A kind of preparation method of clostridial phage protein
A technology of bacterial protein and Clostridium, which is applied in the field of bacterial protein preparation, can solve problems such as inability to concentrate by centrifugation, many fragments, and poor quality
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0031] The embodiment of the present invention provides a method for preparing Clostridium phage protein, figure 1 It is a process step diagram of an embodiment of a method for preparing a clostridial protein, combining figure 1 , this method includes:
[0032] S1, preparing the first flocculant mother liquor and preparing the second flocculant mother liquor. The flocculants used to configure the first and second flocculant mother liquors are one or more of polyacrylamide, chitosan, sodium carboxymethylcellulose, polyaluminum sulfate, and sodium polyacrylate. When preparing, the flocculant should be slowly sprinkled into the water. Sprinkling too much at one time will cause the flocculant to agglomerate and dissolve insufficiently, resulting in waste of flocculant and affecting the quality of Clostridia protein; the water should be neutral and not Contains salt or impurities, this is because charged ions such as calcium and magnesium in the water will combine with the floccu...
Embodiment 1
[0052] In this example, industrial gas was used as raw material, Clostridium ethanolum was used as fermentation bacteria to prepare industrial mash obtained from the gas ethanol process as raw material, polyacrylamide was used as flocculant to prepare the first flocculant mother liquor, polyacrylamide was used as The flocculant is used to prepare the second flocculant mother liquor. The preparation temperature of the first flocculant mother liquor and the second flocculant mother liquor are both controlled at 50°C. The volume of the first flocculant mother liquor is 3% of the mash volume, and the primary flocculation temperature is controlled at 42 ℃; the volume of the second flocculant mother liquor is 3% of the air flotation scum, and the secondary flocculation temperature is controlled at 38°C to obtain a mixed solution; the mixed solution overflows into the screw stacker and is dehydrated and concentrated to obtain the first concentrated slag. The first concentrated slag is...
Embodiment 2
[0055] As another embodiment of this description, different from Example 1, the preparation temperature of the first flocculant mother liquor and the second flocculant mother liquor are both controlled at 35°C, and the volume of the first flocculant mother liquor is 4% of the mash , the primary flocculation temperature is controlled at 50°C, the volume of the second flocculant mother liquor is 4% of the air-float scum, the volume of the second flocculant mother liquor is 3% of the air-float scum, and the secondary flocculation temperature is controlled at 50°C, The water content of the second concentrated slag is 50%.
[0056] It is worth noting that the crude protein content of the Clostridium ethanolum thalline protein obtained in this example is 85%, the moisture content is 10.1%, and the ash content is 5.2%.
PUM
| Property | Measurement | Unit |
|---|---|---|
| water content | aaaaa | aaaaa |
| relative humidity | aaaaa | aaaaa |
| Ash content | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More