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Rapid propagation method for calatheaveitchiana

An arrowroot and beautiful technology, which is applied in the field of rapid propagation of arrowroot, can solve the problems of difficult seed formation, long growth cycle of beautiful arrowroot, and difficulty in quickly multiplying finished products, and achieves improved reproductive efficiency and seedling survival rate. High and improve the effect of traditional system coefficient

Active Publication Date: 2019-11-22
广州市名卉景观科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main reason is that the beautiful arrowroot is difficult to produce seeds, and ordinary growers can only reproduce by division. The growth cycle of the beautiful arrowroot is long, and it is difficult to quickly reproduce a large number of finished products

Method used

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  • Rapid propagation method for calatheaveitchiana

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for fast breeding of beautiful arrowroot, comprising:

[0030] S11. Take the female parent of the beautiful arrowroot, tube culture after disinfection, take the side buds, peel off part of the leaf sheaths, soak them in alcohol after cleaning, then soak them in disinfectant solution for disinfection, take them out and clean them after a period of time, peel off part of the leaf sheaths, and soak them again until Disinfectant solution, after a period of time, take it out for cleaning, peel off the remaining leaf sheaths, and obtain explants;

[0031] S12. Inducing and culturing adventitious buds on the explants until obtaining adventitious bud seedlings;

[0032] S13. Take the adventitious bud seedlings, divide them into individual plants, remove the leaves, keep the stem section, carry out adventitious bud proliferation and culture on the stem section, until 2~3 adventitious bud seedlings re-grow from the stem section;

[0033] S14. Repeat S13 for a certain nu...

Embodiment 2

[0038] The difference between Example 2 and Example 1 is that in the step S11, the female parent of Arrowroot is taken and disinfected by irrigation with chlorothalonil solution, and the chlorothalonil solution is chlorothalonil: solvent=1:2000 The dilution solution; the tube culture is to transplant the female parent to a clean greenhouse for cultivation, the controlled environment temperature is 15~17°C, the humidity is 80~95%, and the light intensity is not more than 10000lux; the length of the side buds is 2~5cm , in the ultra-clean workbench, peel off part of the leaf sheath, keep the innermost 2~3 layers of clean leaf sheath, cut the incision flat with a scalpel, soak in clean water and detergent for 5 minutes, rinse with clean water, wash with 75% alcohol Soak for 30s, then immerse in 0.6% sodium hypochlorite solution for disinfection for 10-12 minutes, stir continuously during the disinfection process; take out the sterilized side buds and wash them with water, peel off...

Embodiment 3

[0040] The difference between Example 3 and Example 1 is that in the S12 step, when the explants are induced to cultivate adventitious buds, the ambient temperature is controlled at 25-26°C, the light duration is 10h / d, the light intensity is 2000-3000lux, and the culture is carried out for 60 ~80d.

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Abstract

The invention relates to the field of plant growing, and provides a rapid propagation method for calatheaveitchiana. The method is used for improving the propagation efficiency of the calatheaveitchiana. The rapid propagation method for the calatheaveitchiana comprises the steps that a calatheaveitchiana female parent is taken, a lateral bud is taken, part of a leaf sheath is stripped, and then the lateral bud is cleaned, then soaked with ethyl alcohol and immersed into a disinfection solution for disinfection; part of the leaf sheath is stripped, and the lateral bud is immersed into the disinfection solution again for a time and then taken out to be cleaned; the remaining leaf sheath is stripped to obtain an explant; the explant is subjected to adventitious bud induction culture until anadventitious bud seedling is obtained; the adventitious bud seedling is taken, the stem section is obtained and subjected to adventitious bud multiplication culture, rooting induction culture is conducted until a complete plant tissue culture seedling with main roots is obtained; the tissue culture seedling is cleaned and then transplanted into the substrate, and a lot of calatheaveitchiana seedlings are obtained. In the culture process, the seedling survival rate is high, more calatheaveitchiana plants can be obtained within the same culture time, and the propagation efficiency is greatly improved.

Description

technical field [0001] The invention relates to the technical field, in particular to a method for rapid breeding of arrowroot. Background technique [0002] Beautiful arrowroot (scientific name: Calatheaveitchiana ) is a perennial herbaceous foliage plant of the Arrowroot family Arrowroot. Native to Ecuador and Peru. It likes a warm, humid, and semi-shady growth environment. It is afraid of dryness and avoids strong light exposure. The suitable growth temperature is 22-28°C. It is an excellent indoor shade-loving foliage plant. Used to decorate bedrooms, living rooms, offices and other places. Although there is large-scale production in China, it fails to meet the great demand of the market for beautiful arrowroot. The main reason is that Arrowroot is difficult to produce seeds, and ordinary growers can only reproduce by division. The growth cycle of Arrowroot is long, and it is difficult to quickly reproduce a large number of finished products. [0003] Tissue culture...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 何文杰陈慧余结梅郑慈真江碧玉马文卿
Owner 广州市名卉景观科技发展有限公司
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