Environmental protection enzyme produced by bacillus licheniformis BP17 and preparation method thereof

A technology of bacillus licheniformis and environmental protection enzymes, applied in the direction of microorganism-based methods, biochemical equipment and methods, separation methods, etc., can solve the problems of unreported use of bacillus licheniformis, achieve excellent results, improve the breeding environment, reduce Effect of ammonia concentration

Pending Publication Date: 2019-11-05
ANHUI TECH BANK BIO TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The environmentally friendly enzyme products used in the market mainly include bacillus, yeast, lactic acid bacteria, photosynthetic bacteria, etc., and there is no report on the use of Bacillus licheniformis isolated in situ from feces

Method used

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  • Environmental protection enzyme produced by bacillus licheniformis BP17 and preparation method thereof

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preparation example Construction

[0021] In the above-mentioned invention, utilize the preparation method of the environment-friendly ferment that bacillus licheniformis BP17 produces, comprise the following steps:

[0022] Step 1: Prepare liquid medium: take 3g of beef extract, 10g of peptone, 5g of NaCl, 1000ml of distilled water, adjust the pH value to 7.4-7.6, and sterilize at 121°C for 20 minutes by high-pressure steam, and set aside;

[0023] Step 2: Take the strain of Bacillus licheniformis, inoculate it in a liquid medium, and culture it with shaking at 37°C for 12-18 hours to form a seed liquid, and then carry out separate fermentation in a large tank for 48 hours to obtain an environmentally friendly enzyme.

[0024] Wherein, in step 2, fermented Bacillus subtilis and lactic acid bacteria are added before carrying out large tank fermentation and packaging.

[0025] According to the environmental protection enzyme prepared by the above preparation method, the number of viable bacteria is 5.0-10.0x10 ...

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Abstract

The invention discloses an environmental protection enzyme produced by bacillus licheniformis BP17 and a preparation method thereof. Aiming at environmental protection enzyme products used on the market, the environmental protection enzyme mainly includes bacillus subtilis, yeast, lactic acid bacteria, photosynthetic bacteria and the like, and the strain BP17 is separated from pig farm accumulatedmanure, can be proliferated with pig dung and urine as an only nutrient, and is confirmed to be a member of bacillus licheniformis through molecular identification. The bacillus licheniformis BP17 isfermented for 48 h separately and then is mixed with a fermentation product of bacillus subtilis and lactic acid bacteria; and the product is diluted by 10-50 times, then is sprayed on a fecal place,has the effects of deodorization and ammonia reduction, improves the breeding environment, and has the effect superior to similar products on the market.

Description

technical field [0001] The invention relates to the technical field of animal breeding and environmental protection, in particular to an environmentally friendly enzyme produced by using Bacillus licheniformis BP17 and a preparation method thereof. Background technique [0002] Ammonia is a strong irritating gas. According to research reports, the average ammonia emission per head of fattening pigs is 107.18-424.42mg / h, while a pig farm with 10,000 heads per year has an average daily nitrogen emission of 105kg, a large part of which is ammonia (NH 3 ) form into the atmosphere. Excessive ammonia concentration in the pig house will not only affect the growth and development of pigs, but also reduce the body's resistance, thereby inducing various diseases, causing serious economic losses to pig production, and at the same time affecting the breeder and the surrounding environment of the pig farm. There were also hazards. [0003] Pigs living in a low-concentration ammonia en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/58C12R1/125C12R1/07
CPCC12N1/20B01D53/84B01D53/58Y02A50/20
Inventor 张邦辉胡传炯张康薛兵
Owner ANHUI TECH BANK BIO TECH
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