Eurotium cristatum fermentation liquor crude peptide extract and application thereof in keeping freshness of meat products
A technology for the extract of Saccharomyces coronoidis, which is applied in the field of application of the crude peptide extract of the fermented liquid of Saccharomyces coronoidis in the preservation of meat products, which can solve the problem of reducing nutritional value, adverse effects on human health, and reducing the flavor of meat products Characteristics and other issues, to achieve the effect of a broad spectrum of antibacterial
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[0023] Preparation of cooked meat: boil the pork with boiling 25g / L brine for 20 minutes, the ratio of meat to brine is 1:5. Cut cooked meat into uniformly shaped and sized pieces.
[0024] Preparation of the crude peptide extract from the fermentation broth of S. coronoidis:
[0025] (1) Activate Cystis coronoides FZ-4 (CGMCC NO: 17186) in PDA medium, insert bacteria blocks with a diameter of 8 mm in potato dextrose liquid medium, and cultivate under the conditions of 28°C and 200r / min 6d to obtain the culture medium.
[0026] (2) Centrifuge the culture solution obtained in step (1) at 4°C and 13,000 r / min for 20 minutes, use a 0.22 μm filter membrane to remove bacteria, and pass through a 10kDa ultrafiltration membrane to obtain a bacteria-free coronoid Bacteria fermented liquid.
[0027] (3) Pass the Fermented Bacillus coronis obtained in step (2) through DEAE-52 ion exchange resin, the mobile phase is 0.1M Tris-Cl buffer solution with a pH value of 8.1, and use 0.1M NaC...
Embodiment 1
[0034] According to the above steps, put the cooked meat in the treatment group and dip for 20s for fresh-keeping treatment, take it out and drain.
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