A kind of composite microbial bacterial agent and its application
A technology of composite microbial agent and bacillus, which is applied in the direction of microorganisms, biocides, and methods based on microorganisms, can solve the problems of single effect, poor stability, and unsatisfactory effect of repairing heavy metals, achieving stable effects and wide application range , the effect of preventing and controlling plant wilt
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[0033] In the present invention, the preparation method of the solid bacterial powder preferably includes the following steps: activating the bacterial species and performing continuous two-stage fermentation and concentration to obtain a concentrated bacterial liquid; mixing the concentrated bacterial liquid with a carrier and spray drying The solid bacterial powder is obtained.
[0034] In the present invention, the strain is inoculated in the NB liquid medium and cultured for 12-16 hours to obtain the first-level seed liquid; the temperature of the culture is preferably 37°C, the rotation speed of the culture is preferably 200 rpm; the time of the culture is preferably For 14h.
[0035] In the present invention, after obtaining the first-level seed liquid, the first-level seed liquid is transferred to the NB liquid medium and cultured for 8-12 hours to obtain the second-level seed liquid. In the present invention, the inoculum amount for the first-level seed solution transfer i...
Embodiment 1
[0044] Preparation of Bacillus pumilus SEM-7 bacterial liquid and solid bacterial powder:
[0045] Activate the Bacillus pumilus SEM-7 strain, pick a single colony, and inoculate it in 100 mL of NB liquid medium (at 121°C, sterilization for 20 min), and incubate at 37°C and 200 rpm for 14 hours to obtain a first-level seed solution.
[0046] Connect the first-level seed liquid into a sterile seed tank, the inoculum amount is 0.7-1% (v / v); the culture conditions are: aeration ratio 1:1, stirring speed 200-400rpm, culture temperature 37℃, tank The pressure is 0.05MPa, the culture time is 10h, and the secondary seed liquid is obtained at the end of the culture. The content of viable bacteria in the secondary seed liquid is 10 7 cfu / mL, the OD value is 0.8~0.9, at this time the strain is strong.
[0047] Fermentation of Bacillus pumilus SEM-7
[0048] Connect the secondary seed liquid of Bacillus pumilus SEM-7 into a sterile fermentor at an inoculum of 0.5-1% (v / v). The composition of the...
Embodiment 2
[0060] Adsorption experiment of compound microbial agents on heavy metals cadmium and chromium
[0061] Inoculate NB medium with compound microbial agent 1:1000 (mass percentage), shake it overnight at 37°C, prepare bacterial solution and heavy metal cadmium and chromium standard solution to strain concentration 10 8 Cfu / mL, cadmium, chromium final concentration of 50mg / L and 100mg / L mixed culture medium respectively, mix, let stand for 5-6h, centrifuge at 4000rpm for 5min, take the supernatant, control and mixed culture medium respectively set three replicates , Using atomic absorption spectrometry to detect the content of soluble cadmium and chromium in the supernatant. The results showed that the cadmium and chromium concentrations in the supernatant were 20.7±1.59 mg / L and 49.7±4.32 mg / L after the compound bacterial agent treatment, which were 51.1±0.54 mg / L and 89.4±2.55 mg in the blank supernatant. / L, the adsorption efficiency of the composite microbial agent of the presen...
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