Preparation method of polyclonal antibody for specific recognition of dynein regulatory complex protein 1(DRC1) and application of preparation method

Abstract

The invention relates to the technical fields of bioscience and a detection reagent, and particularly discloses a preparation method of a polyclonal antibody for specific recognition of dynein regulatory complex protein 1(DRC1). The preparation method comprises the following steps of (1) analyzing protein DRC1 epitope: through epitope analysis, selecting a target fragment as recombinant expressionof the protein DRC1, wherein the gene sequence of the target fragment is shown as SEQID N0.1 in a sequence table; (2) after PCR amplification of DNA of the selected target fragment, inserting the DNAinto an expression vector, constructing a recombinant expression vector containing the nucleotide sequence as shown in SEQID NO.1, transforming the recombinant expression vector to escherichia coli competence cells to obtain recombination protein DRC1 antigen; and (3) performing animal immunization with the recombination protein DRC1 antigen, and through serum, obtaining the polyclonal antibody for specific recognition of the protein DRC1. During preparation of the recombination protein, an escherichia coli expression system is adopted, so that the expression of the recombination protein canbe well guaranteed, the expression yield is high, and large-batch preparation of the polyclonal antibody for specific recognition of the protein DRC1 is facilitated.

Description

technical field [0001] The invention relates to the technical fields of biological science and detection reagents. Background technique [0002] DRC1 protein (Dynein regulatory complex protein 1, Dynein regulatory complex protein 1) is expressed by the DRC1 gene. DRC1 is involved in encoding the central component of the nexin-dynein regulatory complex (nexin-dynein regulatory complex, N-DRC), which regulates the assembly of ciliary dynein. DRC1 co-assembles with DRC2 to form the N-DRC central scaffold and its connection to the outer double-tubular microtubules. Mutations in this gene can cause movement disorders of cilia. Studies have shown that the abnormal movement of cilia is closely related to various respiratory diseases, such as sinusitis, COPD, cystic fibrosis (cysticfibrosis), primary ciliary dyskinesia (PCD, Primary ciliary dyskinesia), etc. (References: Jing, J.C.and J.J.Chen, et al. (2017). "Visualization and Detection of Ciliary Beating Pattern and Frequency i...

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Problems solved by technology

[0005] However, the relevant research on the preparation method of the protein DRC1 polyclonal antibody in the prior art is still in a blank stage

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