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Algal protein peptide with ACE inhibition and antioxidant functions and preparation method of algal protein peptide

An algal protein and antioxidant technology, which is applied in the field of marine food processing to achieve the effects of good activity inhibition, good functional properties and low ash content

Active Publication Date: 2019-12-06
ANHUI GUOTAI BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the evaluation of antioxidation and ACE inhibitory activity of active peptides derived from algae protein obtained by enzymatic hydrolysis.

Method used

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  • Algal protein peptide with ACE inhibition and antioxidant functions and preparation method of algal protein peptide
  • Algal protein peptide with ACE inhibition and antioxidant functions and preparation method of algal protein peptide
  • Algal protein peptide with ACE inhibition and antioxidant functions and preparation method of algal protein peptide

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preparation example Construction

[0033] An algal protein peptide with ACE inhibitory and antioxidant functions, characterized in that it includes: the amino acid sequence is LEILDGDIVR, SLIDLLK, GDFGISVGR, and the amino acid sequence is LEILDGDIVR, SLIDLLK, GDFGISVGR peptides account for 60%-68% of the total. The preparation method of described protein peptide comprises the following steps:

[0034] Step 1: Prepare Arthrospira protein liquid; select Arthrospira powder, place the Arthrospira powder in water that meets the sanitary standards for drinking water, and the ratio of Arthrospira powder to solvent water is 5:1-8:1.

[0035] The above mixed solution is heated to 95-100° C., stirred for 30-60 minutes, and the Arthrospira powder is completely dissolved to obtain an Arthrospira solution. The obtained Arthrospira solution is placed, cooled to 65-75° C., put into a high-speed shearing machine, and processed for 2-3 minutes to obtain Arthrospira protein liquid.

[0036] Step2: protein modification; adjust t...

Embodiment 1

[0049] Step1: Prepare Arthrospira protein liquid; select 50g of Arthrospira powder, place the Arthrospira powder in water that meets the sanitary standards for drinking water, heat the above mixed solution to 95°C, stir for 30 minutes, and let the Arthrospira powder completely Dissolve to obtain Arthrospira solution. Place the obtained Arthrospira solution, cool it down to 65°C, put it into a high-speed shearing machine, and process it for 2 minutes to obtain Arthrospira protein solution.

[0050] The ratio of the Arthrospira powder to solvent water is 5:1.

[0051] Step2: protein modification; adjust the temperature of the Arthrospira protein solution obtained in Step1 to 70°C, and use an ultrasonic generator to perform ultrasonic treatment for 20 minutes to change the tissue structure of the Arthrospira protein. Regulate the protein content in the Arthrospira protein liquid to make it 7%.

[0052] The frequency of the ultrasonic wave is 60KHz.

[0053] Step3: the first st...

Embodiment 2

[0063] Step1: Prepare Arthrospira protein liquid; select 50g of Arthrospira powder, place the Arthrospira powder in water that meets the sanitary standards for drinking water, heat the above mixed solution to 100°C, and stir for 60 minutes to let the Arthrospira powder completely Dissolve to obtain Arthrospira solution. The obtained Arthrospira solution was placed, cooled to 75° C., put into a high-speed shearing machine, and treated for 3 minutes to obtain Arthrospira protein liquid.

[0064] The ratio of the Arthrospira powder to solvent water is 8:1.

[0065] Step2: protein modification; adjust the temperature of the Arthrospira protein liquid obtained in Step1 to 75°C, and use an ultrasonic generator to conduct ultrasonic treatment for 30 minutes to change the tissue structure of the Arthrospira protein. Regulate the protein content in the Arthrospira protein solution to make it 10%.

[0066] The frequency of the ultrasonic wave is 90KHz.

[0067] Step3: the first step ...

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Abstract

The invention relates to an algal protein peptide with ACE inhibition and antioxidant functions and a preparation method of the algal protein peptide. An amino acid sequence of the algal protein peptide with the ACE inhibition and antioxidant functions is LEILDGDIVR, SLIDLLK, and GDFGIVSVGR, and peptides with the amino acid sequence of LEILDGDIVR, SLIDLLK, and GDFGIVSVGR account for 60% to 68% ofthe total amount. The preparation method of the algal protein peptide with the ACE inhibition and antioxidant functions is characterized in that the preparation method comprises the following steps that 1, an arthrospira protein solution is prepared; 2, protein allosteric is conducted; 3, first step enzymolysis is conducted; 4, second step enzymolysis is conducted; 5, a separation solution is prepared; 6, secondary ultrafiltration separation of protein peptides is conducted; 7, first elution is conducted; 8, second elution is conducted; and 9, constituents of a second section of spirulina powder are determined

Description

technical field [0001] The invention relates to the technical field of marine food processing, in particular to an algal protein peptide with ACE inhibition and anti-oxidation functions and a preparation method thereof. Background technique [0002] Hypertension is a typical cardiometabolic disorder and an important cause of diseases such as atherosclerosis, myocardial infarction, cerebral hemorrhage and renal failure. According to the report of the American Cardiovascular Health Association, as of 2018, there are more than 1 billion hypertensive patients in the world, so hypertension has become a global health problem. The accumulation of oxidative free radicals produced by the body's metabolism can damage endothelial cells, induce vascular inflammation and cause blood vessel blockage, resulting in abnormally high blood pressure. Disturbance of the renin-angiotensin-aldosterone system that regulates blood pressure is also an important cause of hypertension. Angiotensin-co...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12P21/06C07K1/36C07K1/34C07K1/20C07K1/16C07K1/14A61P9/12A61P39/06
CPCA61P9/12A61P39/06C07K7/06C12P21/06
Inventor 张恒罗永康刘怀高于海波
Owner ANHUI GUOTAI BIOTECHNOLOGY CO LTD
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