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Application for VEGF

A technology of grafts and preparations, applied in the field of biomedicine, can solve problems such as stability attempts and explorations, and achieve the effects of improving the level of treatment, prolonging survival time, and achieving excellent results.

Pending Publication Date: 2019-11-19
SHENZHEN ZHIBO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wang Yongheng et al. have attempted to use growth factors in combination with adipose stem cells (Wang Yongheng et al. Research on the construction of engineered adipose tissue by human adipose stem cells and VEGF-PLGA nanosphere sustained-release system[J].Medical Information.), but Aiming at whether it can promote the growth of microvessels and whether it can promote the differentiation ability of adipose stem cells, it has not further tried and explored its stability in the human body after being mixed with stem cells, and whether all growth factors can be used to interact with No attempt or disclosure has been made on stem cell mixing, whether the mixture has side effects on the human body after the growth factor is mixed with stem cells and other ingredients, and whether the growth factor has any effect on the other ingredients mixed with it.
Moreover, the VEGF in this prior art needs to be made in the form of slow-release VEGF to avoid the problem that it is difficult to continue to function after a large amount of VEGF and adipose stem cells are mixed

Method used

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  • Application for VEGF
  • Application for VEGF

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Preparation of Granular Fat and Adipose Stem Cell "Particle" Mixed Filling

[0021] 1.1 Liposuction: The operating doctor fully communicates with the patient to draw out the area that needs to be filled. Try to be bilaterally symmetrical. If there is another plan for bilateral asymmetry, calculate the approximate total volume of fat needed. Take the inner or outer sides of both thighs as the donor area, use manual syringe 0.04% lidocaine for swelling and anesthesia (avoid too high and too fast pressure), use 2mm non-invasive liposuction needle and 20mL disposable screw syringe for liposuction, liposuction volume double the amount required.

[0022] 1.2 Preparation of granular fat and fat stem cell "particle" mixed filling: After filling the syringe, put the syringe upright and suspend it naturally, then only remove the water in the lower layer of the fat particles in the syringe (if there is little water, you can not exclude it), do not put the suction The l...

Embodiment 2

[0025] Example 2 Initial clinical trial of multi-point, multi-tunnel wet CAL filling

[0026] 2.1 Inclusion criteria

[0027] By adopting multi-point and multi-tunnel methods, from 2017 to 2018, autologous fat grafting was used to fill the wet CAL temporal part in multiple layers. The patient had no other physical abnormalities and agreed to sign the informed consent.

[0028] 2.2 Materials and methods

[0029] 2.2.1 Blank control group: 15 cases, 1 male and 14 females, aged 18-49 years, average 32.3 years old. The operations were performed on the temporal part, or combined with autologous fat in other parts. Among the patients, there were 3 cases of facial traumatic soft tissue depression, 2 cases of facial congenital depression, and 10 cases of temporal depression caused by aging. HGF control group: 15 cases, 2 males and 13 females, aged 19-48 years, with an average of 33.5 years. The operations were performed on the temporal part, or combined with autologous fat in othe...

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PUM

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Abstract

The invention discloses an application for VEGF, specifically to the application of the VEGF in preparation of a graft preparation used for wet CAL. According to the invention, the wet CAL is adoptedto combine with the VEGF to promote vascular synthesis and endothelial cell proliferation, prolong the survival time of transplanted fat, reduce fat necrosis and significantly improve the survival rate and quality of transplanted fat tissue; the absorption rate of fat is reduced; a filling substance tends to be stable; and the treatment level of domestic medical cosmetology is further improved.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to an application of VEGF, in particular to an application of VEGF in preparing a graft preparation for wet CAL. Background technique [0002] Human adipose-derived stem cells (ADSCs) are a kind of adult stem cells discovered by Zuk et al. in 2001 from fat extracted by vacuum liposuction. Like other mesenchymal stem cells (mesenchymalstem cells, MSCs), ADSCs have the potential of self-renewal and multidirectional differentiation. [0003] In recent years, there have been many records of the development of wet adipose stem cell-assisted autologous granule fat filling transplantation, referred to as wet CAL (cell assisted lipotransfer), which directly uses human fat aspirate to stand still, and the lipid and liquid in the syringe are rich in unseparated fresh ADSCs and SVF combined with autologous fat granule transplantation can improve the survival rate of fat cells. However, ...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/38A61L27/50A61L27/54
CPCA61L27/3604A61L27/3834A61L27/50A61L27/54A61L2300/414
Inventor 杨涛杨鸣谦王静贾仕贤
Owner SHENZHEN ZHIBO BIOTECH CO LTD
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