53 results about "Gingivalis porphyromonas" patented technology

The invention is directed to vaccine compositions and methods based on P. gingivalis proteins identified to be regulated by haem availability that can be used in the prevention and treatment of periodontal disease. In particular, two specific internalin-like P. gingivalis proteins, namely PG0350 and PG1374 involved in the internalization of P. gingivalis by host cells, the hypothetical protein, PG1019 purported to be a cell surface lipoprotein and the alkyl hydroperoxide reductase protein, PG0618 have been identified as useful targets for the prevention and treatment of periodontal disease.

The invention relates to generation and use of cellular and humoral responses for the prevention and treatment of P. gingivalis related conditions and diseases.

The invention discloses an anti-porphyromonas gingivalis and fusobacterium nucleatum compound specific IgY preparation. According to the preparation, 10-70 parts by weight of porphyromonas gingivalis thalli and thalli fragments, and 30-90 parts by weight of fusobacterium nucleatum thalli and thalli fragments are compounded into an antigen to immune an egg laying hen. The invention also discloses the specific preparation method of the anti-porphyromonas gingivalis and fusobacterium nucleatum compound specific IgY preparation, and toothpaste containing the anti-porphyromonas gingivalis and fusobacterium nucleatum compound specific IgY preparation. By the IgY toothpaste which is used for treating and preventing gingivitis and ozostomia has obvious effects in preventing and treating the gingivitis and the ozostomia.

The invention relates to the technical field of microorganisms, and discloses lactobacillus plantarum for preventing and/or treating periodontitis as well as a culture, preparation and application of the lactobacillus plantarum. The name of the lactobacillus plantarum is Lactobacillus plantarum CCFM1137, the lactobacillus plantarum is preserved in Guangdong Microbial Culture Collection Center on the 5th floor of the Building 59, No. 100 Courtyard, Xianlie Middle Road, Guangzhou on August 1, 2020, and the preservation number is GDMCC No: 61114. The lactobacillus plantarum can be used for inhibiting the formation of a mixed bacterium biological membrane of porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum; the content of inflammatory factors in a periodontitis cell model can be reduced; the expression quantity of transmembrane transport protein in a periodontitis cell model can be increased; and the lactobacillus plantarumcan be used for preventing and/or treating oral diseases and inhibiting prevotella intermedia, porphyromonas gingivalis and/or fusobacterium nucleatum.

The invention discloses an antibacterial, bacteriostatic and halitosis-resistant toothpaste prepared by mixing natural extract and vitamin C. The antibacterial, bacteriostatic and halitosis-resistanttoothpaste comprises the natural extract and a vitamin C mixture, wherein the natural extract comprise liquorice, grape seed extract, scutellaria baicalensis, ginkgo leaf extract and grapefruit according to a mass ratio of the liquorice to the grape seed extract to the scutellaria baicalensis to the ginkgo leaf extract to the grapefruit is 1:3:2:5:2; and the vitamin C mixture comprises anhydrous citric acid, vitamin C, ascorbic acid vitamin C, refined salt and lactic acid. The antibacterial, bacteriostatic and halitosis-resistant toothpaste prepared by mixing the natural extract and the vitamin C has a very outstanding preservative effect on escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, candida albicans and aspergillus niger strains, has an antibacterial effect on porphyromonas gingivalis, streptococcus mutans and helicobacter pylori strains, can be used as an antibacterial agent in an oral cavity, can replace an existing chemical preservative, and is simple in process of producing a large amount of natural preservatives.

The invention discloses agilawood toothpaste which comprises the following components in parts by weight: 10-60 parts of a friction agent, 30-50 parts of a humectant, 0.5-2 parts of a thickening agent, 1-3 parts of a foaming agent, 0.1-5 parts of a functional agent and 0.2-3 parts of water, and the functional agent comprises an agilawood extract and an anti-allergy agent. The agilawood extract is one or a mixture of two of an agilawood leaf extract and an agilawood flower extract in any proportion; and the raw materials of the anti-allergy agent comprise baking soda, a glaze protein extract and calcium phosphate. The invention further discloses a preparation method of the agilawood toothpaste. The agilawood toothpaste disclosed by the invention can inhibit porphyromonas gingivalis and klebsiella pneumoniae in the oral cavity of a smoker, and can prevent hemodia and dental caries.

The invention discloses a medical oral cavity antibacterial composition, a preparation method and application thereof. The composition is prepared from the following components in parts by weight: (2to 20)*10<-4 > parts of polyhexamethylene biguanide, 15 to 30 parts of cypress essential oil and 0.01 to 20 parts of a Chinese herbal medicine extract, wherein the Chinese herbal medicine extract is selected from one, two or more of a ginkgo leaf extract, a honeysuckle extract, a salvia officinalis extract and a gardenia fruit extract. The product provided by the invention has good stability and excellent antibacterial and bacteriostatic ability, especially can effectively inhibit porphyromonas gingivalis in the oral cavity, can inhibit and relieve periodontitis, and can be prepared into mouthwash and gel, wherein the preparation can reach the medical grade.

The present disclosure discloses a medium for primary isolation of Porphyromonas gingivalis, and a method for preparing the medium and use thereof. The medium for primary isolation of Porphyromonas gingivalis includes the following components in parts by weight: 10-20 parts of a mixed peptone, 5-10 parts of a yeast extract powder, 1-5 parts of sodium chloride, 10-15 parts of agar, 0.5-1.0 parts of glucose, 0.2-0.8 parts of sodium bicarbonate, 0.1-0.5 parts of an L-cysteine salt, 0.1-0.5 parts of soluble sodium pyrophosphate, 0.0001-0.0005 parts of hemin, 0.00001-0.00005 parts of vitamin K, 500-1000 parts of water and 5-10 parts of a sterile defiberized sheep blood. By adopting the medium for primary isolation of Porphyromonas gingivalis provided by examples of the present disclosure, a culture period of primary isolation of Porphyromonas gingivalis can be greatly shortened, and the primary isolation of Porphyromonas gingivalis can be quickly conducted.

The invention discloses lactobacillus fermentum capable of preventing and/or treating periodontitis and application of the lactobacillus fermentum, and belongs to the technical field of microorganisms. The lactobacillus fermentum CCFM1139 is obtained by screening, and the lactobacillus fermentum CCFM1139 has the effect of relieving periodontitis, which is specifically embodied in that the lactobacillus fermentum CCFM1139 can inhibit the formation of a porphyromonas gingivalis, Prwinia intermediary and fusobacterium nucleatum mixed biological membrane, so the quantity of the biological membrane is reduced by 52.45%; The content of TNF-alpha in a periodontitis cell model can be reduced from 131.37 pg/mL to 83.31 pg/mL, and the content of IL-8 in the periodontitis cell model can be reduced from 147.70 pg/mL to 121.12 pg/mL; The colonization quantity of the porphyromonas gingivalis and the fusobacterium nucleatum in the oral cavity is reduced by 1-2 orders of magnitude; and the alveolar bone absorption amount is reduced from 1838.0 [mu] m to 805.7 [mu] m.

The invention provides a composition for regulating oral flora balance and a preparation method and application thereof, the composition comprises conventional components and probiotic components, and the probiotic components comprise fructo-oligosaccharide and lactobacillus paracasei. The composition for protecting the oral cavity has the beneficial effects that the composition for protecting the oral cavity is provided, the composition contains the probiotic component formed by combining the fructo-oligosaccharide and the lactobacillus paracasei, and the two components have a synergistic effect together; according to the oral care solution, beneficial bacteria can be obviously proliferated, the inhibition rate on malignant bacteria such as anaerobic bacteria, streptococcus mutans, fusobacterium nucleatum, actinomycetes-associated haemophilus, porphyromonas gingivalis, streptococcus gingivalis groups, Wehonor flora and neisseria flora is improved, and various oral problems are solved.

The invention discloses toothpaste for oral ulcer. The toothpaste comprises the following components in percentage by weight: 20-40% of an abrasive, 30-60% of sorbitol, 2.0-3.0% of a surfactant, 0.1-0.3% of menthol, 0.01-1.0% of sodium gualenate, 0.2-1.0% of dipotassium glycyrrhizinate, 0.8-1.3% of a thickening agent, 0.1-0.4% of sucralose, 0.8-2.0% of food-grade essence, 0.5-1.0% of a honeysuckle flower extract and the balance of purified water. The toothpaste for oral ulcer takes sodium gualenate as a key functional component; sodium gualenate is safe and non-toxic; the killing rate of sodium gualenate on streptococcus mutans, staphylococcus aureus, candida albicans and escherichia coli reaches 99.98% or above; the killing rate of sodium gualenate on porphyromonas gingivalis reaches 98.14% or above; and the toothpaste has the remarkable effects of inhibiting dental plaque, relieving gingivitis and promoting healing of recurrent oral ulcer.

The invention discloses amino acid toothpaste containing NMN and a preparation method of the amino acid toothpaste, and belongs to the field of oral care products. The amino acid toothpaste is prepared from the following components in percentage by mass: 100% of deionized water, 3-17% of a foaming agent, 10-40% of a friction agent, 10-45% of a humectant, 0.1-10% of a thickening agent, 0.1-1% of a sweetening agent, a proper amount of a preservative, 0.5-2% of NMN, 0.5-6% of a lactobacillus fermentation product and 0.01-0.3% of a mangnolia officinalis bark extract. The amino acid surfactant is used as a foaming agent, and compared with traditional toothpaste using sodium lauryl sulfate (K12), the toothpaste is safer and milder. The toothpaste disclosed by the invention has a bacteriostatic effect on common oral pathogenic bacteria such as porphyromonas gingivalis and the like, is safer compared with a traditional bactericide, does not influence oral micro-ecological groups, effectively competitively inhibits proliferation of pathogenic bacteria, promotes proliferation of beneficial bacteria and improves oral health, and is suitable for various people.

The invention discloses a compound oral care solution. The compound oral care solution is prepared from the following raw materials in parts by weight: 0.15 to 0.3 part of blumea balsamifera oil, 0.2 to 0.3 part of callicarpa nudiflora extract, 0.05 to 0.1 part of alpinia oxyphylla oil, 10 to 15 parts of glycerol, 3 to 6 parts of ethanol, 2 to 5 parts of sorbitol, 1 to 2 parts of tween-20, 0.01 to 0.02 part of citric acid, 0.2 to 0.35 part of saccharin sodium salt, 0.25 to 0.35 part of peppermint oil, 0.1 to 0.3 part of essence and 20 to 50 parts of water. The invention further discloses a preparation method of the compound oral care solution. According to the compound oral care solution and the preparation method thereof, the compound oral care solution is highly sensitive to staphylococcus aureus, candida albicans and streptococcus mutans, is sensitive to escherichia coli and porphyromonas gingivalis, and can well prevent colonization of oral pathogenic bacteria on a dental acquired membrane, thereby avoiding formation of dental plaque and dental calculus.

The invention relates to a specific anti-porphyromonas gingivalis egg yolk antibody liposome solution and a preparation method thereof, and belongs to the field of medicines for treating periodontitis. Each milliliter of the egg yolk antibody liposome solution contains 4mg of egg yolk antibody liposome, and the egg yolk antibody liposome is a multi-chamber liposome; the egg yolk antibody liposomesolution is a fluid at a temperature lower than room temperature and forms gel at a temperature of 37 DEG C, so that the requirements of injection administration and in-vivo gelatinization in the periodontal pocket are met, the concentration in the periodontal pocket is effectively improved, and the dose is reduced. The preparation method comprises the following steps: using lecithin and cholesterol as raw materials and tocopherol as a stabilizer, and preparing a liposome film by a film dispersion method; coating the egg yolk antibody with the liposome film to prepare an egg yolk antibody liposome capsule, and reforming the formed liposome capsule by using a homogenizer to obtain the specific anti-porphyromonas gingivalis egg yolk antibody liposome solution, wherein the egg yolk antibody liposome is a nano-scale liposome.

The invention discloses a composition for resisting oral pathogenic bacteria and a preparation method and application thereof. According to the invention, the propolis extract and the traditional Chinese medicine water-decoction extract are uniformly mixed according to a mass ratio of (1-4):(1-4) to obtain the composition for resisting oral pathogenic bacteria. The test research of the invention shows that: the compounding of the propolis extract and the traditional Chinese medicine water-decoction extract has high-efficiency inhibition effect on the growth of oral pathogenic bacteria streptococcus mutans and porphyromonas gingivalis, the compounding of the two extracts has synergistic effect, and the compounding of the propolis extract and the traditional Chinese medicine water-decoctionextract in a mass ratio of 1:1has better bacteriostatic effect. The composition for resisting oral cavity pathogenic bacteria has the advantages of natural and environment-friendly raw materials, remarkable effect, benefit to human health, and capability of being applied to prevention and treatment of periodontitis and dental caries.

The invention discloses a soothing and nourishing toothpaste. The toothpaste comprises the following components in parts by weight: 0-90 parts of a solvent, 1-90 parts of a humectant, 0-90 parts of anabrasive, 0.1-30 parts of a thickener, 0-20 parts of a foaming agent, 0-10 parts of a sweetening agent, 1-20 parts of a plant compound extract and 0.1-50 parts of a functional agent. The invention further discloses a preparation method of the soothing and nourishing toothpaste. The toothpaste has the beneficial effects that the functional agent and the original abrasive of the toothpaste take effect synergistically, so that the toothpaste has very good repairing and brightening effects while having good cleaning and whitening effects; the added plant compound extract has excellent and long-lasting inhibition on Porphyromonas gingivalis, Streptococcus mutans and Fusobacterium nucleatum, also has an anti-inflammatory effect, can effectively assist in treating and preventing oral inflammation, keeps fresh breath, eliminates halitosis and has a good soothing feeling; the toothpaste has a good repairing effect on injury of human gingival cells; and the toothpaste has very good maintenanceeffects of moisturizing the oral cavity and the like.

Provided are a cra4S1 gene, an encoded cra4S1 protein, and a vaccine or drug containing the cra4S1 protein or a fragment thereof. A nucleotide sequence of the cra4S1 gene is represented by SEQ ID NO. 1. The vaccine combines the specific target of an outer membrane protein of Porphyromonas gingivalis and the antigen component of the bacterial conserved region, which has an immune prevention and protection effect on the body.

The invention discloses a processing technology of mouthwash for improving oral health. The mouthwash contains a tea leaf concentrated solution, an antrodia camphorata concentrated solution, a perfumeand a menthol solution, wherein the antrodia camphorata concentrated solution forms antrodia cinnamomea mycelium extract, the mass percentage of the tea leaf concentrated solution is 20-80%, the masspercentage of the antrodia camphorata concentrated solution is 10-50% the mass ratio of the perfume is 1-5%, and the mass ratio of the menthol solution is 0.1-5%. The mouthwash can effectively inhibit the growth of porphyromonas gingivalis and streptococcus muntans bacteria or kill the porphyromonas gingivalis and the streptococcus muntans bacteria; the antrodia camphorata concentrated solution is extracted from antrodia cinnamomea mycelia, so that the antrodia camphorata concentrated solution is easy to obtain; and the mouthwash does not have the side effect problem of chemical synthetics, so that the mouthwash can be easily applied to various industries, especially such as health foods, beverages, living goods and the like.

The invention discloses a composite periodontitis accompanied tooth movement mouse modeling method which comprises the following steps: comprehensively establishing a mouse periodontitis model through rough surgical suture and porphyromonas gingivalis bacterial liquid, and establishing a periodontitis accompanied tooth movement model after confirming that the periodontitis model is successfully established through local periodontal tissue pathological change. On the basis of a mild and injury-free periodontitis model, a unique mouse posterior tooth movement method is combined, pathological changes of periodontal tissues in the correction process under the condition of clinical adult orthodontic treatment with periodontal problems are successfully simulated, and the periodontal position change condition is determined. The invention innovatively provides the mouse model for compounding periodontitis with tooth movement, effectively fills the blank of animal models in the related research field of periodontitis and orthodontic tooth movement, shows great application value in discussing the complicated pathological mechanism associated with periodontitis and orthodontic tooth movement, and broadens the idea and method of the clinical research hotspot.

The invention relates to the field of biological medicines, in particular to monoclonal antibodies, a test strip and a kit for specifically recognizing porphyromonas gingivalis. According to the invention, by using P.gingivalis and rHA2 immune mice, monoclonal antibodies for recognizing different sites of P.gingivalis are prepared, and paired antibodies are screened from the monoclonal antibodiesto construct a double-antibody sandwich ELISA system for specifically recognizing porphyromonas gingivalis. The paired antibodies obtained by screening are used for preparing the simple colloidal metal test strip, and the simple colloidal metal test strip can be used for quickly identifying 109CFU/mL P.gingivalis. The invention has the advantages that: the kit is simple and rapid, the result can be obtained within 2 minutes, and the result can be observed by naked eyes without any instrument and equipment.