The invention provides a new application of nitro-styrene derivatives having a structure shown in a general formula I, and a good effect of inhibiting fructose-1,6-bisphosphatase, with the lowest IC up to 0.36muM. When R1, R2, R3, R4 and R5 are hydrogen, R3 represents hydrogen, methoxyl, hydroxyl, borono, fluorine, chlorine, bromine, amino, phenyl, methyl, ethyl, isopropyl, tert-butyl, trifluoromethyl, amino, cyano, methylthio, benyloxyl, carboxyl or 2-nitroethenyl; when R1 and R2 are hydrogen, R3 is methoxyl, and R5 is nitro, R4 represents hydroxyl or methoxymethanoyl; when R1, R2 and R5 are hydrogen, R3 represents methyoxyl, and R4 represents methyoxyl or hydroxyl; when R1 and R4 are hydrogen and R5 is nitro, R2 and R3 represent hydroxyl; and when R2 is 2-nitroethenyl, R1, R3, R4 and R5 are hydrogen or hydroxyl.