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Light-drug combination induced artificial breeding method for capitulum mitella linnaeus juveniles

A combination method and turtle foot technology, applied in the field of artificial seedling induction of turtle foot juveniles by photopharmaceutical combination method, can solve the problems that the mechanism of action of juvenile hormone has not been fully revealed, and achieve remarkable seedling effect, stable effect, and simple operation Effect

Active Publication Date: 2019-10-15
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a lot of research has been done on the molecular mechanism of juvenile hormone in recent years, the mechanism of action of juvenile hormone is far from being fully revealed

Method used

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  • Light-drug combination induced artificial breeding method for capitulum mitella linnaeus juveniles
  • Light-drug combination induced artificial breeding method for capitulum mitella linnaeus juveniles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. The preparation of the mutagen for the attachment and metamorphosis of the tortoise cycad:

[0034] According to the weight volume ratio of S-methoprene: dimethyl sulfoxide of 1:40~50 (w / v), mix and dissolve evenly, add ethanol to prepare a mutagen containing S-methprene 1mg / mL , stored at 4°C;

[0035] 2. Mutagenesis breeding pool:

[0036] The structure of the mutagenesis cultivation pool described in this embodiment is shown in the technical scheme. The mutagenesis breeding pool is composed of three pools of the same volume, A-level pool, B-level pool, and C-level pool. Among them: the size of A-level pool, B-level pool, and C-level pool is: 1.5 meters (length) × 1.5 meters (width) × 2.0 meters (height), and the water injection height is 1.5 meters.

[0037] 3. In the A-level pool, B-level pool, and C-level pool in the mutagenesis cultivation pool, put the glandular larvae attachment device respectively, and after filling clean seawater, close the upper connect...

Embodiment 2

[0046] 1. The preparation of the mutagen for the attachment and metamorphosis of the tortoise cycad:

[0047] According to the weight volume ratio of S-methoprene: dimethyl sulfoxide of 1:40~50 (w / v), mix and dissolve evenly, add ethanol to prepare a mutagen containing S-methprene 1mg / mL , stored at 4°C;

[0048] 2. Mutation breeding pool:

[0049] The structure of the mutagenesis cultivation pool described in this embodiment is shown in the technical scheme. The mutagenesis breeding pool is composed of three pools of the same volume, A-level pool, B-level pool, and C-level pool. Among them: the size of A-level pool, B-level pool, and C-level pool is: 1.0 meters (length) × 1.0 meters (width) × 1.6 meters (height), and the water injection height is 1.3 meters.

[0050] 3. After being cultivated, the broodstock of the tortoise is laid eggs and hatched, and the hatched nauplii are collected and cultivated in a 500L black glass fiber reinforced plastic tank for 11 days. About ...

Embodiment 3

[0059] 1. The preparation of the mutagen for the attachment and metamorphosis of the tortoise cycad:

[0060] According to the weight volume ratio of S-methoprene: dimethyl sulfoxide of 1:40~50 (w / v), mix and dissolve evenly, add ethanol to prepare a mutagen containing S-methprene 1mg / mL , stored at 4°C;

[0061] 2. Mutation breeding pool:

[0062] The structure of the mutagenesis cultivation pool described in this embodiment is shown in the technical scheme. The mutagenesis breeding pool is composed of three pools of the same volume, A-level pool, B-level pool, and C-level pool. Among them: the size of A-level pool, B-level pool, and C-level pool is: 1.0 meters (length) × 1.0 meters (width) × 1.3 meters (height), and the water injection height is 1.0 meters.

[0063] 3. After being cultivated, the broodstock of the tortoise is laid eggs and hatched, and the hatched nauplii are collected and cultivated in a 500L black glass fiber reinforced plastic tank for 10 days. About ...

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Abstract

The invention relates to a light-drug combination induced artificial breeding method for capitulum mitella linnaeus juveniles. The method comprises steps as follows: S-hydroprene and dimethyl sulfoxide are mixed, ethanol is added, and an inducer containing S-hydroprene with concentration of 1 mg / mL is prepared; a drip pipe is opened to add the inducer dropwise to a grade-A pool; an LED lamp of a grade-B pool is turned on and is turned off after 2 h, and a drip pipe of the grade-B pool is opened to add the inducer dropwise; an LED lamp of a grade-C pool is turned on and is turned off after 2 h,and a drip pipe of the grade-C pool is opened to add the inducer dropwise continuously to the grade-C pool; upper overflow pipes among the pools are closed, lower drain pipes among the pools and a drain pipe of the grade-C pool are opened, fresh seawater is injected into the grade-A pool by a water injection pipe, meanwhile, the drip pipes are opened, the adding is stopped after 4-6 days, and a juvenile breeding stage starts. The method is simple and feasible and has a good effect, the incubating rate of the capitulum mitella linnaeus juveniles can reach 59.13% or higher, the highest incubating rate is 76%, the harvested juveniles can grow normally to adulthood, and artificial breeding of capitulum mitella linnaeus becomes possible.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to an artificial seedling method for inducing tortoise juveniles by combining light and medicine. Background technique [0002] Turtle foot (Capitulum mitella Linnaeus) is a mariculture species with high economic value and development prospects, belonging to Arthropoda, Crustacea, Cirripedes, Peritus, Stems, and Armorophyta , Refers to the genus Capitulum of the family Pollicipidae. Turtle feet are commonly known as pen holders, bergamot, dog claw snails, etc., and generally live in rock crevices in the middle and high tide areas where the waves are violently impacting and the water flow is smooth. Adhesive to rock or other surfaces. Due to the constraints of the habitat environment, the population of tortoise feet is small and the size of individuals is mixed, and the larvae are often attached to the stalk of the adult; when harvesting, clusters of individuals are dug out, an...

Claims

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Application Information

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IPC IPC(8): A01K61/59A01K63/00A01K63/04A01K63/06
CPCA01K61/59A01K63/003A01K63/06A01K63/042A01K63/04Y02A40/81
Inventor 林岗饶小珍
Owner FUJIAN NORMAL UNIV
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