Method for screening radix peucedani component in lung ventilating and regulating preparation by adopting HPLC (High Performance Liquid Chromatography) method

A Xuanlifei Pill and screening technology, which is applied in the field of natural product testing, can solve problems affecting the quality of preparations, achieve the effects of ensuring clinical efficacy, reducing testing costs and time, and improving work efficiency

Inactive Publication Date: 2019-12-13
湖南食品药品职业学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection method of Qianhu is not involved in the current standard of Tongxuanlifei preparation. Qianhu is the subject drug of Tongxuanlifei preparation, and the feeding problem will definitely affect the quality of the preparation.

Method used

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  • Method for screening radix peucedani component in lung ventilating and regulating preparation by adopting HPLC (High Performance Liquid Chromatography) method
  • Method for screening radix peucedani component in lung ventilating and regulating preparation by adopting HPLC (High Performance Liquid Chromatography) method
  • Method for screening radix peucedani component in lung ventilating and regulating preparation by adopting HPLC (High Performance Liquid Chromatography) method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment one: the establishment of detection method

[0034] 1. Instruments, reagents and materials

[0035] Instruments: Waters2695e high performance liquid chromatography, chromatographic workstation data processing system; Sartorius BP211D electronic analytical balance; ultrapure water system (MILLIPORE); ultrasonic cleaner (power 500W, frequency 40kHz).

[0036] Reagents: methanol is chromatographically pure (product of Fisher Scientific, USA); methanol is analytically pure (Sinopharm Chemical Reagent Co., Ltd.); water is ultrapure water; neutral alumina is used for chromatography (Chengdu Kelong Chemical Co., Ltd.) .

[0037] Materials: Prostinidin reference substance (batch number: 111821-201604, purity: 99.6%, for content determination), cantiflorin (for content determination, lot number: 111711-201703), cantiflorin (for content determination, Batch number: 111904-201804, 98.9%), the above reference substances were all purchased from China National Institutes...

Embodiment 2

[0064] Example 2, Tongxuanlifei pill sample detection

[0065] 1. The preparation and chromatographic conditions of the mixed reference solution are the same as above.

[0066] 2. Take Tongxuanli Fei Pills with batch number 20180512, and the sample preparation is the same as above.

[0067] 3. HPLC determination: Accurately draw 5 μl each of the mixed reference substance solution and the test solution, inject it into a high-performance liquid chromatograph, and measure it. According to the peak areas of prosperidin, proscurdin A and prosperidin B, the external standard method is adopted According to the calculated content, the contents of proscurdinin, prostinidin and prostinidin are 0.133mg / g, 0.324mg / g, and 0.128mg / g, respectively. Since the three characteristic components were detected at the same time in the HPLC chromatogram, it indicated that this batch of Tongxuanlifei Pills was fed with Zihua Qianhu, which may be a single feeding of Zihua Qianhu, or it may be a non-si...

Embodiment 3

[0068] Example three, Tongxuanli lung tablet sample detection

[0069] 1. The preparation and chromatographic conditions of the mixed reference solution are the same as above.

[0070] 2. Take Tongxuanli lung tablets with batch number 181001, and the sample preparation is the same as above.

[0071] 3. HPLC determination: Accurately draw 5 μl each of the mixed reference substance solution and the test solution, inject it into a high performance liquid chromatograph, measure, and do not detect prosarcoside, and use The content was calculated by the external standard method, and the contents of proscurdin and proscurdin were 0.321mg / g and 0.108mg / g, respectively. As the HPLC chromatograms detected the prosaldin and prosperidin, it indicated that this batch of Tongxuanlifei Tablets was fed with succulent, which may be a single or non-single variety of succulent.

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Abstract

The invention relates to a method for screening a radix peucedani component in a lung ventilating and regulating preparation by adopting an HPLC (High Performance Liquid Chromatography) method. According to the method, the high performance liquid chromatography (HPLC) method is adopted to simultaneously measure the contents of the characteristic components of the radix peucedani and angelica decursiva (Miq.) Franch. et Sav. with a mixed contrast solution used as reference, and therefore, technical support for testing whether the radix peucedani is used for replacing the angelica decursiva (Miq.) Franch. et Sav. in the lung ventilating and regulating preparation is provided, and the quality standard of the lung ventilating and regulating preparation is improved. The method is simple, convenient and rapid to operate. With the method adopted, detection cost and time are greatly reduced; working efficiency is improved; the quality of the lung ventilating and regulating preparation can be effectively comprehensively and accurately controlled; the stability and uniformity of the quality of the product are ensured; and the safety and effectiveness of the taking of the product is achieved.

Description

[0001] Technical field [0002] The invention relates to a method for screening Radix Peucedanum in Tongxuanlifei preparation, which belongs to the technical field of natural product detection. Background technique [0003] Peucedanum has the effects of reducing qi and reducing phlegm, dispersing wind and clearing heat, and is clinically used for diseases such as wind-heat cough, asthma with excessive phlegm, and yellow and thick phlegm. It is widely used in Chinese patent medicines such as clearing the lungs, relieving cough and reducing phlegm, and colds, and has a large market demand. The 2000 edition of "Chinese Pharmacopoeia" and before the use of Baihua Qianhu and Zihua Qianhu as genuine products, "Chinese Pharmacopoeia" 2005 edition only Baihua Qianhu was included in the Pharmacopoeia as the genuine product, and Zihua Qianhu was deleted. "Chinese Pharmacopoeia" Since the 2010 edition, Zihua Qianhu has been listed as an independent variety. The 2015 edition of the "Chi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 姚蓉罗疆南于勇
Owner 湖南食品药品职业学院
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