Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for acquiring transgenic antibacterial peptide chlorella mediated by magnetic nanoparticles

A technology of magnetic nanoparticles and chlorella, applied in the field of transgenics, can solve problems such as limitations, and achieve the effects of high targeting, convenient operation, and fast transfection speed

Active Publication Date: 2019-12-06
BEIJING AGRO BIOTECH RES CENT
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the continuous deepening of the research on Chlorella transgenic technology, there have been many reports on the successful transformation of Chlorella through gene gun method, Agrobacterium infection method, electric shock transformation method, PEG-mediated method, etc. The researchers successfully reported Genes or functional exogenous genes are transformed into Chlorella, and expression is achieved, but there are certain limitations in the above methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for acquiring transgenic antibacterial peptide chlorella mediated by magnetic nanoparticles
  • Method for acquiring transgenic antibacterial peptide chlorella mediated by magnetic nanoparticles
  • Method for acquiring transgenic antibacterial peptide chlorella mediated by magnetic nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0037] 1 Materials and methods

[0038] 1.1 Experimental materials

[0039] 1.1.1 Algae and bacteria

[0040] Chlorella Ellipsoidea was purchased and purified from the freshwater algae bank of the Institute of Hydrobiology, Chinese Academy of Sciences; the strain was Escherichia Coli DH5α strain.

[0041] 1.1.2 Reagents and instruments

[0042]Magnetic nanoparticle polyMAG200 (the magnetic nanoparticle polyMAG200 is ferric oxide magnetic nanoparticle with a diameter of 100nm), the magnetic plate was purchased from Chemicell Company, the reagents used for the preparation of the medium and solution were purchased from Sinopharm Group, and the antibiotics were purchased from Shanghai Baisai Biotechnology Co., Ltd., plasmid extraction kits were purchased from Tiangen Biochemical Technology Co., Ltd., cellulase, hemicellulose, and pectinase were purchased from Suleibao Technology Co., Ltd., and other chemical reagents were domestic analytical grade.

[0043] 1.1.3 Medium prepara...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for acquiring transgenic antibacterial peptide chlorella mediated by magnetic nanoparticles. The method comprises the following steps: (1) constructing an antibacterial peptide expression vector; (2) screening sensitivity of chlorella to G418; and (3) carrying out magnetic nanoparticle mediated chlorella transformation: carrying out pretreatment by taking chlorellacells as receptors, carrying out magnetic nanoparticle-gene construction, carrying out magnetic transformation on the chlorella cells, and subjecting transformed chlorella to screening, subculture and transformation so as to obtain transgenic chlorella. The method for acquiring transgenic antibacterial peptide chlorella mediated by magnetic nanoparticles is high in transfection speed, high in efficiency and convenient to operate.

Description

technical field [0001] The invention relates to the field of transgenes, in particular to a method for obtaining antimicrobial peptide chlorella mediated by magnetic nanoparticles. Background technique [0002] As a short peptide substance derived from natural organisms, antimicrobial peptides widely exist in animals, plants and microorganisms. More than a thousand kinds of antimicrobial peptides have been discovered so far. After continuous and in-depth research, it is found that a variety of antimicrobial peptides usually have the characteristics of short peptide chains and good thermal stability. Antimicrobial peptides have a different metabolism and mechanism of action from traditional antibiotics, exhibit broad-spectrum antibacterial properties against bacteria, fungi, viruses, etc., and hardly produce drug resistance. As the most potential active substance that is expected to replace antibiotics at present, and has characteristics that antibiotics do not have, the st...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/82C12N15/62C12R1/89
CPCC07K14/43563C07K2319/02C07K2319/036C12N15/8206
Inventor 张秀海薛静曹苏珊陈绪清杨凤萍张铭芳杜运鹏高俊莲
Owner BEIJING AGRO BIOTECH RES CENT
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com