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Process of crudely extracting SBTI proteins by taking soybean whey as raw material based on soybean deep processing waste liquid

A technology of clear water and protein, applied in the field of bioengineering, can solve the problems of resource waste, environmental pollution, failure to achieve resource reuse and environmental protection purposes, and achieve the effect of reducing microbial load, reducing relative proportion, and increasing protein concentration

Pending Publication Date: 2019-12-10
HEFEI TIANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies of the prior art, the present invention provides a process for crudely extracting SBTI protein based on soybean deep-processing waste liquid and using bean clear water as raw material, which solves the waste of resources caused by the discharge of existing bean clear wastewater and cannot be achieved by developing a protein The extraction method is used to extract and utilize the soybean trypsin inhibitor in the soybean clear wastewater well, and the recycling of the wastewater cannot be achieved, so as to save the production cost of the soybean trypsin inhibitor, and the avoidance of direct discharge of the soybean clear water to the In the external environment, pollution to the environment and waste of resources occur, thus failing to achieve the purpose of resource reuse and environmental protection

Method used

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  • Process of crudely extracting SBTI proteins by taking soybean whey as raw material based on soybean deep processing waste liquid

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Experimental program
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Effect test

Embodiment 1

[0027] S1. Soy water pretreatment: Fresh bean water is rich in sources, with a pH of about 4.5 and a temperature of about 40°C. Because it contains a large number of microorganisms, and contains sugars, proteins, and inorganic salts, if it is not treated in time, it will soon become rancid . Therefore, after the soy clear water is received, it must be pretreated immediately. First, the residual insoluble matter in the soy clear water is removed by physical means to obtain clear pretreated soy clear water. The microbial load has been significantly reduced; for the smooth operation of the subsequent process, it is very necessary to ensure the quality of the crude extract. The physical means to remove insolubles in soy water are disc centrifuge clarification, 1 micron filter bag filtration clarification and 250kDa hollow fiber membrane method Combined process method of filtration and clarification;

[0028] S2. Membrane concentration: use ultrafiltration membrane to concentrate,...

Embodiment 2

[0035] S1. Soy water pretreatment: Fresh bean water is rich in sources, with a pH of about 4.5 and a temperature of about 40°C. Because it contains a large number of microorganisms, and contains sugars, proteins, and inorganic salts, if it is not treated in time, it will soon become rancid . Therefore, after the soy clear water is received, it must be pretreated immediately. First, the residual insoluble matter in the soy clear water is removed by physical means to obtain clear pretreated soy clear water. The microbial load is significantly reduced; for the smooth operation of the subsequent process, it is very necessary to ensure the quality of the crude extract, and the physical means to remove the insoluble matter in the soy water is the clarification process of the disc centrifuge;

[0036] S2. Membrane concentration: use ultrafiltration membrane to concentrate, moderately increase protein concentration, reduce the relative proportion of oligosaccharides, and the molecular...

Embodiment 3

[0043] S1. Soy water pretreatment: Fresh bean water is rich in sources, with a pH of about 4.5 and a temperature of about 40°C. Because it contains a large number of microorganisms, and contains sugars, proteins, and inorganic salts, if it is not treated in time, it will soon become rancid . Therefore, after the soy clear water is received, it must be pretreated immediately. First, the residual insoluble matter in the soy clear water is removed by physical means to obtain clear pretreated soy clear water. The microbial load is significantly reduced; for the smooth operation of the subsequent process, it is very necessary to ensure the quality of the crude extract. The physical means to remove insoluble matter in soybean water is the 1 micron filter bag filtration clarification process;

[0044] S2. Membrane concentration: use ultrafiltration membrane to concentrate, moderately increase protein concentration, reduce the relative proportion of oligosaccharides, and the molecular...

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Abstract

The invention discloses a process of crudely extracting SBTI proteins by taking soybean whey as a raw material based on soybean deep processing waste liquid. The process concretely comprises the following steps of S1, pretreatment of soybean whey; S2, membrane concentration; S3, protein precipitation with salting out; S4, separation of salting-out precipitates; S5, salting-out precipitate dissolution; S6, membrane concentration and spray drying; and S7, storage and transportation of ASP as a crude extract. The invention relates to the technical field of bioengineering. For the process of crudely extracting the SBTI proteins by taking the soybean whey as the raw material based on the soybean deep processing waste liquid, by developing a protein extraction method, a soybean trypsin inhibitorin soybean whey wastewater can be well extracted and utilized to well achieve the purpose of recycling the wastewater to save the production cost of the soybean trypsin inhibitor, the situation thatthe soybean whey is directly discharged into an external environment to cause environment pollution and resource waste is avoided, resource waste cannot be caused, and the purposes of resource reutilization and environmental protection are achieved.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a process for crudely extracting SBTI protein based on soybean deep processing waste liquid and using soybean clear water as raw material. Background technique [0002] Soy Bean Trypsin Inhibitors (English Soy Bean Trypsin Inhibitors, referred to as "SBTI") is a protein pharmaceutical excipient obtained through natural extraction and refinement from soybeans. The SBTI mainly includes two proteins, namely BBI (Bowman-Birk Inhibitor , molecular weight is about 8kDa) and KTI (Kunitz trypsin inhibitor, molecular weight is about 21kDa), the former can inhibit trypsin activity, the latter can inhibit trypsin and chymotrypsin activity, in SBTI products, BBI protein and KTI Proteins are composed in certain proportions. [0003] Soybean water waste liquid In non-genetically modified soybean deep-processing food enterprises, the "alkali-dissolved acid precipitation" process is gene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/81C07K1/30C07K1/34C07K1/36
CPCC07K14/8114C07K14/81Y02P60/87
Inventor 吕荟孙长生杨俊朋袁夏清柏冠军
Owner HEFEI TIANHUI BIOTECH CO LTD
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