A cartilage-bone repair scaffold with bionic gradient and its preparation method

Abstract

The invention discloses a cartilage-bone repair support with bionic gradient and a preparation method thereof. For the cartilage-bone repair support, a cartilage layer of an interwoven porous structure is prepared from sodium hyaluronate and chitosan, a subchondral bone layer of a porous structure is prepared from graphene oxide, chitosan and nano-hydroxyapatite, and structure, composition and function bionics of natural articular cartilage is realized by combining a coprecipitation method and a gradient refrigeration technology by referring to an in-situ bionic thorough. The obtained supporthas an upper layer of structure and a lower layer of structure, nano-hydroxyapatite presents change of bionic gradient distribution which is gradually increased from the cartilage layer to the subchondral bone layer; meanwhile, the cartilage layer in the upper layer is of a mutually penetrating interwoven hierarchical pore structure, the subchondral bone layer is of an array microtube structure which is axially arranged and is vertically through, the support material is excellent in mechanical performance, strong in interface bonding property and good in biological performance, and is expectedto become a novel composite material for treating cartilage-bone defect.

Description

technical field [0001] The invention belongs to the field of composite materials, and in particular relates to a cartilage-bone repair bracket with a bionic gradient and a preparation method thereof. Background technique [0002] Articular cartilage damage caused by trauma, disease, degeneration and other factors is a common orthopedic disease in clinical practice. Especially in the face of the aging trend of the global population, articular cartilage damage is particularly prominent. The country has brought enormous mental pressure and a heavy economic burden. Because articular chondrocytes are cells in the terminal stage of differentiation, without blood vessels, lymphatic vessels and nerves, and lack of corresponding nerve and humoral regulation, the self-repair ability of cartilage defects is limited. On the basis of traditional tissue engineering, the development of in situ tissue engineering technology provides a new opportunity for cartilage repair and functional rec...

AI Technical Summary

Problems solved by technology

For example, the invention patent with the application number 201810059754.2 discloses a biomimetic oriented cartilage scaffold released by two-factor programming. The two parts of the upper surface layer and the lower transitional layer prepared separately are bonded by silk fibroin to form a complete scaffold. , found that it has a good effect on promoting proliferation and differentiation of BMSCs; however, the upper and lower layers of scaffolds are combined to form an overall scaffold, which makes the interface binding force poor; the invention patent with patent number 201410397070.5 announced the integration of bionic interface structure Cartilage-bone scaffold, which is a 1:1 mixture of silk fibroin solution and hydroxyapatite aqueous solution, the main inorganic component of human bone, to prepare a calcified layer and a subchondral bone layer. The two parts of the bone bracket are well connected, which solves the problem of weak bonding between the bracket layer and the layer interface to a certain extent.

However, this method of artificially quantitatively designing the combination of hydroxyapatite particles and organic polymers directly mixed cannot simulate the continuous gradient distribution characteristics of the inorganic components of natural articular cartilage.

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