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Enzymolysis-membrane filtration circulating device and method for preparing shellfish polypeptides by same

A circulatory device and membrane filtration technology, which can be used in peptide preparation methods, sterilization methods, biochemical equipment and methods, etc., can solve the problems of excessive degradation of substrates, lack of continuity in operation, and low utilization of enzymes. Achieve the effect of improving the conversion rate, improving the utilization rate of raw materials, and good product quality

Pending Publication Date: 2019-11-12
ZHEJIANG WANLI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional enzymatic preparation of polypeptides has problems such as low enzyme utilization, excessive substrate degradation, and non-continuous operation.

Method used

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  • Enzymolysis-membrane filtration circulating device and method for preparing shellfish polypeptides by same

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Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1: a kind of enzymolysis-membrane filtration circulation device, as figure 1 As shown, it includes a raw material pool 1, a first constant temperature tank 2, an enzymatic hydrolysis tank 3, a second constant temperature tank 4, a centrifugal device 5, a membrane filtration module 6, a liquid collection tank 7, a feed pump 8, a first circulation pump 9 and The second circulating pump 10; the raw material pool 1 is arranged in the inside of the first constant temperature tank 2, and the raw material pool 1 is connected with the enzymolysis pool 3 through the feeding pump 8; the enzymolysis pool 3 is arranged in the inside of the second constant temperature tank 4, and the enzymolysis A stirring bar 31 and a pH meter 32 are arranged in the pool 3, and the enzymolysis pool 3 is connected with the inlet of the membrane filter assembly 6 via the centrifugal device 5 and the first circulating pump 9 in sequence; the outlet of the membrane filter assembly 6 is respec...

Embodiment 2

[0025] Embodiment 2: The method for preparing shellfish polypeptides using the enzymolysis-membrane filtration circulation device of Embodiment 1 comprises the following steps:

[0026] (1) Raw material pretreatment: Grind the shellfish meat into minced meat, add 3 times the weight of the minced meat to homogenate with water to obtain a substrate solution with minced shellfish as the substrate, and put it in the raw material of the enzymatic hydrolysis-membrane filtration circulation device In the pool, and the temperature of the first constant temperature tank is controlled at a constant temperature of 40°C;

[0027] (2) Mixed enzyme hydrolysis: control the temperature of the second constant temperature tank at a constant temperature of 40 ℃, pressurize the feed pump, transfer the substrate solution in the raw material pool to the enzymatic hydrolysis pool, and then adjust the pH of the substrate solution value to 7.5, add the mixed enzyme of substrate solution quality 2% in ...

Embodiment 3

[0035] Embodiment 3: The method for preparing shellfish polypeptides using the enzymolysis-membrane filtration circulation device of Embodiment 1 comprises the following steps:

[0036] (1) Raw material pretreatment: Grind the shellfish meat into minced meat, and add 4 times the weight of the minced meat to homogenate to obtain a substrate solution with the minced shellfish as the substrate, which is placed in the raw material of the enzymatic hydrolysis-membrane filtration circulation device In the pool, and the temperature of the first constant temperature bath is controlled at a constant temperature of 50°C;

[0037] (2) Mixed enzyme hydrolysis: control the temperature of the second constant temperature tank at a constant temperature of 50 ℃, pressurize the feed pump, transfer the substrate solution in the raw material pool to the enzymatic hydrolysis pool, and then adjust the pH of the substrate solution When the value reaches 8, add a mixed enzyme with 3% of the mass of t...

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Abstract

The invention discloses an enzymolysis-membrane filtration circulating device and a method for preparing shellfish polypeptides by the same. The enzymolysis-membrane filtration circulating device comprises a raw material tank, a first thermostatic bath, an enzymolysis tank, a second thermostatic bath, a centrifugal device, a membrane filtration assembly, a liquid collecting tank, a feed pump, a first circulating pump and a second circulating pump. The method for preparing shellfish polypeptides by the device comprises steps of pretreatment of raw materials, hydrolysis of mixed enzyme, enzyme deactivation, centrifugation and filtration, membrane filtration and the like. The device can simultaneously and continuously perform hydrolysis of mixed enzyme and membrane filtration and perform enzymolysis again on intercepted ultrafiltrates with large molecular weight, the raw materials are saved, and the utilization rate of the raw materials and the production efficiency of the products are increased. The shellfish polypeptide products prepared by the method have good quality, a large quantity of short polypeptides can be produced, the yield of the shellfish polypeptides can reach 90% or above, meanwhile, the conversion rate of the prepared shellfish polypeptides can be effectively increased to 85% or above, and the inhibition rate of the shellfish polypeptides can be increased to 95%or above.

Description

technical field [0001] The invention relates to the technical field of polypeptide preparation, in particular to an enzymolysis-membrane filtration circulation device and a method for preparing shellfish polypeptide using the device. Background technique [0002] Marine shellfish contain abundant and structurally diverse bioactive peptides. my country's marine shellfish is rich in species, and its output ranks first in the world. In 2014, the output reached 1341.67×10 4 t. Marine shellfish are fat and tender, delicious, and contain a lot of nutrients such as protein, amino acid, and polysaccharides. They are marine organisms with high economic value. In most cases, marine shellfish are only processed into food, with a single variety and low added value. Using enzymatic hydrolysis technology to extract peptides with biological functional activities from marine shellfish or its processing by-products, high-value utilization of marine shellfish resources can obtain better e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/40C12M1/34C12M1/12C12M1/02C12P21/06C07K1/34
CPCC12M21/18C12M27/02C12M29/02C12M41/26C12P21/06C07K1/34
Inventor 吕春霞杨华冯志江张建芳廖慧琦杨留明曹少谦戚向阳张慧恩
Owner ZHEJIANG WANLI UNIV
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