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Dairy cow mammary gland epithelial cell injury model and construction method and application thereof

A technology of mammary epithelial cells and construction methods, which is applied in the field of construction of dairy cow mammary epithelial cell injury models, and can solve the problems of unclear changes in dairy cows' pathogenic characteristics and infection characteristics of milk proteins, etc.

Inactive Publication Date: 2019-10-25
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the pathogenicity, infection characteristics and changes in milk protein caused by Streptococcus agalactiae to dairy cows are not yet clear; research lays the groundwork

Method used

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  • Dairy cow mammary gland epithelial cell injury model and construction method and application thereof
  • Dairy cow mammary gland epithelial cell injury model and construction method and application thereof
  • Dairy cow mammary gland epithelial cell injury model and construction method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1 Construction and identification of dairy cow mammary gland epithelial cell injury model

[0027] 1 Test materials and reagents

[0028] 1.1 Test material

[0029] 1.1.1 Test equipment

[0030] CO 2 Cell culture incubator (Thermo), inverted biological microscope (Motic, AE31), constant temperature incubator (Thermo), desktop high-speed refrigerated centrifuge (US Sigma 3K15 type), ultra-clean workbench, constant temperature oscillator, automatic triple pure water distillation Appliance, electric constant temperature water bath (Shanghai Bilang Instrument Co., Ltd. HH-2 type), high-pressure steam sterilizer (TOMY, SX-700).

[0031] 1.1.2 Test reagents

[0032]Brain Heart Infusion Broth (BHI) (BD, USA), DMEM / F 12 Medium (dulbecco's modified eagle medium: Nutrient F-12Ham) (Gibco, USA), Fetal bovine serum (FBS) (Gibco, USA), HBSS-Hank's balanced salt solution (Gibco, USA), 1540- 122 penicillin / streptomycin solution (Gibco, USA), 0.25% trypsin-EDTA (1X) (Gibc...

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Abstract

The invention discloses a dairy cow mammary gland epithelial cell injury model and a construction method and application thereof. According to the invention, it discovers that streptococcus agalactiaenot only can adhere to and invade dairy cow mammary gland epithelial cells, but also has an obvious toxic effect on the dairy cow mammary gland epithelial cells to cause deformation and breakage of the cells, induce the cells to undergo apoptosis, and finally cause necrosis of the cells. Therefore, the invention provides a construction method of the dairy cow mammary gland epithelial cell injurymodel. The construction method comprises the following steps: after infecting dairy cow mammary gland epithelial cells in vitro by the streptococcus agalactiae, culturing in a CO2 cell incubator to obtain the dairy cow mammary gland epithelial cell injury model. The dairy cow mammary gland epithelial cell injury model constructed by the invention lays a foundation for researching the molecular mechanism of dairy cow mastitis caused by the streptococcus agalactiae.

Description

technical field [0001] The invention relates to a method for constructing a cell damage model, in particular to a method for constructing a dairy cow mammary gland epithelial cell damage model, and belongs to the field of constructing a dairy cow mammary gland epithelial cell damage model. Background technique [0002] Cow mastitis is generally caused by a variety of pathogenic microorganisms invading the mammary gland, and a variety of pathogenic microorganisms can be isolated from cases of dairy cow mastitis. At present, the main pathogenic bacteria Staphylococcus aureus mastitis and Escherichia coli mastitis have been discovered and reported in various countries in the world; Streptococcus agalactiae, as one of the main pathogens causing mastitis in cows, has received little attention. Its pathogenic and infectious properties are currently unknown. [0003] Streptococcus agalactiae is a Gram-positive coccus, as one of the main pathogenic bacteria causing subclinical mast...

Claims

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Application Information

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IPC IPC(8): C12N5/071C12N1/20C12R1/46
CPCC12N1/20C12N5/0631
Inventor 童津津张华蒋林树杨德莲熊本海
Owner BEIJING UNIV OF AGRI
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