Method for supercritical carbon dioxide fluid extraction of sterol-rich sea-buckthorn seed oil

Abstract

The invention relates to the technical field of phytosterol extraction, particularly to a method for supercritical carbon dioxide fluid extraction of sterol-rich sea-buckthorn seed oil. The method comprises the following steps: drying and smashing sea-buckthorn seeds, sieving the smashed sea-buckthorn seeds, weighing sea-buckthorn seed powder samples, putting the sea-buckthorn seed powder samplesinto an extraction kettle, and carrying out supercritical carbon dioxide fluid extraction to obtain sea-buckthorn seed oil rich in sea-buckthorn sterol. According to the invention, the smashed sea-buckthorn seed powder particles can be in full contact with an extraction medium in the process of supercritical carbon dioxide extraction; the extraction process of the sea-buckthorn seed oil is explored through single-factor experiments, and then optimization is conducted through a response surface experiment; the extraction yield of the sea-buckthorn sterol in the sea-buckthorn seed oil is improved, an extraction period is shortened, and extraction efficiency is improved; the whole extraction process is simple and suitable for industrialization; and when the sterol obtained through extractionis developed into food, health-care products, additives and the like, further purification is not needed, oil with high fat solubility does not need for dissolution or compounding, cost is low, and remarkable economic benefits can be obtained.

Description

technical field [0001] The invention relates to the technical field of plant oil sterol extraction, in particular to a method for extracting sterol-rich seabuckthorn seed oil with a supercritical carbon dioxide fluid. Background technique [0002] Seabuckthorn (Hippophae rhamnoides Linn.) is a shrub or small tree of the genus Acidiaceae, also known as Vinegar Willow, Blackthorn, Acidthorn, Qicharigana (Mongolian name), Dapu (Tibetan name), Jihan (Uyghur name). It is mainly distributed in the vast areas of Eurasia. It has the characteristics of cold and drought resistance, salt and alkali resistance, barrenness resistance, and wide ecological adaptability. The fruit of the seabuckthorn tree is called seabuckthorn fruit, the juice of seabuckthorn can be used as a beverage, the pulp of seabuckthorn can be used to separate fruit oil, and the seeds of seabuckthorn can be used to produce seabuckthorn seed oil. Seabuckthorn fruit is also a health care product and medicine with gr...

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Problems solved by technology

[0004] The extraction of seabuckthorn seed oil is the pre-order step of seabuckthorn phytosterol extraction. At present, there are few extraction and purification methods for seabuckthorn oil in seabuckthorn seeds. There are four main extraction methods: (1) Pressing method: all mechanical rolling, the obtained Oil can retain the original natural characteristics, but the oil yield is low, and it is mostly used for raw material pretreatment, not suitable for mass production

(2) Carbon dioxide extraction process: It is a production process adopted by large edible oil production enterprises in China. The advantage is that there is no solvent residue. The disadvantage is that the equipment cost is high and the production cost is high. It is not suitable for the production of edible oil with small output. , especially the production of raw material oils for health products such as seabuckthorn seed oil

(3) Solvent extraction process: It is a widely used vegetable oil extraction process. The advantages are low equipment cost, low production cost, and high oil yield. The disadvantage is that it is difficult to control solvent residues.

(4) Grease permeation and diffusion method: use vegetable oils such as sunflower oil as a solvent to extract seabuckthorn seed oil, but the dryness of vegetable oil is much larger than that of organic solvents, the penetration and diffusion speed is low, and the extraction efficiency is poor

This method has the following disadvantages: (1) the extraction process is complex, not suitable for industrialization, and the cost is high; (2) because organic solvents are used in the extraction process, too many organic solvents are introduced, and if the sterols obtained by extraction are to be developed into food , health products, additives, etc., further purification is required, which virtually increases the cost; (3) due to the high fat solubility of seabuckthorn sterols, if the purified sterols are to be developed into food, health products, etc., it is also necessary to select fat Only by dissolving or compounding oils with high solubility can they be better developed into corresponding products, which also increases the process of product preparation and increases production costs

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