Method for quantitative detection of single embryo secreted proteins based on microfluidic droplets
A quantitative detection method and technology of secreted protein, which is applied in the field of quantitative detection of single embryo secreted protein based on microfluidic droplets, can solve the problem of inability to accurately detect secreted protein of a single embryo, and reduce the interference of human subjective factors and achieve high detection efficiency. Sensitivity, effect of reducing reaction time
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0030] like figure 1 As shown, a method for quantitative detection of protein secreted by a single embryo based on microfluidic droplets, is characterized in that it comprises the following steps:
[0031] a. Take a capture antibody and a labeled antibody that secrete human chorionic gonadotropin (βHCG) from a single blastocyst stage embryo, connect the capture antibody to immunomagnetic beads, and label the labeled antibody with biotin;
[0032] b. Mix the immunomagnetic beads in step a with human chorionic gonadotropin (βHCG) secreted by a single blastocyst embryo and incubate at 37°C for 30 minutes, then take out the immunomagnetic beads and wash them 3 to 5 times. Mix the magnetic beads with the labeled antibody and streptavidin-labeled β-galactosidase and incubate at 37°C for 30 minutes, then take out the immunomagnetic beads and wash them 3 to 5 times, and resuspend them with 10uL of PBS buffer after washing Immunomagnetic beads;
[0033] c. Put the respun magnetic bea...
PUM
Property | Measurement | Unit |
---|---|---|
Diameter | aaaaa | aaaaa |
Excitation wavelength | aaaaa | aaaaa |
Excitation wavelength | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com