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Device and method for separating mobile cells

A mobile cell and cell technology, applied in separation methods, animal cells, vertebrate cells, etc., can solve problems such as sample contamination, inability to use in the field or consulting room, material loss, etc.

Pending Publication Date: 2019-10-11
SELECTIVITY SAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The devices and methods present in the prior art have technical disadvantages compared to the present invention, since the culture medium used for the separation can become clogged, they need to be handled by persons relatively skilled in the handling of laboratory instruments, and thus also require Processed in a controlled environment so cannot be used on-site or in consulting rooms
On the other hand, most of them require additional filtration operations and / or centrifugation operations, whether due to increased sample handling time or stress caused by rotation during centrifugation, leading to sample contamination, material loss, DNA alteration, etc. possibility

Method used

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  • Device and method for separating mobile cells

Examples

Experimental program
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Effect test

example 1

[0053] Device test D, E and F with glass film

[0054] On a glass membrane, the mobile cell separation device (MXM) of the present invention includes two reservoir plates separated by a 130±40 μm thick glass membrane plate, which has tapered channels with a diameter of about 1 mm. The length of the glass width, a first diameter of 15 μm (inlet) and a second diameter of 8 μm (outlet). First place the HEPES recovery medium HamF10 1X, supplement with SSS, and place penicillin / streptomycin in the recovery reservoir (second reservoir); then, after processing according to the WHO2010 protocol, semen samples D, E and F It is placed in the remaining storage (first storage).

[0055] In this embodiment, the membrane is 1cm×2cm and the density is 6600 perforations / cm 2 . The maximum volume capacity of the first reservoir for placing the sample to be processed is 3 mL, and the maximum capacity of the second reservoir is 1 mL.

example 2

[0057] Tests A, B, C, and D for devices with PET film

[0058] A film made of PET is formed with 2500 channels, and the length of each channel is 200 μm. The conical channel includes a first (inlet) diameter of 15 μm and a second (outlet) diameter of 10 μm. The PET film is 1cm×2cm. The storage is the same as the storage corresponding to the glass film in Example 1. First place the HEPES recovery medium Ham F10 1X, supplement with SSS, and place penicillin / streptomycin in the recovery reservoir (second reservoir); then, after processing according to the WHO2010 protocol, semen samples A, B, C and D are placed in the remaining storage (first storage).

[0059] For these two examples, the procedure for using the device of the present invention is as follows:

[0060] Step 1-Place the HEPES medium Ham F10 1X (0.6-0.7 ml) to reach the level of the second reservoir. Load Ham F10 1X with HEPES with a straw-tip Buster transfer tube that can enter the analysis cup; when it is filled, the...

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Abstract

The invention relates to a device for separating mobile cells, formed by two reservoirs that share a side formed by a perforated plate in which at least one channel has a narrowed diameter, adopting aconical shape. A sample containing the cells is deposited in the first reservoir and the second reservoir is filled with a culture medium, with a level mark higher than that of the first reservoir, producing a current towards same. The device is used to select, in a semen sample, the most suitable spermatozoa for use in assisted reproduction techniques.

Description

Technical field [0001] The present invention describes a device and method for separating active cells from cell populations. In particular, it is used in the sperm recovery technology of assisted reproductive technology (ART), which requires sperm motility, morphology and DNA integrity (1) to recover a certain quality and quantity of sperm without other devices . Background technique [0002] There are many methods and devices for cell separation in the prior art. These methods and devices are based on the characteristic properties (such as fluidity, etc.) of mobile cells as sperm (4). Devices and methods for separation in the prior art use filter membranes, density gradients, perforated membranes that block the passage of cells, and the like. [0003] Patents US6129214(A) and US6357596B1 describe methods and devices for separating optimal sperm using membranes, in which sperm that penetrate the membrane can be maintained in a suitable medium. According to the instructions, th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/076A61B17/425A61D19/00
CPCA61D19/00C12M1/12C12N5/00G01N1/34C12N5/0612B01D29/085C12M47/04
Inventor H·E·H·雷佩托
Owner SELECTIVITY SAS
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