Unlock instant, AI-driven research and patent intelligence for your innovation.

Extraction method of lentinula edodes (Berk.) Sing polysaccharides

A technology of lentinan and lentinan, which is applied in the field of extraction of lentinan, can solve the problems of inconvenience, complexity, time-consuming sample loss, etc., and achieve the effect of low price, stable properties and strong selectivity

Inactive Publication Date: 2019-11-12
JIANGSU AGRI ANIMAL HUSBANDRY VOCATIONAL COLLEGE
View PDF7 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Sevag method is complex, time-consuming and has a large sample loss. Both the trifluorotrichloroethane method and the trichloroacetic acid method need to be carried out at a relatively low temperature, which is not convenient for the operator.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Extraction method of lentinula edodes (Berk.) Sing polysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0025] A method for extracting lentinan, comprising the following steps:

[0026] S1, material preparation: buy a sufficient amount of dried and fresh shiitake mushrooms in the market, as well as choline chloride, glycerin and K2HPO4, wash and cut the dried and fresh shiitake mushrooms, and store the processed shiitake mushrooms in a fresh-keeping box for low temperature environment It is stored under the environment for subsequent use, and the temperature of the low temperat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an extraction method of lentinula edodes (Berk.) Sing polysaccharides. The extraction method comprises the steps that S1, materials are prepared; S2, lentinula edodes (Berk.) Sing polysaccharide water extraction liquid is prepared; S3, a choline chloride / glycerol deep-eutectic solvent is synthesized; S4, a K2HPO4 solution is prepared; S5, a choline chloride / glycerol-salt double-aqueous-phase system is constructed; and S6, proteins are removed, specifically, choline chloride / glycerol in the step S3, the K2HPO4 solution in the step S4 and the lentinula edodes (Berk.) Singpolysaccharide water extraction liquid in the step S2 are added into a centrifugal tube with scales, then centrifuging treatment is conducted by a centrifuge, and after operation is completed, the centrifugal tube is taken out to wait till two phases are completely separated. The brand new method is adopted to remove the proteins in the lentinula edodes (Berk.) Sing polysaccharides, the deep-eutectic solvent wide in development prospect in recent years and appropriate inorganic salt are adopted to be combined to construct the choline chloride / glycerol deep-eutectic solvent-salt double-aqueous-phase system so as to extract the proteins from the lentinula edodes (Berk.) Sing polysaccharides, the recovery rate of the lentinula edodes (Berk.) Sing polysaccharides is 98.0%, and the removal rate of the proteins of the lentinula edodes (Berk.) Sing polysaccharides is up to 90.8%.

Description

technical field [0001] The invention relates to the technical field of extracting lentinan, in particular to a method for extracting lentinan. Background technique [0002] Mushroom, scientific name Lentinula edodes (Berk.) Sing. Shiitake mushrooms are more unique than other fungi in terms of color, taste, texture, and nutrition. They are popular edible fungi and are in great demand in the global market. [0003] Fresh shiitake mushrooms and dried shiitake mushrooms commonly sold in the market have different tastes. With the development of dehydration technology, shiitake mushrooms are processed into dried shiitake mushrooms, which become a healthy and nutritious snack product. Mushrooms are not only delicious, but also rich in protein, polysaccharides, cellulose, vitamins, amino acids and other nutrients. Shiitake mushrooms can delay aging, inhibit blood pressure rise, fight cancer and tumor, and enhance the body's immune function. [0004] Lentinan is a polar macromolec...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/0024
Inventor 王莹陈毓李锋涛赵丽
Owner JIANGSU AGRI ANIMAL HUSBANDRY VOCATIONAL COLLEGE