Targeted delivery carrier and application of small peptide for stroke protection across the blood-brain barrier
A blood-brain barrier and targeted delivery technology, applied in non-active ingredients medical preparations, active ingredients-containing medical preparations, peptide/protein ingredients, etc., can solve problems such as diagnosis and treatment and treatment obstacles, and achieve sensitive repeatability , Product use effect is good, low cost effect
Active Publication Date: 2021-08-03
SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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Problems solved by technology
Our study found that HSP27 can exert a significant protective effect on cerebral ischemia by directly inhibiting ASK1, but the blood-brain barrier is a major obstacle in the diagnosis and treatment of HSP27
Method used
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Embodiment 1
[0041] Example 1, this example provides the synthesis steps and effect evaluation of gold-coated silicon nanomaterials.
[0042] (1) Preparation process
[0043] SiO 2The surface is aminated with APTES, and then prepared and adsorbed 2-3 nm gold nanoparticles to form composite particles. Hydrogen peroxide is used as a reducing agent to catalyze on the surface of the composite material, and the chloroauric acid is continuously reduced to make it continuously deposited on the surface of the composite material. SiO 2 surface, thereby forming a certain thickness and covering a complete gold nano-coating layer. After the preparation, centrifuge at 3000 rpm for 10 min, discard the supernatant, collect the precipitate, and resuspend (OD 700 nm =1.0). Characterization of nanomaterials see figure 2 .
[0044] Synthesis of drug release carrier: prepare 5,5-dithiodinitrobenzoic acid solution (DTNB) with different concentrations, take out 300 μL and add to 1 mL (OD 700 nm =1.0) in...
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Abstract
The present invention relates to a small peptide cross-blood-brain barrier targeted delivery and release carrier system for stroke protection, comprising a composite nanoparticle carrier coupled with Raman signal molecules and small peptides containing transmembrane proteins to form a multi-membrane state Drug delivery system across the blood-brain barrier. To provide the preparation and application of a targeted drug release carrier system across the blood-brain barrier based on composite nanomaterials to achieve targeted and controlled release of drugs in the brain. At the same time, the drug release process can be monitored through SERS detection and fluorescence images. Improve treatment efficiency. Nanoprobes have the advantages of low cost, rapidity, simplicity, sensitivity and good repeatability. Effective use of the small characteristics of nanomaterials and the micro-region resolution characteristics of micro-Raman spectrometers enables real-time detection of pharmaceutical reagents at trace levels. 4. The method of the present invention is stable and reliable, and the product is effective in use.
Description
technical field [0001] The invention belongs to the field of biosensing and targeted drug release and delivery. The invention relates to the construction and application of a nanometer drug release carrier, in particular to a targeted delivery release carrier and application of a small peptide for cerebral apoplexy protection across the blood-brain barrier. Background technique [0002] Stroke has the characteristics of high morbidity, high mortality and high disability rate. It is one of the three major causes of human death and the first cause of adult disability worldwide. For the neurological deficits faced by most patients, there is no effective clinical prevention method so far. [0003] HSP27 is a member of the small heat shock protein family HSPB, which can promote cell survival and has complex functions related to inhibiting cell death. Our study found that HSP27 can exert a significant protective effect on cerebral ischemia by directly inhibiting ASK1, but the bl...
Claims
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IPC IPC(8): A61K9/14A61K47/02A61K47/04A61K38/16A61P9/10A61K49/00
CPCA61K9/143A61K38/16A61K49/0002A61K49/001A61K49/0017A61P9/10
Inventor 孙保亮王莹张宗勇毛蕾蕾杨明峰李汉霞王玮琦
Owner SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI