Preparation method of improved mesenchymal stem cells and application thereof

An improved technology of mesenchymal stem cells, applied in the field of biopharmaceuticals, can solve the problems of lower homing rate of MSCs and affect the treatment effect, and achieve the effect of promoting directional migration and improving the treatment effect
CN110564679AActive Publication Date: 2019-12-13FOURTH MILITARY MEDICAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
FOURTH MILITARY MEDICAL UNIVERSITY
Publication Date
2019-12-13

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Abstract

The invention discloses a preparation method of mesenchymal stem cells and an application thereof. The method disclosed by the invention comprises the step of culturing the mesenchymal stem cells by adopting a culture medium added with the follistatin protein 1. The MSCs cell product obtained by the preparation method disclosed by the invention can be applied to treatment of inflammation and fibrosis of liver and other organs. Meanwhile, the preparation method provided by the invention can promote directional migration of the MSCs to damaged organs in vivo, and improves the homing efficiency and immunomodulatory ability of the MSCs.
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Description

technical field

[0001] The invention belongs to the technical field of biopharmaceuticals, and relates to a preparation method and application of mesenchymal stem cells (MSCs). Background technique

[0002] MSCs are a kind of mesoderm-derived non-hematopoietic adult stem cells, which have strong self-renewal ability and can differentiate into mesoderm-derived chondrocytes, bone cells, adipocytes and various ectoderm- and endoderm-derived cells. MSCs exist in almost all tissues, and can be isolated from bone marrow, adipose tissue, liver, muscle, lung and other tissues and expanded in vitro. MSCs from different tissue sources generally have similar biological characteristics: adherent growth, expression of specific surface markers CD73, CD90, and CD105, and generally no expression of hematopoietic-related surface markers such as CD14, CD34, and CD45 (except for adipose-derived MSCs that express CD34), which can be induced into osteoblasts, chondrocytes and adipocytes in vitr...

Claims

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