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Preparation method and application of purple flower holly leaf extract

An extract, the technology of Sijiqing, applied in the field of preparation of Sijiqing extract, can solve the problems of high toxicity, difficult to remove, and difficult to obtain effects, and achieve the effect of enhanced lipophilicity and improved extraction rate

Inactive Publication Date: 2019-11-29
青岛铖泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research and development of therapeutic drugs for viral diseases is relatively slow, mainly due to the strict parasitic nature of the virus. After the virus is infected with the virus, it uses the cellular enzyme system to replicate in the host cell; some viral nucleic acids are integrated in the cell chromosome, which is difficult to remove; internal, making the drug kill the virus and also damage the cells of the body
Existing antiviral chemical drugs are difficult to achieve satisfactory clinical results because of their toxicity or drug resistance
Existing vaccines cannot provide complete protection to pig herds, which has brought great difficulties to its prevention and control. It is urgent to find new prevention and control solutions

Method used

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  • Preparation method and application of purple flower holly leaf extract
  • Preparation method and application of purple flower holly leaf extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation method comprises the following steps:

[0025] S1. Take an appropriate amount of Sijiqing, dry it, crush it, pass through a 10-mesh to 24-mesh sieve, and obtain Sijiqing powder, and set it aside;

[0026] S2. Take Sijiqing powder obtained in step S1 and add an appropriate concentration of acidic ethanol to reflux for extraction for 1.5 hours, and filter, collect medicinal residues and filtrate, and use the filtrate for subsequent use;

[0027] S3, concentrating the filtrate obtained in step S2 to 60°C under reduced pressure and measuring to obtain an extract with a relative density of 1.02;

[0028] S4. Spray-dry the extract obtained in step S3 to obtain Sijiqing extract.

[0029] In the S2 step, 30% ethanol solution containing 0.2% acid was added to 7 times the weight of Sijiqing powder for reflux extraction.

[0030] The acid ethanol in the S2 step is 30% ethanol containing 0.2% acid, and the acid ethanol is 0.2% acid dissolved in 100ml of 30% ethano...

Embodiment 2

[0034] The preparation method comprises the following steps:

[0035] S1. Take an appropriate amount of Sijiqing, dry it, crush it, pass through a 10-mesh to 24-mesh sieve, and obtain Sijiqing powder, and set it aside;

[0036] S2. Take Sijiqing powder obtained in step S1 and add an appropriate concentration of acidic ethanol to reflux for extraction for 2 hours, and filter, collect medicinal residues and filtrate, and use the filtrate for subsequent use;

[0037] S3, concentrating the filtrate obtained in step S2 to 60°C under reduced pressure and measuring to obtain an extract with a relative density of 1.03;

[0038] S4. Spray-dry the extract obtained in step S3 to obtain Sijiqing extract.

[0039] In the S2 step, add a 40% ethanol solution containing 0.3% acid in an amount 8 times the weight of Sijiqing powder for reflux extraction.

[0040] The acid ethanol in the S2 step is 40% ethanol containing 0.3% acid, and the acid ethanol is 0.3% acid dissolved in 100ml of 40% et...

Embodiment 3

[0044] The preparation method comprises the following steps:

[0045] S1. Take an appropriate amount of Sijiqing, dry it, crush it, pass through a 10-mesh to 24-mesh sieve, and obtain Sijiqing powder, and set it aside;

[0046] S2. Take the Sijiqing powder obtained in step S1 and add an appropriate concentration of acidic ethanol to reflux for extraction for 3 hours, and filter, collect medicinal residues and filtrate, and use the filtrate for subsequent use;

[0047] S3, concentrating the filtrate obtained in step S2 to 60°C under reduced pressure and measuring to obtain an extract with a relative density of 1.05;

[0048] S4. Spray-dry the extract obtained in step S3 to obtain Sijiqing extract.

[0049] In the S2 step, 65% ethanol solution containing 0.4% acid was added to 9 times the weight of Sijiqing powder for reflux extraction.

[0050] The acidic ethanol in the S2 step is 65% ethanol containing 0.4% acid, and the acidic ethanol is 0.4% acid dissolved in 100ml of 65% ...

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Abstract

The invention provides a preparation method and application of a purple flower holly leaf extract and belongs to the field of veterinary drugs. According to the technical scheme, the preparation method comprises the following steps: S1, drying a proper amount of purple flower holly leaves, performing crushing, and performing screening till 10-24 meshes so as to obtain purple flower holly leaf powder for later use; S2, adding acidic ethanol of an appropriate concentration into the purple flower holly leaf powder obtained in the step S1, performing refluxing extraction for 1.5-3.0 hours, performing filtering, and collecting medicine dregs and filtrate for later use; S3, performing vacuum concentration on the filtrate obtained in the step S2 till 60 DEG C, and performing measurement so as toobtain an extract of which the relative density is 1.01-1.06; and S4, performing spray drying on the extract obtained in the step S3, so as to obtain the purple flower holly leaf extract. The invention has the beneficial effects that the purple flower holly leaf extract provided by the invention has an inhibition function on porcine pseudorabies virus infection proliferation, and meanwhile, acidicethanol is adopted to implement refluxing extraction for polyphenol components which generally exist in a mode of salts in purple flower holly leaves, so that the extraction rate can be greatly increased.

Description

technical field [0001] The invention relates to the field of veterinary medicine, in particular to a preparation method and application of Sijiqing extract. Background technique [0002] Porcine pseudorabies (Pseudorabies PR) is an acute infectious disease caused by porcine pseudorabies virus (Pseudorabies PRV). The disease is fulminant in pigs. It can cause abortion and stillbirth in pregnant sows, infertility in boars, mass death of newborn piglets, dyspnea and growth stagnation in fattening pigs, etc. It is one of the major infectious diseases that endanger the global pig industry. The research and development of therapeutic drugs for viral diseases is relatively slow, mainly due to the strict parasitic nature of the virus. After the virus is infected with the virus, it uses the cellular enzyme system to replicate in the host cell; some viral nucleic acids are integrated in the cell chromosome, which is difficult to remove; Internally, the drug kills the virus and also ...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61K36/19A61K36/80A61K36/704A61K36/56A61P31/22
CPCA61K36/185A61K36/19A61K36/56A61K36/704A61K36/80A61K2236/333A61K2236/51A61P31/22
Inventor 钟英杰李志明李亮
Owner 青岛铖泰生物科技有限公司
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