Photocrosslinking double-modified silk fibroin-gelatin composite bio-ink as well as preparation method and application thereof

A silk fibroin, bio-ink technology, applied in the directions of additive manufacturing, manufacturing tools, metal processing equipment, etc., can solve the problems of brittle large gel structure and other problems, achieve a high degree of automation, good compression resistance and elasticity, enhanced The effect of mechanical properties

Inactive Publication Date: 2019-10-15
DONGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Its hydrogel morphology has been widely used and developed because of its many excellent physical and chemical properties, diversified gelation methods, and the rich water environment inside can simulate the three-dimensional microenvironment of cells, but it is also because of its high brittleness. , special gel structure and other factors limit its application in various fields

Method used

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  • Photocrosslinking double-modified silk fibroin-gelatin composite bio-ink as well as preparation method and application thereof
  • Photocrosslinking double-modified silk fibroin-gelatin composite bio-ink as well as preparation method and application thereof
  • Photocrosslinking double-modified silk fibroin-gelatin composite bio-ink as well as preparation method and application thereof

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Embodiment 1

[0036] (1) After dissolving the boiled silk fibroin fibers with LiBr, 424mM GMA solution was added to the mixture solution, and reacted at 60° C. for 3 hours at a speed of 300 rpm to produce a high-yield reaction. After filtering, use a dialysis bag with a molecular weight of 12-14kDa to dialyze for 4 days, replace the deionized water every 4h, then place it in a -80°C refrigerator overnight, and obtain methacrylylated silk fibroin (SilMA) after freeze-drying. Store at -4°C for use;

[0037] (2) Dissolve 10 g of gelatin in 100 mL of deionized water, let stand at room temperature for 1 h, and then stir in a constant temperature water bath at 60°C until completely dissolved. Under light-shielded conditions, 5 mL of methacrylic anhydride was added dropwise to the gelatin solution at a rate of 1 mL / min at 60° C., and the stirring speed was 300 rpm. After mixing evenly, shake and react in a constant temperature shaker at 50° C. for 3 h, and then add 400 mL of deionized water at 50...

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Abstract

The invention relates to a photocrosslinking double-modified silk fibroin-gelatin composite bio-ink as well as a preparation method and application thereof. The photocrosslinking double-modified silkfibroin-gelatin composite bio-ink is prepared by respectively modifying silk fibroin and gelatin by methacrylation and performing photocrosslinking. The photocrosslinking double-modified silk fibroin-gelatin composite bio-ink is good in biocompatibility; the cross-linking of the bio-ink can be enhanced by a two-component modification method, and the bio-ink has temperature sensitivity and shear thinning, and can be used as 3D printing bio-ink to construct a personalized macroporous support to meet various needs.

Description

technical field [0001] The invention belongs to the field of tissue engineering scaffolds, in particular to a photo-crosslinked double-modified silk fibroin-gelatin composite biological ink and its preparation method and application. Background technique [0002] Tissue engineering technology has the potential to fundamentally solve tissue and organ damage and realize its functional reconstruction. It is one of the common treatment strategies for bone substitute transplantation, and individual differences in patients are one of the key factors affecting the repair effect. Therefore, the preparation has Functional tissue engineering scaffolds that can adapt and match the injury site are an important part of this strategy. 3D printing technology is an additive manufacturing technology based on precision and speed. 3D printing technology is an additive manufacturing technology based on precision and speed. 3D printing technology can well solve the problem of implanted stents an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L89/00C08J3/24A61L27/26A61L27/50A61L27/56A61L27/58B29C35/08B29C64/112B29C64/379B33Y10/00B33Y40/00B33Y70/00
CPCA61L27/26A61L27/50A61L27/56A61L27/58B29C35/0805B29C2035/0827B33Y10/00B33Y40/00B33Y70/00B29C64/112B29C64/379C08J3/24C08J2389/00C08J2489/00C08L89/00
Inventor 何创龙张景添杨晋张骞骞王伟忠刘顶华
Owner DONGHUA UNIV
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