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A kind of hierarchical porous functional scaffold material for mobilizing endogenous neural stem cells to repair spinal cord injury and its preparation method and application

A neural stem cell and scaffold material technology, applied in the field of multi-level porous functional scaffold materials and their preparation, to achieve the effects of promoting motor function recovery, promoting regeneration and repair, and controllable strength

Active Publication Date: 2021-09-21
SHANGHAI TONGJI HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no chitosan-based scaffold material that contains guide holes along the long axis of the material to provide the spatial structure required for neural circuit reconstruction to facilitate the directional migration of endogenous neural stem cells

Method used

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  • A kind of hierarchical porous functional scaffold material for mobilizing endogenous neural stem cells to repair spinal cord injury and its preparation method and application
  • A kind of hierarchical porous functional scaffold material for mobilizing endogenous neural stem cells to repair spinal cord injury and its preparation method and application
  • A kind of hierarchical porous functional scaffold material for mobilizing endogenous neural stem cells to repair spinal cord injury and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Preparation of the multi-level porous functional scaffold material of the present invention (1)

[0052] 1. Chitosan grafted double bond:

[0053] Weigh 2.0 g of chitosan into a three-necked flask, add 150 ml of 2% acetic acid aqueous solution, and dropwise add a small amount of KOH aqueous solution to adjust the pH to 3.8. Then, 3.51 g of glycidyl methacrylate (GMA) was slowly added dropwise, continuously stirred, and reacted at 60° C. for 6 hours. Then pour the reaction mixture into acetone to obtain a large amount of flocculent precipitate, then pour the flocculent into acetonitrile to obtain a powdery precipitate, and finally wash with acetone several times and dissolve it in water, then put it into a dialysis bag with a molecular weight of 3500 Dialyzed for 3 days and freeze-dried to obtain the raw material Chitosan-GMA, which was set aside. The chitosan grafted with double bonds was characterized by infrared spectroscopy and H-NMR.

[0054] result: f...

Embodiment 2

[0066] Example 2 Preparation of the multi-level porous functional scaffold material of the present invention (2)

[0067] 1. Chitosan grafted double bond:

[0068] With embodiment 1.

[0069] 2. Preparation of materials:

[0070] Weigh 50mg Chitosan-GMA into a small glass bottle, then add 25μL BIS (N,N'-methylenebisacrylamide, 10mg / mL) crosslinking agent (0.5% of the monomer), then add 2mg photoinitiator 2-Hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone (4% of the monomer), and finally add 1700 μL H 2 O, stirred at 37°C for 2 hours to dissolve it to form a precursor, that is, a gel-forming precursor.

[0071] After the dissolution is complete, the gel-forming precursor solution is centrifuged at 4500r / min for 4min to remove air bubbles. The solution is then injected into the capillary using an ear wash bulb.

[0072] The capillary injected with the solution was placed in liquid nitrogen at a constant speed (2.5 mm / s) using a lifter for freezing guidance. Immediately a...

Embodiment 3

[0073] Example 3 Preparation of multi-level porous functional scaffold material of the present invention (3)

[0074] 1. Chitosan grafted double bond:

[0075] With embodiment 1.

[0076] 2. Preparation of materials:

[0077] Weigh 50mg Chitosan-GMA into a small glass bottle, then add 50μL BIS (N,N'-methylenebisacrylamide, 10mg / mL) crosslinking agent (1% of the monomer), then add 1mg photoinitiator 2-Hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone (2% of the monomer), and finally add 2200 μL H 2 O, stirred at 37°C for 2 hours to dissolve it to form a precursor, that is, a gel-forming precursor.

[0078] After the dissolution is complete, the gel-forming precursor solution is centrifuged at 4500r / min for 4min to remove air bubbles. The solution is then injected into the capillary using an ear wash bulb.

[0079] Place the capillary injected with the solution in liquid nitrogen at a constant speed (3.5mm / s) for freezing guidance. Immediately after the guidance, the capi...

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Abstract

The invention relates to a multi-level porous functional scaffold material for mobilizing endogenous neural stem cells to repair spinal cord injury and its preparation method and application. The material is prepared by the following method: chitosan modified with double bonds and cross-linking agent Mix BIS, 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone and distilled water to obtain a precursor solution, add or not add growth factors to prepare a gel precursor solution, and place it in liquid nitrogen for ice crystal guidance , and then UV light at -20°C initiates gelation. The material of the present invention has guide holes of different sizes along the long axis of the material, which provides the space structure required for the reconstruction of the neural circuit, and facilitates the directional migration of endogenous neural stem cells to the implanted area of ​​the material and the formation of new neurons. The designed multi-level structure grows to form a neural network, reconstructs the neural circuit in the damaged area, and promotes the recovery of motor function after spinal cord injury.

Description

technical field [0001] The invention relates to the technical field of nerve regeneration and repair, in particular to a multi-level porous functional scaffold material for mobilizing endogenous neural stem cells to repair spinal cord injuries, a preparation method and application thereof. Background technique [0002] Spinal cord injury (SCI) usually refers to the direct injury of the spinal cord caused by various reasons such as trauma, resulting in the loss of motor and sensory functions below the level of injury to varying degrees, and even complete loss of motor and sensory functions leading to paraplegia. SCI has a high morbidity rate. In addition to causing quadriplegia and hemiplegia, it may also produce other secondary diseases, which seriously affect the quality of life of patients. The treatment and rehabilitation of SCI has always been one of the major problems in the field of basic and clinical medicine. [0003] Spinal cord injury often leads to continuous los...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/56A61L27/54A61L27/26A61L27/36C08F251/00C08F222/38C08F2/48
CPCA61L27/26A61L27/3675A61L27/54A61L27/56A61L2300/602A61L2430/00C08F2/48C08F251/00C08L5/08C08L51/02C08F222/385
Inventor 程黎明王启刚程和丽徐委
Owner SHANGHAI TONGJI HOSPITAL
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