Efficient tea tree gene cytological positioning method
A positioning method and cytology technology, applied in scientific instruments, measuring devices, instruments, etc., can solve the problems of long pretreatment time, long experiment cycle, high operation requirements, etc., achieve short cycle, avoid heterogeneous troubles, and operability and reproducible effects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0034] Mapping of Cytoplasmic Glutamine Synthetase Gene CsGS1.2 in Tea Tree
[0035] Take the annual cutting seedlings of 'Longjing 43' tea tree as the material, take the solution and cultivate it for four weeks, and after the tea seedlings absorb the roots, tender stems, and fully unfolded leaves (see figure 1 ), after embedding and fixing in 5% agarose gel (see figure 2 ), cross-section on a vibrating microtome (VT 1000 S, Leica, Bensheim, Germany), section thickness 50-100 μm, cross-sections of cut roots, stems, and leaves were incubated in PBS buffer at pH 7.4 at room temperature Among them, the CsGS1.2 antibody was prepared by Hangzhou Huaan Biotechnology Co., Ltd., and the rabbit secondary antibody IgG was purchased from Sigma. After hybridization and incubation with the primary antibody and the secondary antibody, it was flatly spread on the glass slide, and after being pressed, the fluorescence was observed and photographed under a 710 two-photon laser confocal micr...
Embodiment 2
[0044] Mapping of Plastid Glutamine Synthetase Gene CsGS2 in Tea Tree
[0045] Take the annual cutting seedlings of 'Longjing 43' tea tree as the material, take the solution and cultivate it for four weeks, and after the tea seedlings absorb the roots, tender stems, and fully unfolded leaves (see figure 1 ), after embedding and fixing in 5% agarose gel (see figure 2), cross-sectioned on a vibrating microtome (VT 1000 S, Leica, Bensheim, Germany), section thickness 50-100 μm, cross-sections of cut roots, stems, and leaves were incubated in 1×PBS (pH 7.4) at room temperature In the buffer solution, the CsGS2 antibody was prepared by Hangzhou Huaan Biotechnology Co., Ltd., and the rabbit secondary antibody IgG was purchased from Sigma. After hybridization and incubation with the primary antibody and the secondary antibody, it was flatly spread on the glass slide, and after being pressed, the fluorescence was observed and photographed under a 710 two-photon laser confocal micro...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More