Claims

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