Method for extracting preserved bacteria solution from faeces

Abstract

The invention relates to a method for extracting a preserved bacteria solution from faeces. The method comprises the following steps that (1) a faecal bacteria solution is prepared; (2) a freeze-drying protecting agent is added into the faecal bacteria solution obtained in the step (1), then the faecal bacteria solution is subjected to freeze drying, and thus faecal bacteria freeze-dried powder isobtained; (3) the faecal bacteria freeze-dried powder obtained in the step (2) is placed in a refrigerator at -80 DEG C to be preserved; and (4) when the faecal bacteria freeze-dried powder obtainedin the step (3) is taken and used, the faecal bacteria freeze-dried powder is restored to be liquid and then is taken and used. Compared with the prior art, according to the method, microflora is concentrated and can be preserved at the low temperature for a long term, the survival rate of faecal bacteria after being preserved for one week reaches 85.24%, microbiota transplantation is facilitatedthrough the mode of the faecal bacteria freeze-dried powder, and transplantation can be directly conducted through an oral administration feeding method.

Description

technical field [0001] The invention belongs to the technical field of feces and bacteria transplantation, and in particular relates to a method for extracting and preserving bacteria liquid from feces. Background technique [0002] Fecal microbiota transplantation (FMT), also known as fecal microbiota therapy or intestinal microbiota transplantation, is to transplant the functional flora in the feces of healthy people into the gastrointestinal tract of patients to rebuild the intestinal flora with normal functions and achieve intestinal Diagnosis and treatment of intestinal and extraintestinal diseases. At present, fecal microbiota transplantation has been regarded as a special organ transplantation for the treatment of Clostridium difficile infection, inflammatory bowel disease, irritable bowel syndrome, metabolic syndrome, neurodevelopmental dysplasia and neurodegenerative diseases , autoimmune enteropathy, intestinal food allergy, etc. Modern fecal bacteria transplanta...

AI Technical Summary

Problems solved by technology

Modern fecal bacteria transplantation technology is mainly the method of instrument intervention, such as gastroscopy, gastric tube, colonoscopy, jejunal tube, etc. Treatment of diarrhea-type IBS and ulcerative colitis requires multiple transplants, and each time the fecal flora needs to pass through the catheter After passing through the stomach and duodenum, it is injected into the distal end of the duodenum. The process is complicated, the patient has certain pain, and the application is limited.

The prepared fecal bacteria samples are stored at room temperature for a short time and need to be transplanted immediately. Although they can be frozen at -80'C after adding sterile glycerin, it is inconvenient to use