Crossing blood-brain barrier target transmission release carrier for small peptide protection for cerebral apoplexy and application
A technology of blood-brain barrier and targeted delivery, which is applied in the direction of medical preparations with non-active ingredients, medical preparations containing active ingredients, cardiovascular system diseases, etc. It can solve problems such as diagnosis and treatment obstacles, and achieve sensitive repeatability , Good product use effect, huge economic and social benefits
Active Publication Date: 2019-12-03
SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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Problems solved by technology
Our study found that HSP27 can exert a significant protective effect on cerebral ischemia by directly inhibiting ASK1, but the blood-brain barrier is a major obstacle in the diagnosis and treatment of HSP27
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Embodiment 1
[0041] Embodiment 1. This embodiment provides the synthesis steps and effect evaluation of the gold-coated silicon nanomaterial.
[0042] (1) Preparation process
[0043] SiO 2 The surface is modified with APTES for amination, and then 2-3 nm gold nanoparticles are prepared and adsorbed to form composite particles. Hydrogen peroxide is used as a reducing agent to catalyze the surface of the composite material to continuously reduce chloroauric acid to continuously deposit it on the surface. SiO 2 The surface, thereby forming a certain thickness and covering a complete gold nano-coating layer. After the preparation, centrifuge at 3000 rpm for 10 min, discard the supernatant, collect the precipitate, and resuspend (OD 700 nm =1.0). The characterization of nanomaterials see figure 2 .
[0044] Synthesis of drug release carrier: configure 5,5-dithiodinitrobenzoic acid solution (DTNB) of different concentrations, take out 300μL and add to 1mL (OD 700 nm =1.0) in the gold-coated sil...
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Abstract
The invention relates to a crossing blood-brain barrier target transmission release carrier system for small peptide protection for cerebral apoplexy. The crossing blood-brain barrier target transmission release carrier system comprises a composite nanometer particle carrier, and is used for coupling Raman signal molecules and small peptide containing transmembrane protein to form a multi-membranecrossing blood-brain barrier medicine release system. The invention provides preparation and an application of the crossing blood-brain barrier target transmission release carrier system based on a composite nanometer material, so that targeted and controllable release of medicines in brains can be realized. Besides, through SERS detection and photofluorogram, the medicine release process can bemonitored, and the treatment efficiency can be improved. A nanometer probe has the advantages of being low in cost, quick, simple, sensitive, good in repeatability and the like. The characteristic that the nanometer material is small in size and the microdifference characteristic of a micro-Raman spectroscopy are effectively used, so that the micro level of medicine reagents can be detected in a real time manner. The method is stable and reliable, and the product is good in using effect.
Description
Technical field [0001] The invention belongs to the field of biosensing and drug targeted release delivery. The present invention relates to the construction and application of a nanometer drug release carrier, in particular to a delivery carrier and application of a targeted delivery across the blood-brain barrier of small peptides for stroke protection. Background technique [0002] Stroke has the characteristics of high morbidity, high mortality, and high disability rate. It is one of the three major causes of death in humans and the first cause of disability in adults worldwide. As for the neurological deficits faced by most patients, effective clinical prevention and treatment methods are lacking so far. [0003] HSP27 is a member of the HSPB family of small heat shock proteins, which can promote cell survival and has complex functions related to inhibiting cell death. Our study found that HSP27 can exert a significant protective effect on cerebral ischemia by directly inhib...
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IPC IPC(8): A61K9/14A61K47/02A61K47/04A61K38/16A61P9/10A61K49/00
CPCA61K9/143A61K38/16A61K49/0002A61K49/001A61K49/0017A61P9/10
Inventor 孙保亮王莹张宗勇毛蕾蕾杨明峰李汉霞王玮琦
Owner SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI