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A biomimetic heterogeneous integrated bone-cartilage repair scaffold and preparation method thereof

A cartilage repair and calcified cartilage technology, applied in the direction of prosthesis, tissue regeneration, medical science, etc., can solve the problems of lack of multi-level structure and weak interface bonding force, and achieve the effect of promoting stability and enhancing the mechanical properties of organic matter

Active Publication Date: 2021-06-15
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the invention patent with the application number of 201810059754.2 discloses a biomimetic oriented osteochondral scaffold released by two-factor programming. The upper surface layer and the lower transitional layer prepared separately are bonded by silk fibroin to form a complete scaffold; however, the upper and lower two Part of the overall scaffold formed by combination, the interface bonding force is weak; and there is no complete multi-level structure of osteochondral integration at the simulated joint, especially the subchondral bone structure

Method used

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  • A biomimetic heterogeneous integrated bone-cartilage repair scaffold and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0053] 1. Preparation of Subchondral Bone Layer Precursors

[0054] (1) Dissolve 1.00 g of chitosan in an acetic acid solution with a volume fraction of 2%, and stir magnetically for 30 min under a water bath at 37°C to obtain a chitosan acid solution;

[0055] (2) 8 mL each of 2 mol / L calcium nitrate solution and 1.2 mol / L dipotassium hydrogen phosphate solution were added to the mixed solution obtained in step (1) in turn, and fully stirred to make them evenly mixed to obtain a subchondral bone layer complex Precursor solution; where the Ca / P molar ratio is n(Ca 2+ ):n(PO 4 3- ) = 1.67:1;

[0056] (3) injecting the homogeneous mixture of chitosan and inorganic ions prepared in (2) into a mold, and placing it in a refrigerator at -20°C to freeze to form solid A;

[0057] 2. Preparation of precursor complexes of calcified cartilage layer and subchondral bone layer

[0058] (4) Dissolving 0.75 g of chitosan in an acetic acid solution with a volume fraction of 2%, stirring ...

Embodiment 2

[0071] 1. Preparation of Subchondral Bone Layer Precursors

[0072] (1) Dissolve 1.00 g of chitosan in an acetic acid solution with a volume fraction of 2%, and stir magnetically for 30 min under a water bath at 37°C to obtain a chitosan acid solution;

[0073] (2) 8 mL each of 2 mol / L calcium nitrate solution and 1.2 mol / L dipotassium hydrogen phosphate solution were added to the mixed solution obtained in step (1) in turn, and fully stirred to make them evenly mixed to obtain a subchondral bone layer complex Precursor solution; where the Ca / P molar ratio is n(Ca 2+ ):n(PO 4 3- ) = 1.67:1;

[0074] (3) injecting the homogeneous mixture of chitosan and inorganic ions prepared in (2) into a mold, and placing it in a refrigerator at -20°C to freeze to form solid A;

[0075] 2. Preparation of precursor complexes of calcified cartilage layer and subchondral bone layer

[0076] (4) Dissolving 0.75 g of chitosan in an acetic acid solution with a volume fraction of 2%, stirring ...

Embodiment 3

[0089] 1. Preparation of Subchondral Bone Layer Precursors

[0090] (1) Dissolve 1.25 g of chitosan in an acetic acid solution with a volume fraction of 2%, and stir magnetically for 30 min under a water bath at 37°C to obtain a chitosan acid solution;

[0091] (2) 8 mL each of 2 mol / L calcium nitrate solution and 1.2 mol / L dipotassium hydrogen phosphate solution were added to the mixed solution obtained in step (1) in turn, and fully stirred to make them evenly mixed to obtain a subchondral bone layer complex Precursor solution; where the Ca / P molar ratio is n(Ca 2+ ):n(PO 4 3- ) = 1.67:1;

[0092] (3) injecting the homogeneous mixture of chitosan and inorganic ions prepared in (2) into a mold, and placing it in a refrigerator at -20°C to freeze to form solid A;

[0093] 2. Preparation of precursor complexes of calcified cartilage layer and subchondral bone layer

[0094] (4) Dissolve 1.00 g of chitosan in an acetic acid solution with a volume fraction of 2%, and stir ma...

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Abstract

The invention provides a bionic heterogeneous integrated bone-cartilage repair bracket and a preparation method thereof. It is a multi-layer structure bracket material, which includes from top to bottom: a hyaline cartilage layer, made of two biological phases of hyaluronic acid and chitosan Capacitive materials are interwoven to form an interpenetrating porous structure; the calcified cartilage layer is composed of chitosan and nano-hydroxyapatite to form an orientation gradient porous structure; the subchondral bone layer is composed of chitosan and nano-hydroxyphosphorus A homogeneous porous structure formed by hybridization of limestone; an integrated multi-layer repair scaffold material with bone-cartilage heterogeneous structure was constructed by in situ synthesis method combined with directional freezing technology. The composite material synthesized by the invention combines the advantages of each component, has good antibacterial properties, biocompatibility, and the effect of in situ inducing two-way regeneration of bone and cartilage; the bonding force of the interface is enhanced, thereby realizing the integration of the scaffold structure change.

Description

technical field [0001] The invention belongs to the field of composite materials, and in particular relates to a bionic heterogeneous integrated bone-cartilage repair scaffold and a preparation method thereof. Background technique [0002] Articular cartilage damage caused by trauma, disease, degeneration and other factors is a common clinical orthopaedic disease, especially in the face of the aggravation of the global aging trend, the tissue damage at the articular cartilage is particularly prominent, and the expensive medical expenses are caused to the patient's family, The society and the country bring huge mental pressure and heavy economic burden. Because articular cartilage cells are end-stage differentiated cells, and there are no blood vessels, lymphatic vessels and nerves in the articular cartilage, and lack of corresponding nerve and humoral regulation, the self-repairing ability of cartilage defects is limited. Once damaged, the subchondral bone is often involved,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/56A61L27/46A61L27/48A61L27/54
CPCA61L27/46A61L27/48A61L27/54A61L27/56A61L2300/404A61L2430/02A61L2430/06
Inventor 李建树赵耀陈卓鑫丁春梅
Owner SICHUAN UNIV
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