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Donkey-derived Salmonella AD19 strain and application thereof

A Salmonella, donkey-derived technology, used in bacteria, antibacterial drugs, veterinary vaccines, etc., can solve the problems of susceptible primiparous dams and foals, and achieve the effects of preventing donkey abortion and improving immunity.

Active Publication Date: 2019-11-29
INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease is very easy to infect primiparous dams and young foals. Most of them have no obvious clinical symptoms before abortion occurs. Abortion occurs suddenly. There are few domestic reports on salmonellosis in equine animals, especially donkeys.

Method used

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  • Donkey-derived Salmonella AD19 strain and application thereof
  • Donkey-derived Salmonella AD19 strain and application thereof
  • Donkey-derived Salmonella AD19 strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1 Materials and methods

[0017] 1.1 Disease data

[0018] In April 2019, a large-scale abortion of female donkeys broke out in a donkey farm in Dong'e, Shandong Province. There were a total of 200 donkeys in this small donkey farm, and 45 female donkeys were aborted, all of which were born at the gestational age of 6-11 months. . Aborted female donkeys showed symptoms such as elevated body temperature and died one after another. Autopsies of two dead donkeys revealed diffuse hemorrhagic inflammation of the cecum and large colon, hepatic fibrosis of the lungs, and necrotic lesions of the kidneys and liver. Tissue samples of mediastinal lymph nodes, heart, lung, kidney, liver, spleen, brain, and colon were collected from a dead foal for bacteriological detection and virus screening.

[0019] 1.2 Main reagents

[0020] Salmonella chromogenic medium, common agar and common nutrient broth medium were purchased from Qingdao Haibo Biological Co., Ltd.; biochemical identif...

Embodiment 2

[0039] 2 Results and analysis

[0040] 2.1 Results of strain culture

[0041] The bacterium is named AD19, and the preservation number (CGMCC No. 18046) is cultured in the Salmonella chromogenic medium. The colony is purple, medium in size, with neat edges, smooth and moist, and round bulges. After the bacterial solution inoculated into the nutrient broth was cultured at 37°C for 24 hours, it was observed by Gram staining that the bacteria were purified and were Gram-negative short bacilli with blunt ends ( figure 1 ).

[0042] 2.2 Biochemical test results

[0043] 2.3 PCR amplification analysis of strain 16S rDNA

[0044]The 16S rDNA PCR amplification product of AD19 strain showed an obvious target band at about 1500bp ( figure 2 ). The 16S rDNA PCR sequence of the isolate was carried out, and the sequence was compared by Blast. The results showed that the 16S rDNA sequence of the isolate was Salmonella.

[0045] 2.4 Results of strain pathogenicity test

[0046] 2.4....

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Abstract

The invention belongs to the field of biotechnology, and particularly relates to a donkey-derived Salmonella AD19 strain and application thereof. The microbial preservation number is CGMCC N0.18046. The preservation date is June 27, 2019. The preservation unit is: China General Microbiological Culture Collection Center. According to the invention, a gram-negative bacillus is isolated and screenedfrom dead foal disease material collected from a donkey farm in Shandong Province. After purified and cultured, the isolated strain was identified as Salmonella by morphological identification, biochemical identification and cloning and sequence analysis of 16SrDNA. The results of pathogenicity tests show that the Salmonella strain has certain lethality to mice and most notably can cause abortionof pregnant mice. This strain can be used to prepare an inactivated vaccine of Salmonella causing donkey abortion. The inactivated vaccine prepared from the strain has good immunogenicity, high safetyand good protection effects.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a donkey Salmonella AD19 strain and an application thereof. Background technique [0002] Salmonellosis is one of the zoonotic diseases of great significance in public health. The pathogenic Salmonella belongs to the Enterobacteriaceae family, including those bacteria that cause food poisoning, gastroenteritis, typhoid fever and paratyphoid fever. In addition to infecting humans, they can also infect many animals including mammals and birds. Salmonellosis is a general term for livestock and poultry infectious diseases caused by bacteria of the genus Salmonella. This disease often occurs in pigs, chickens, cattle and other animals. Among them, Salmonella is the pathogen that causes paratyphoid fever in horses, and it is related to abortion and neonatal sepsis. associated with polyarthritis. The disease is very easy to infect primiparous dams and foals. Most of them have ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K39/112A61P31/04C12R1/42
CPCA61K39/0275A61K2039/521A61K2039/552A61P31/04C12N1/205C12R2001/42
Inventor 张伟张燕朱曼玲齐鹏飞孙海涛朱荣生黄迪海王怀中王长法
Owner INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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