Composition, application, cell and gene-editing pig preparation method for modifying amino acid 561 of CD163 gene

A CD163, gene editing technology, applied in genetically modified cells, cells modified by introducing foreign genetic material, botanical equipment and methods, etc. Strong and wide-ranging effect
CN110438155APending Publication Date: 2019-11-12中农种源(深圳)科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
中农种源(深圳)科技有限公司
Publication Date
2019-11-12

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Abstract

The invention provides a composition, application, cell and gene-editing pig preparation method for modifying an amino acid 561 of a CD163 gene, and relates to the technical field of biology. The composition for modifying the amino acid 561 of the CD163 gene comprises recombinant plasmids and single-stranded donor sequences. The recombinant plasmids comprise gene-editing vector skeletons and a section of DNA sequences connected to the vector skeletons. The single-stranded donor sequences are single-stranded DNA sequences in which the amino acid 561 of the CD163 gene is precisely replaced, theamino acid 561of the CD163 gene is precisely modified, and meanwhile, the situation that the normal expression of remaining amino acids of CD163 gene is destroyed or changed can be avoided; and therefore, on the basis of resisting PRRSV infection, the physiological activity function of CD163 protein is retained to the greatest extent, and the advantages of wide application range and high gene-editing efficiency are achieved.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a composition for modifying the 561st amino acid of the CD163 gene, an application, a cell and a method for preparing a gene-edited pig. Background technique

[0002] Porcine reproductive and respiratory syndrome (PRRS) has the characteristics of high infectivity and strong lethality, and is considered to be one of the most urgent porcine infectious diseases to be overcome. Statistics show that the annual economic loss caused by PRRS in the United States and Europe is as high as 644 million US dollars and 600 million euros.

[0003] CD163 protein is considered to be the key receptor for PRRSV (porcine reproductive and respiratory syndrome virus) to enter cells. Since 2014, several units have successfully produced CD163 gene knockout pigs, and challenge experiments have confirmed that CD163 gene knockout can completely resist PRRSV infection. However, in addition to playing an impor...

Claims

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