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A kind of sodium alginate/penetrating peptide/plasmid ternary nanocomposite vaccine and its preparation and application

A nanocomposite and sodium alginate technology, which is applied in the direction of DNA/RNA vaccination, vaccines, non-effective ingredients of polymer compounds, etc., can solve problems such as the difficulty of oral vaccine research, achieve inhibition of tumor growth, facilitate industrial production, The effect of uniform and stable particle size

Active Publication Date: 2021-04-30
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, different particle surface properties are required to penetrate the mucus layer and the intestinal epithelial cell layer, which poses difficulties for the study of oral vaccines

Method used

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  • A kind of sodium alginate/penetrating peptide/plasmid ternary nanocomposite vaccine and its preparation and application
  • A kind of sodium alginate/penetrating peptide/plasmid ternary nanocomposite vaccine and its preparation and application
  • A kind of sodium alginate/penetrating peptide/plasmid ternary nanocomposite vaccine and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Weigh 1 mg of the membrane-penetrating peptide Tat (amino acid sequence RKKRRQRR) and dissolve it in 2 mL of purified water, and dissolve evenly to prepare a 0.5 mg / mL aqueous solution of the membrane-penetrating peptide Tat. Under magnetic stirring, slowly drop 40 μL of 0.5 mg / mL aqueous solution of the plasmid encoding human pre-melanoma melanoma protein gp100 antigen (sequence shown in SEQ ID NO: 5) into the aqueous solution of membrane-penetrating peptide Tat, and magnetically stir at room temperature for five Minutes to obtain the penetrating peptide / plasmid binary nanocomplex for later use; wherein, the mass ratio of the penetrating peptide to the plasmid is 50:1. Dissolve 5 mg of sodium alginate (viscosity 200 mPa·s, 1% sol, mass percent, 20° C.) in 2 mL of purified water, and dissolve evenly to prepare a 2.5 mg / mL sodium alginate aqueous solution. Under magnetic stirring, the sodium alginate aqueous solution was slowly added dropwise to the above-mentioned membr...

Embodiment 2

[0044] Weigh 1 mg of the membrane-penetrating peptide Tat (amino acid sequence RKKRRQRR) and dissolve it in 2 mL of purified water, and dissolve evenly to prepare a 0.5 mg / mL aqueous solution of the membrane-penetrating peptide Tat. Under magnetic stirring, slowly drop 40 μL of 0.5 mg / mL aqueous solution of the plasmid encoding human pre-melanoma melanoma protein gp100 antigen (sequence shown in SEQ ID NO: 5) into the aqueous solution of membrane-penetrating peptide Tat, and magnetically stir at room temperature for five Minutes to obtain the penetrating peptide / plasmid binary nanocomplex for later use; wherein, the mass ratio of the penetrating peptide to the plasmid is 50:1. Dissolve 2 mg of sodium alginate (viscosity 200 mPa·s) in 2 mL of purified water, and dissolve uniformly to prepare a 1 mg / mL sodium alginate aqueous solution. Under magnetic stirring, the sodium alginate aqueous solution was slowly added dropwise to the above-mentioned membrane-penetrating peptide / plasm...

Embodiment 3

[0046] Weigh 1 mg of the membrane-penetrating peptide Tat (amino acid sequence RKKRRQRR) and dissolve it in 2 mL of purified water, and dissolve evenly to prepare a 0.5 mg / mL aqueous solution of the membrane-penetrating peptide Tat. Under magnetic stirring, slowly drop 40 μL of 0.5 mg / mL aqueous solution of the plasmid encoding human pre-melanoma melanoma protein gp100 antigen (sequence shown in SEQ ID NO: 5) into the aqueous solution of membrane-penetrating peptide Tat, and magnetically stir at room temperature for five Minutes to obtain the penetrating peptide / plasmid binary nanocomplex for later use; wherein, the mass ratio of the penetrating peptide to the plasmid is 50:1. Dissolve 1 mg of sodium alginate (viscosity: 200 mPa·s) in 2 mL of purified water, and dissolve uniformly to prepare a 0.5 mg / mL sodium alginate aqueous solution. Under magnetic stirring, the sodium alginate aqueous solution was slowly added dropwise to the above-mentioned membrane-penetrating peptide / pl...

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Abstract

The invention discloses a sodium alginate / penetrating peptide / plasmid ternary nanocomposite vaccine and its preparation and application. The ternary nanocomposite vaccine is composed of sodium alginate, membrane-penetrating peptide and plasmid, wherein the mass ratio of sodium alginate to membrane-penetrating peptide is 1‑5:1, and the mass ratio of membrane-penetrating peptide to plasmid is 5‑400 :1; it can be used to prepare plasmid oral drugs, and provides a new idea for oral delivery of plasmids. The preparation method is to obtain a sodium alginate / membrane penetrating peptide / plasmid ternary nanocomposite vaccine through electrostatic adsorption-mediated layer-by-layer assembly. The preparation conditions are mild, the preparation process is easy to control, the preparation method is simple, and the particle size of the obtained complex is uniform and stable. , is conducive to industrial production.

Description

technical field [0001] The invention relates to the technical field of biomedical complexes, in particular to a sodium alginate / membrane-penetrating peptide / plasmid ternary nanocomposite vaccine and its preparation and application. Background technique [0002] Cancer is the second leading cause of death for humans worldwide. In recent years, a variety of immune methods based on tumor vaccines have been studied, such as antigen-specific tumor vaccines, dendritic cell vaccines, and whole-cell vaccines, all of which are promising treatments (Experimental dermatology.2001; 10 :143-154.). Although cell-based vaccines are currently the main form of tumor vaccines in clinical trials, antigen-specific vaccines can generate an immune response against specific tumor antigens, thereby providing a more precise anti-tumor immune response. A few tumor-associated antigens have been expressed. Bits were identified and entered the clinical trial stage, such as the conserved expression of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/00A61K9/50A61K47/36A61K47/42A61P35/00
CPCA61K9/5036A61K39/0011A61K47/42A61K2039/53A61K2039/542A61P35/00
Inventor 邱利焱沈雨润
Owner ZHEJIANG UNIV
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