Transmission type fluorescence detection imaging system applied to dPCR detector

Abstract

The invention relates to a transmission type fluorescence detection imaging system applied to a dPCR detector. Beam expansion is carried out on light emitted by an illumination source by a beam expander, a reflecting mirror reflects the expanded light and then the processed light enters a first filter sheet vertically, and then after filtering, excited light is spotted on a sample chip; and fluorescent light and excitation light that are excited by the sample are collected by a microscopic imaging system, the microscopic imaging system performs primary imaging on the sample chip, a second filter sheet carries out filtering to remove the excited light, and the pure fluorescent light is received by a receiving device. According to the invention, the large imaging range of phi 35mm is completed by using a 6.6-degree small angle of view and the amplification factor is minus 0.540 times; and application to the transmission type detection system is realized. Meanwhile, the numerical apertureis 0.106 and the resolution of channel detection of the fluorescent chip can reach 10 micrometers. The system is capable of realizing clear and excellent imaging on the fluorescent light in a wavelength range of from 485nm to 656nm; and the image quality is clear and bright. The system has advantages of compact structure, good imaging effect, large aperture, and high resolution.

Description

technical field [0001] The invention relates to a microscopic imaging technology, in particular to a transmission fluorescence detection imaging system applied to a dPCR detector. Background technique [0002] Digital PCR (polymerase chain reaction) technology is a new type of highly sensitive nucleic acid absolute quantitative detection technology, which has very important application value in the field of biomedicine, especially for gene analysis and accurate diagnosis of diseases. [0003] Since dPCR technology is a terminal analysis method, if the target DNA molecule is not limitedly diluted, the detection accuracy will be greatly reduced, and the sensitivity and accuracy of dPCR will increase with the increase in the number of reaction channels. Currently, 20,000 or even more than 20,000 reaction channels have been widely used in dPCR detection and application through droplet microfluidic technology, but the increase in reaction units also increases the difficulty of de...

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the current high-throughput dPCR gene chip imaging using splicing imaging, resulting in low detection efficiency and easy introduction of splicing errors, and proposes a transmission fluorescence detection and imaging system applied to dPCR detectors, which simultaneously realizes a large field of view and high resolution

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