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Method for establishing early screening scale for risk assessment of gestational diabetes

A technology for gestational diabetes and risk assessment, which is applied in biochemical equipment and methods, microbiological measurement/inspection, health index calculation, etc., can solve the problems of high false negative rate and inconsistency of genes, and achieve good accuracy, Improved perinatal outcome, good correlation

Active Publication Date: 2019-12-03
北京天平永达生物科技发展有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still deficiencies: (1) This document was published in 2013, and more research on GDM has appeared in the past 6 years. The SNP sites covered by this research are not enough to fully explain the pathogenesis of GDM , has a high false negative rate, and many new GDM-related susceptibility loci have been excavated in 6 years; (2) A large amount of analysis data in this literature is the cohort data of the European population, Caucasian The genetic difference with the yellow race will lead to inconsistent conclusions for the selected genes in the Chinese cohort

Method used

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  • Method for establishing early screening scale for risk assessment of gestational diabetes
  • Method for establishing early screening scale for risk assessment of gestational diabetes
  • Method for establishing early screening scale for risk assessment of gestational diabetes

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Experimental program
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Effect test

Embodiment 1

[0026] Through the review of authoritative scientific research literature at home and abroad, first determine the selection criteria for the susceptibility gene SNP:

[0027] 1. If there is a case-control analysis of GWAS in Chinese cohort studies, and there are significant differences, the impact of sample size on data value should also be considered;

[0028] 2. There is a case-control analysis of GWAS in Europeans, and there are significant differences, and it has been verified in a Chinese cohort study;

[0029] 3. The case-control analysis of the candidate gene method in the multi-center study of Chinese people, there is a significant difference, and the results are consistent;

[0030] 4. The results of gene functional studies in the Chinese population are consistent, and it is related to the occurrence of gestational diabetes.

[0031] Through the above four criteria, a total of 28 candidate gene loci were found as shown in Table 1:

[0032] Table 1 Candidate gene loc...

Embodiment 2

[0036] Design primers for nucleic acid mass spectrometry analysis of each SNP site, perform PCR amplification and gene detection;

[0037] The primer design for nucleic acid mass spectrometry analysis of each SNP site is shown in Table 2:

[0038] Table 2 Primers for nucleic acid mass spectrometry analysis of each SNP site

[0039]

[0040]

[0041]

[0042] To save detection costs, set the reaction system to 5 μL:

[0043] Reaction system for PCR amplification (5μL)

[0044] reaction system:

[0045] Template DNA 1 μL,

[0046] Primer mix 1μL,

[0047] 10×Buffer 0.5μL,

[0048] MgCl2 (25mM) 0.4μL,

[0049] dNTP (25mM) 0.1 μL,

[0050] Hotstar (5U / μL),

[0051] ddH2O 1.8 μL;

[0052] Reaction conditions: Place the sealed 384-well plate on an ABI9700 PCR instrument for reaction: pre-denaturation at 95°C for 2min, (95°C for 30s, 56°C for 30s, 72°C for 1min) for 45 cycles, 72°C for 5min, Hold 4°C.

Embodiment 3

[0054] To establish a risk assessment model for complex diseases, first establish the role of each gene locus in the occurrence of the disease through the case-control analysis data in the literature that meets the requirements (need to meet: original data, high article quality rating, Chinese cohort data). Weights, expressed as OR values.

[0055] Each locus has 3 genotypes aa, ab and bb. The OR values ​​of the three genotypes are ORa, ORab, ORb, respectively. The genotype frequencies of each genotype in Asians are Pa, Pab, Pb, respectively. Then the genetic risk value of the disease can be calculated by the following formula:

[0056] RI=(Pa1*ORa1+Pb1*ORb1+Pab1*ORab1)*(Pa2*ORa2+Pb2*ORb2+Pab2*ORab2)*...*(Pa28*ORa28+Pb28*ORb28+Pab1*ORab28)

[0057] The individual's genetic risk value is PI=OR1*OR2*...*OR28 / RI.

[0058] The PI value is compared with the prevalence rate of the population, the lifetime risk of the subject is the multiple of the population level. If it exceed...

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Abstract

The invention discloses a method for establishing an early screening scale for risk assessment of gestational diabetes. The method comprises the following steps of consulting domestic and foreign authoritative scientific research literature, determining a susceptibility gene SNP selection standard, and finding a candidate gene site; designing a primer for nucleic acid mass spectrometric analysis on each SNP sites, and performing PCR amplification and genetic test; establishing a risk assessment model of complex diseases, and calculating heredity risk value of individuals; and combining the heredity risk valve with an external cause risk factor, and establishing the early screening scale for risk assessment of gestational diabetes. According to the method, internal and external factor conjoint analysis is adopted, and through the early screening scale for risk assessment of gestational diabetes, early screening is performed, the risk assessment accuracy is greatly increased, and the clinical diagnosis fail rate is reduced.

Description

technical field [0001] The invention relates to the technical field of gestational diabetes risk assessment, in particular to a method for establishing an early screening scale for gestational diabetes risk assessment. Background technique [0002] The significance of the establishment of early screening methods for gestational diabetes is as follows: (1) The prevalence of gestational diabetes is high, which is very harmful to pregnant women, fetuses, newborns, and postpartum women; (2) Clinically, it will not be diagnosed until 24 to 28 weeks Whether you have GDM or not, you have missed the best time for sugar control; some studies have shown that only using high-risk factor screening, even if there are no high-risk factors, 34.7% of people are still diagnosed with gestational diabetes in the second and third trimesters , indicating that the early screening method has a high rate of missed diagnosis, so there is no particularly effective early screening method in clinical p...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883G16B20/20G16B25/20G16H50/30
CPCC12Q1/6883G16B20/20G16B25/20G16H50/30C12Q2600/156Y02A90/10
Inventor 张影
Owner 北京天平永达生物科技发展有限公司
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