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Method for purifying plant anthocyanin by using magnetic nano material

A technology of magnetic nano and magnetic nano-carriers, applied in chemical instruments and methods, alkali metal compounds, alkali metal oxides/hydroxides, etc., can solve the problems of low stability and bioavailability of anthocyanins that are not suitable for large-scale use , limited wide and effective application and other issues, to achieve the effect of easy industrial production and large-scale application, easy separation and repeated use, and targeted and controlled release

Inactive Publication Date: 2020-06-26
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the relatively low stability and bioavailability of anthocyanins are the main obstacles limiting their widespread and effective applications
[0003] 中国专利CN101525327B、CN103145681B、CN103420970B(25)、CN104961783B、CN105175382B、CN106632204A、CN106632208A、CN107793456A、CN109400569A、CN110183410A等均分别公开了利用大孔吸附树脂对花青素进行分离纯化的方法,该方法有机残留物含量 High, the separation effect is poor, it needs to be used in conjunction with other equipment, the operation process is complicated, and the macroporous resin needs to be rinsed and regenerated before it can be used repeatedly, which is time-consuming and laborious; Chinese patents CN106749151A and CN106923136A disclose methods for extracting anthocyanins using ultra-high pressure devices. This method has a large investment in high-voltage equipment and consumes a large amount of electric energy, and is not suitable for large-scale use; Chinese patents CN106674186A and CN108658923A respectively use citric acid solution and acidic oxidation potential water as extractants to purify anthocyanins. This method will introduce additives, resulting in residual

Method used

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  • Method for purifying plant anthocyanin by using magnetic nano material
  • Method for purifying plant anthocyanin by using magnetic nano material
  • Method for purifying plant anthocyanin by using magnetic nano material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] FeCl 3 6H 2 O is added to deionized water with an iron ion concentration of 0.05mol / L, stirred at 20°C and 650r / min, and after dissolving, add trisodium citrate dihydrate and urea at a concentration of 0.05mol / L and 0.10mol respectively / L, stir at 350r / min, add dopamine and polyethyleneimine after dissolving, accounting for 12.3% of the total mass of the solute, stir at 800r / min, add NH with a concentration of 25% to 28% after dissolving 3 h 2 O solution, adjust the pH to 8, and continue to stir for 15 minutes to make it fully mixed. The above mixture was transferred to an autoclave and reacted at 130°C for 3h. After the reaction, cool to room temperature, remove the supernatant, transfer the precipitated solid to a centrifuge tube, wash with deionized water and absolute ethanol three times, ultrasonically disperse at a speed of 5000r / min, centrifuge for 5min, and remove the supernatant liquid to remove impurities. The obtained solid was dried in a vacuum drying...

Embodiment 2

[0038] FeCl 3 6H 2 O, FeCl 2 4H 2O is added to deionized water with an iron ion concentration of 0.8mol / L, stirred at 30°C and 800r / min, and after being dissolved, add trisodium citrate dihydrate and urea at a concentration of 0.10mol / L and 0.15mol respectively / L, stir at 500r / min, add polyacrylamide and polyethylene glycol after dissolving, accounting for 18.5% of the total mass of the solute, stir at 1500r / min, add the concentration of 25% to 28% after dissolving NH 3 h 2 O solution, adjust the pH to 10, and continue to stir for 25 minutes to make it fully mixed. The above mixture was transferred to an autoclave and reacted at 180°C for 12h. After the reaction, cool to room temperature, remove the supernatant, transfer the precipitated solid to a centrifuge tube, wash with deionized water and absolute ethanol 5 times, ultrasonically disperse at 8000r / min, centrifuge for 10min, remove the supernatant liquid to remove impurities. The obtained solid was dried in a v...

Embodiment 3

[0042] FeCl 2 4H 2 O, FeSO 4 7H 2 O is added to deionized water with an iron ion concentration of 1.2mol / L, stirred at 40°C and 1000r / min, and after dissolving, add trisodium citrate dihydrate and urea at a concentration of 0.20mol / L and 0.25mol respectively / L, stir at 600r / min, add acrylic resin after dissolving, accounting for 28.5% of the total mass of the solute, stir at 2000r / min, add NH with a concentration of 25% to 28% after dissolving 3 h 2 O solution, adjust the pH to 11, and continue to stir for 45 minutes to make it fully mixed. The above mixture was transferred to an autoclave and reacted at 240°C for 20h. After the reaction, cool to room temperature, remove the supernatant, transfer the precipitated solid to a centrifuge tube, wash with deionized water and absolute ethanol for 6 times, ultrasonically disperse at a speed of 12000r / min, centrifuge for 15min, and remove the supernatant liquid to remove impurities. The obtained solid was dried in a vacuum ...

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Abstract

The invention discloses a method for purifying plant anthocyanin by using a magnetic nano material. The magnetic nano material prepared by the method is controllable in particle size of 10-600nm, andcan effectively adsorb pigments and enhance the stability of pigment. The magnetic nano material is used as an adsorption carrier, separation and repeated use are easy, targeted controllable release is achieved, the purification and separation efficiency is high, the method is simple, and the method can be suitable for adsorbing plant cyanine of different structures.

Description

technical field [0001] The invention relates to the technical field of application of biological nanometer carrier materials and plant purification, in particular to a method for purifying plant anthocyanins by using magnetic nanomaterials. Background technique [0002] Anthocyanins are hydrophilic and water-soluble polyphenolic plant pigments and metabolites, and are one of the important antioxidants. They have a wide range of pharmacological properties, such as antioxidant, anti-aging, anti-inflammatory, antibacterial, and anticancer. Anthocyanins have a strong antibacterial effect, such as inhibiting the growth of Listeria monocytogenes, Staphylococcus aureus, Salmonella enteritidis and Vibrio parahaemolyticus. Anthocyanins can lower blood sugar levels by protecting B cells, improving insulin resistance, increasing insulin secretion, improving liver function and inhibiting carbohydrate hydrolase. In addition, anthocyanins have the ability to induce the apoptosis of HepG...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/28B01J20/30C07D311/62
CPCB01J20/08B01J20/261B01J20/262B01J20/28009C07D311/62
Inventor 蒋希芝徐磊夏礼如严旎娜冯敏汪敏柳军皮杰
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES