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A kind of preparation method of sensing electrode based on graphene oxide sucrose biosensor

A biosensor and sensing electrode technology, applied in the field of biosensors, can solve the problems of high sucrose concentration, cumbersome steps, and difficulty in sucrose detection

Active Publication Date: 2021-07-09
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This detection method has various shortcomings such as cumbersome steps, long pretreatment cycle, large hysteresis and interference, and high cost. However, the emerging enzyme biosensor uses sucrase, mutarotase and glucose oxidase as immobilized enzyme components, and has real-time Convenience, high sensitivity, strong specificity, etc.
At the same time, the concentration of sucrose used in industrial production is relatively high, and the detection of sucrose is more difficult

Method used

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  • A kind of preparation method of sensing electrode based on graphene oxide sucrose biosensor
  • A kind of preparation method of sensing electrode based on graphene oxide sucrose biosensor
  • A kind of preparation method of sensing electrode based on graphene oxide sucrose biosensor

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preparation example Construction

[0031] In one embodiment, a kind of sensing electrode preparation method based on graphene oxide sucrose biosensor comprises the steps:

[0032] 1. Preparation of hybrid nanosheets;

[0033] II. Preparation of mirror glassy carbon electrode;

[0034] III. Post-processing of specular glassy carbon electrodes.

[0035] In one embodiment, the preparation process of the hybrid nanosheets includes dissolving graphene oxide and graphene quantum dots in water, ultrasonication, reaction, centrifugation, and drying.

[0036] Preferably, the preparation process of the hybrid nanosheets is to dissolve graphene oxide and graphene quantum dots in ultrapure water, and sonicate for 1 to 5 hours; then react the resulting solution at 150 to 200°C for 10 to 20 hours; The obtained solution is centrifuged at 6000-10000 rpm for 3-9 hours; then dried in a vacuum oven at 45-75° C. for 10-20 hours.

[0037] More preferably, the preparation process of the hybrid nanosheets is to dissolve graphene o...

Embodiment 1

[0090] Embodiment 1 of the present invention provides a kind of sensing electrode based on graphene oxide sucrose biosensor, and its preparation method is as follows:

[0091] I. Preparation of hybrid nanosheets: Dissolve graphene oxide and graphene quantum dots in ultrapure water at a concentration of 1.3 mg / mL, ultrasonicate for 2 hours; then react the resulting solution at 180°C for 14 hours; The solution was centrifuged at 8000rpm for 6h; then dried in a vacuum oven at 60°C for 14h;

[0092] The particle size of graphene quantum dots is 7nm, and the sheet diameter of graphene oxide is 220nm; the weight ratio of graphene oxide to graphene quantum dots is 1:1;

[0093] II. Preparation of mirror glassy carbon electrode:

[0094] (1) polishing the glassy carbon electrode with alumina powder;

[0095] (2) The material obtained in step (1) was ultrasonically treated with 50% (v / v) nitric acid solution, absolute ethanol and ultrapure water of the same volume for 2 hours;

[00...

Embodiment 2

[0110] Embodiment 2 of the present invention provides a kind of sensing electrode based on graphene oxide sucrose biosensor, and its preparation method is as follows:

[0111] I. Preparation of hybrid nanosheets: Dissolve graphene oxide and graphene quantum dots in ultrapure water at a concentration of 2 mg / mL, ultrasonicate for 5 hours; then react the resulting solution at 200°C for 20 hours; Centrifuge at 10,000rpm for 9h; then dry it in a vacuum oven at 75°C for 20h;

[0112] The particle size of graphene quantum dots is 7nm, and the sheet diameter of graphene oxide is 220nm; the weight ratio of graphene oxide to graphene quantum dots is 1:1.5;

[0113] II. Preparation of mirror glassy carbon electrode:

[0114] (1) polishing the glassy carbon electrode with alumina powder;

[0115] (2) The material obtained in step (1) was ultrasonically treated with 50% (v / v) nitric acid solution, absolute ethanol and ultrapure water of the same volume for 5 hours;

[0116] (3) drying ...

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Abstract

The invention relates to the technical field of biosensors, and more specifically, the invention provides a method for preparing a sensing electrode based on a graphene oxide sucrose biosensor. The first party of the present invention provides a method for preparing sensing electrodes based on graphene oxide sucrose biosensors, including the preparation of hybrid nanosheets, the preparation of mirror glassy carbon electrodes, and the post-treatment of mirror glassy carbon electrodes; wherein, hybridization The raw materials for the preparation of nanosheets include graphene oxide and graphene quantum dots; at the same time, chitosan is used to immobilize sucrase, mutarotase and glucose oxidase on the electrode surface modified by graphene oxide. The sucrose biosensing electrode prepared by this method and the sensor containing the electrode have a high linear detection limit, which makes up for the defect that high-concentration sucrose cannot be directly detected in fermentation production, and also lays a theoretical and application foundation for the subsequent development of sucrose on-line detection equipment.

Description

technical field [0001] The invention relates to the technical field of biosensors, and more specifically, the invention provides a method for preparing a sensing electrode based on a graphene oxide sucrose biosensor. Background technique [0002] Sucrose is formed by condensing and dehydrating the hemiacetal hydroxyl group of a molecule of glucose and the hemiacetal hydroxyl group of a molecule of fructose. Sucrose is almost ubiquitously found in leaves, flowers, stems, seeds and fruits of the plant kingdom, and is particularly abundant in sugar cane, sugar beet and maple sap. Sucrose is the raw material of various products, and it has a wide range of applications in industry and food. It can be used as a nutrient for yeast and a food sweetener. [0003] At present, most of the research on sucrose focuses on the efficient production and later utilization of sucrose, and less attention is paid to the change of sucrose concentration in the production process. Moreover, most ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/00C12Q1/48C12Q1/34G01N27/30G01N27/327G01N27/48
CPCC12Q1/005C12Q1/34C12Q1/48G01N27/308G01N27/3277G01N27/3278G01N27/48
Inventor 周杰庄妮莎董维亮薛瑞姜岷
Owner NANJING TECH UNIV