Sf9 cell line free from Sf-RV pollution as well as screening method and application of Sf9 cell line

A screening method and cell line technology, applied in the field of insect cell lines, can solve problems such as concerns about the safety of biological preparations, and achieve the effect of ensuring safety
CN110423726APending Publication Date: 2019-11-08CHANGCHUN ZHUOYI BIOLOGICAL CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHANGCHUN ZHUOYI BIOLOGICAL CO LTD
Publication Date
2019-11-08

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Abstract

The invention relates to an insect cell line and discloses a Sf9 cell line free from Sf-RV pollution as well as a screening method and application of the Sf9 cell line. The cell line is screened fromcommercial Sf9 cells, named as an Sf9-ZY cell line and has the biological collection number of CCTCC NO:C201952. Detection of the proliferation level and protein expression quantity of baculovirus inthe Sf9-ZY cell line indicates that the Sf9-ZY cell line can replace Sf9 cells to serve as host cells of an insect cell-baculovirus expression vector system (BICS) to be applied to expression of recombinant protein for preparing biological products. The Sf9-ZY cell line provided by the invention solves the problem that host cells of the insect cell-baculovirus expression vector system (BICS) are polluted by Sf-RV and guarantees safety of the biological products.
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Description

technical field

[0001] The invention relates to an insect cell line, in particular to a Sf9 cell line without Sf-RV pollution, a screening method and application thereof. Background technique

[0002] In 1969, Vaughn et al. established an insect cell line, which was isolated from the ovary tissue of the pupae of female Spodoptera frugiperda. At that time, 2.6% heat-inactivated insect blood was added to the culture medium. After the establishment, this cell line was provided to Oxford, England. The University's NERC Invertebrate Virology Unit, where this cell line was adapted to medium supplemented with newborn bovine serum instead of medium supplemented with insect blood, at this time the cell line was called IPLB-Sf-21 -AE was later provided to Texas A&M University in the United States, where it was cloned and applied to the protein expression system. The Sf9 cell line was cloned from IPLB-Sf-21-AE by G.E.Smith and C.L.Cherry. It has a more uniform cell shape, is sensitive...

Claims

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