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Construction method of combined animal model of blood stasis, phlegm coagulation syndrome and laryngeal cancer syndrome

A construction method and animal model technology, applied in animal feed, medical formula, additional food elements, etc., can solve problems such as difficult large-scale operation, limitations, single modeling factors, etc., to ensure reliability, repeatability, Good copy effect

Active Publication Date: 2021-10-22
GUANGDONG HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The construction methods of this type of model generally include whole-body freezing method, partial frostbite method, ice water immersion method, wind-cold environment method, ice water immersion plus medicine method, cold stimulation method, cold and cool traditional Chinese medicine method, etc., but there are many shortcomings: 1) There is a lot of randomness in the modeling process, 2) the modeling factors are single or limited, 3) the evaluation method is limited to the observation of symptoms and signs, and blood flow measurement, etc., lacking objective laboratory indicators, 4) due to the lack of objective laboratory indicators, 4) due to the lack of It has immune deficiency and is difficult to tolerate multi-factor intervention, resulting in high animal mortality during modeling, and it is difficult to operate in large quantities. However, most studies on tumor diseases use this type of animal

Method used

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  • Construction method of combined animal model of blood stasis, phlegm coagulation syndrome and laryngeal cancer syndrome
  • Construction method of combined animal model of blood stasis, phlegm coagulation syndrome and laryngeal cancer syndrome
  • Construction method of combined animal model of blood stasis, phlegm coagulation syndrome and laryngeal cancer syndrome

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Embodiment 1

[0029] This example provides a method for constructing an animal model of blood stasis and phlegm coagulation syndrome, which includes the following steps: 3 days after the animal passes the quarantine, it is constructed by a combination of high-fat feed feeding, ice water swimming and epinephrine hydrochloride subcutaneous injection;

[0030] Preferably, the animal is a Balb / c nude mouse, and the high-fat feed contains the following components in mass percentage: 20% sucrose, 15% lard, 1.2% cholesterol, 0.2% sodium cholate, 10% casein, phosphoric acid Hydrogen calcium 0.6%, stone powder 0.4%, premix 0.4% and basic feed 52.2%;

[0031] Preferably, when the animal swims, the temperature of the ice water is 0-4°C, and the animal swims in the ice water until it is unable to raise its head; the abdomen is subcutaneously injected with epinephrine hydrochloride once a day, and the injection amount each time is 0.2mg / 20g (epinephrine hydrochloride and animal weight ratio);

[0032] ...

Embodiment 2

[0043] This embodiment provides a method for constructing a combined animal model of blood stasis and phlegm coagulation syndrome and laryngeal cancer syndrome, which includes the following steps: injecting laryngeal cancer cells under the axilla skin of mice with blood stasis and phlegm coagulation syndrome;

[0044] Preferably, the laryngeal cancer cells are Hep2 laryngeal cancer cells stably expressing luciferase, and the blood stasis and phlegm coagulation syndrome mice are intraperitoneally injected with a luciferase substrate, and the tumor body is photographed fluorescently; the injection amount of the luciferase substrate is 150 mg / kg; laryngeal carcinoma cells at a concentration of 1×10 7 / ml, the injection volume of laryngeal cancer cells is 0.2ml.

[0045] Successfully constructed transplanted tumors on nude mice with "blood stasis and phlegm coagulation syndrome".

[0046] As in Table 2, Figure 5 and Figure 6 As shown, compared with the blank group, the tumor ...

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Abstract

The invention discloses a method for constructing an animal model combining blood stasis and phlegm syndrome and laryngeal cancer syndrome, and belongs to the technical field of animal model construction. Animals with blood stasis and phlegm coagulation syndrome were constructed by combining subcutaneous injection of plain protein; laryngeal cancer cells were injected under the skin of the axilla of animals with blood stasis and phlegm coagulation syndrome, and the animals used were Balb / c nude mice. The construction method of the present invention uses multiple factors to combine blood stasis and phlegm coagulation syndrome with laryngeal cancer, and the verification indexes for successful construction include blood lipid function and coagulation time, and the animal model has high reliability and good repeatability.

Description

technical field [0001] The invention belongs to the technical field of animal model construction, and in particular relates to a method for constructing an animal model combined with syndromes of blood stasis and phlegm coagulation and laryngeal cancer. Background technique [0002] The treatment of laryngeal cancer is still difficult. Traditional Chinese medicine has obvious advantages in the prevention and treatment of laryngeal cancer, but its specific mechanism of action is still unclear. According to the TCM syndrome differentiation and treatment and the combination of four diagnostic methods, blood stasis and phlegm coagulation syndrome are the most basic syndromes for the formation of laryngeal cancer. Therefore, in order to "explore the anti-tumor mechanism of traditional Chinese medicine and realize the modernization of traditional Chinese medicine", it is imminent to construct an animal model combining blood stasis and phlegm syndrome and laryngeal cancer syndrome....

Claims

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Application Information

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IPC IPC(8): A01K67/02A23K20/00A23K20/111A23K20/147A23K20/158A23K20/163A23K20/20A23K20/22A23K20/26A23K50/50A61D7/00A61K31/137
CPCA01K67/02A61D7/00A61K31/137A23K20/00A23K20/111A23K20/147A23K20/158A23K20/163A23K20/20A23K20/22A23K20/26A23K50/50
Inventor 李云英谭希罗秋兰周世卿
Owner GUANGDONG HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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