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A kind of anti-oral pathogenic bacteria polypeptide and its application

A technology for pathogenic bacteria and oral cavity, applied in the anti-oral pathogenic bacteria polypeptide and application field, can solve the problems of bacterial resistance, flora imbalance, etc., and achieve the effect of preventing and treating periodontal disease with low toxicity

Active Publication Date: 2020-10-16
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, antibiotics are mainly used in the treatment of periodontal diseases to inhibit and kill pathogenic bacteria such as Porphyromonas gingivalis and Nucleobacterium spp. Normal flora in the oral cavity and gastrointestinal tract, leading to dysbiosis and related diseases

Method used

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  • A kind of anti-oral pathogenic bacteria polypeptide and its application
  • A kind of anti-oral pathogenic bacteria polypeptide and its application
  • A kind of anti-oral pathogenic bacteria polypeptide and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Screening and obtaining of peptides against oral pathogenic bacteria

[0016] The antibacterial polypeptide proposed in the present invention is derived from the Chinese medicine grub polypeptide sequence database constructed by us, and its amino acid sequence is as follows: -Gln-His, as shown in SEQ ID NO:1.

[0017] According to the amino acid sequence shown, the corresponding antibacterial polypeptide was synthesized by Gill Biochemical (Shanghai) Co., Ltd., and its purity> 95%.

Embodiment 2

[0019] Antibacterial activity of peptides against oral pathogens

[0020] The polypeptide is configured to a certain concentration and stored for later use, and the physiological salt is used as the diluent, and a series of gradient polypeptide solutions are configured by the double dilution method. Adjust the bacterial solution to be tested in the logarithmic growth phase to about 10 with fresh medium 5 -10 6 CFU / mL is used for inoculation. The medium used for Porphyromonas gingivalis and nucleobacteria is ATCC Medium 2722, and the medium used for normal strains is LB. Take 100 μL of the above-mentioned peptide solution and bacterial solution and add them to a sterile 96-well cell culture plate, 3 parallel for each peptide concentration. Constant temperature culture at 37°C, anaerobic culture of Porphyromonas gingivalis and nucleobacillus bacteria for 48h, normal culture of conventional bacteria for 18-24h, the lowest peptide concentration with no visible bacterial growth to the...

Embodiment 3

[0026] Effectiveness of antibacterial effect of peptides against oral pathogens

[0027] Adjust the bacterial solution to be tested in the logarithmic growth phase to about 10 with fresh medium ATCC Medium 2722 5 -10 6 CFU / mL, add antibacterial peptide, the final concentration of antibacterial peptide is 100μg / mL, without adding antibacterial peptide as control. Incubate at 37°C at a constant temperature, and cultivate Porphyromonas gingivalis and nucleobacillus anaerobic for 120 hours. Observe bacterial growth and detect the OD630 absorbance of the culture system.

[0028] The antibacterial effectiveness of antibacterial peptide figure 1 As shown in the figure, A: Porphyromonas gingivalis without polypeptide treatment; B: Porphyromonas gingivalis plus polypeptide treatment; C: Nucleobacteria plus polypeptide treatment; D: Nucleobacteria without polypeptide treatment.

[0029] by figure 1 It can be seen that the culture system of Porphyromonas gingivalis (test tube B) and nucleobacil...

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Abstract

The invention belongs to the field of medical application and particularly relates to a polypeptide resistant to oral pathogens and application. The amno acid sequence of the polypeptide is Gly-Phe-Gly-Arg-Ile-Gly-Arg-Ile-Val-Leu-Arg-Asn-Ala-Leu-Gln-His and is shown as SEQ ID NO:1. The polypeptide resistant to porphyromonas gingivalis and fusobacteriumnu cleatum is suitable for being prepared intodrugs, mouthwash and toothpaste additives for treatment of porphyromonas gingivalis and fusobacteriumnu cleatum infectious diseases, and has a strong killing effect on porphyromonas gingivalis and fusobacteriumnu cleatum and minimal toxicity to red blood cells of human blood.

Description

Technical field [0001] The invention belongs to the field of medical applications, and specifically relates to an anti-oral pathogenic polypeptide and its application. Background technique [0002] The oral pathogenic bacteria Porphyromonas gingivalis and Nucleobacillus play an important role in the occurrence and development of periodontitis, invasive periodontitis, periodontal abscess, and dental pulp infection, and also cause bad breath The main pathogenic bacteria. At present, antibiotics are mainly used in the treatment of periodontal diseases to inhibit and kill pathogenic bacteria such as Porphyromonas gingivalis and nucleobacillus. The long-term use of these antibiotics will not only induce the bacteria to develop resistance, but also kill some of them. The normal flora in the oral cavity and gastrointestinal tract leads to imbalance of the flora and causes related diseases. Antimicrobial peptides, as the star molecule in the research and development of new antibacteria...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08A61K38/10A61P1/02A61P31/04A61K8/64A61Q11/00A61Q17/00
CPCA61K8/64A61K38/00A61P1/02A61P31/04A61Q11/00A61Q17/005C07K7/08
Inventor 李钟杰高社干邓博
Owner HENAN UNIV OF SCI & TECH
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