Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Exogenous substance importing method suitable for ova of shellfish mollusks

A mollusk and exogenous material technology, applied in the field of transgenic, to achieve the effect of clear expression and improved import rate

Active Publication Date: 2019-11-01
OCEAN UNIV OF CHINA
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the egg cells of bivalve mollusks and other marine organisms are small, mostly at the micron level, this also brings certain challenges to researchers, such as the preparation of cell carriers, the hardness of injection needles and the selection of drawing parameters, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Exogenous substance importing method suitable for ova of shellfish mollusks
  • Exogenous substance importing method suitable for ova of shellfish mollusks
  • Exogenous substance importing method suitable for ova of shellfish mollusks

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] This laboratory is equipped with dwarf clam model culture system, and this test takes the egg cell (approximately 50 μm in diameter) of the dwarf clam obtained by induced labor as an example to further describe the present invention in detail, and the steps are as follows:

[0047] (1) Suction filter the seawater with a 0.2 μm filter membrane, and sterilize it in an environment of 121° C. for 21 minutes, and reserve it; System; heat the mixed system in a microwave oven until the solution is clear to obtain a mixed solution; take 5ml of the mixed solution and pour it into a 60mm cell culture dish cover, shake well to ensure that the glue surface is smooth, and obtain a liquid gel.

[0048](2) Wipe the coverslip with anhydrous ethanol and dry it, and wrap it 5 times along the width direction of the coverslip with a 50 μm metal wire ( Figure 1a ), and lightly cover the coverslip wrapped with a metal wire in the seawater agarose liquid gel, it is advisable to immerse the bo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses an exogenous substance importing method suitable for ova of shellfish mollusks. The exogenous substance importing method comprises the steps of preparing liquid gelatin, preparing a cell carrier, pulling a needle, performing cell distribution, performing injection pretreatment, and performing microinjection. The method is used, so that exogenous genes or protein can be imported into ovum cells, and the importing positive rate of exogenous substances can be increased.

Description

technical field [0001] The invention relates to the field of transgenic technology, and more specifically relates to a method for introducing exogenous substances into eggs of shellfish molluscs. Background technique [0002] As the basic constituent unit and functional unit of organisms, cells are common research objects of micromanipulation. In the 1970s, genetic engineering experiments such as DNA recombination technology laid the foundation for the generation of animal transgenic technology. In the past 50 years, with the continuous deepening of research on transgenic technology of model organisms, more and more new methods have been invented, such as microinjection, electroporation, retroviral vector, liposome transfection, sperm Vector method, somatic cell nuclear transfer method, etc. [0003] my country has entered the international frontier in the field of marine biological genomes. However, the functional gene verification technology centered on gene editing tech...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/89
CPCC12N15/89
Inventor 连姗姗包振民王师胡晓丽赵亮杨祖晶朱晓梅汪星磊
Owner OCEAN UNIV OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com