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Preparation method of improved mesenchymal stem cells and application thereof

An improved technology of mesenchymal stem cells, applied in the field of biopharmaceuticals, can solve the problems of lower homing rate of MSCs and affect the treatment effect, and achieve the effect of promoting directional migration and improving the treatment effect

Active Publication Date: 2019-12-13
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been confirmed in multiple liver injury treatment models that the reduced homing rate of MSCs largely affects its therapeutic effect

Method used

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  • Preparation method of improved mesenchymal stem cells and application thereof
  • Preparation method of improved mesenchymal stem cells and application thereof
  • Preparation method of improved mesenchymal stem cells and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0025] Sources of materials used in the examples are explained:

[0026] 6-8 week-old SPF-grade C57BL / 6 was purchased from the Institute of Model Animals, Nanjing University;

[0027] Human umbilical cord MSCs were provided by Tianjin Angsai;

[0028] Flow antibodies were purchased from BioLegend;

[0029] Dir lipid dye was purchased from Thermo Fisher Scientific, the Transwell chamber was purchased from Corning (Corning), and the human source FSTL1 recombinant protein was purchased from R&D Company;

[0030] Human MSCs-specific medium was purchased from R&D Company, and other cell culture reagents were purchased from Life Technology Company.

[0031] Preparation and in vitro characterization of the improved MSC of the present invention.

[0032] 37°C 5% CO 2 Human umbilical cord MSCs (containing 1‰ penicillin and 1‰ streptomycin) were used in the incubator, and when the confluence reached 90%, they were subcultured at a ratio of 1:2, and continued to be cultured until the...

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Abstract

The invention discloses a preparation method of mesenchymal stem cells and an application thereof. The method disclosed by the invention comprises the step of culturing the mesenchymal stem cells by adopting a culture medium added with the follistatin protein 1. The MSCs cell product obtained by the preparation method disclosed by the invention can be applied to treatment of inflammation and fibrosis of liver and other organs. Meanwhile, the preparation method provided by the invention can promote directional migration of the MSCs to damaged organs in vivo, and improves the homing efficiency and immunomodulatory ability of the MSCs.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and relates to a preparation method and application of mesenchymal stem cells (MSCs). Background technique [0002] MSCs are a kind of mesoderm-derived non-hematopoietic adult stem cells, which have strong self-renewal ability and can differentiate into mesoderm-derived chondrocytes, bone cells, adipocytes and various ectoderm- and endoderm-derived cells. MSCs exist in almost all tissues, and can be isolated from bone marrow, adipose tissue, liver, muscle, lung and other tissues and expanded in vitro. MSCs from different tissue sources generally have similar biological characteristics: adherent growth, expression of specific surface markers CD73, CD90, and CD105, and generally no expression of hematopoietic-related surface markers such as CD14, CD34, and CD45 (except for adipose-derived MSCs that express CD34), which can be induced into osteoblasts, chondrocytes and adipocytes in vitr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A61K35/28A61P29/00A61P1/16
CPCC12N5/0662A61K35/28A61P29/00A61P1/16C12N2501/16
Inventor 韩英郑小红周霞王敬博周新民郭长存
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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