Surface-enhanced Raman flexible substrate for quantitative detection of pesticide parathion-methyl and detection method

Abstract

The invention discloses a surface-enhanced Raman flexible substrate and a method for quantitatively detecting a pesticide parathion-methyl. A filter paper sheet is soaked in a gold nanoparticle sol for a certain time, Au NP is adsorbed to the surface of the filter paper sheet to form the surface-enhanced Raman flexible substrate, and a strong Raman signal is generated, and residual pesticide molecules are detected; the reproducibility, stability and sensitivity of the substrate are evaluated by using a probe molecule 4-mercaptobenzoic acid, and a linear relationship between the concentrationof 4-MBA and Raman characteristic peak intensity is established; a parathion-methyl standard solution is detected by using the paper surface enhanced Raman flexible substrate, and a corresponding standard curve is established; and the substrate is applied to detection of pesticide residues on the surface of an actual sample through a pasting-uncovering method, and a result shows that the recoveryrate is high. According to the invention, the surface-enhanced Raman flexible substrate is constructed by combining a nano material with paper, and is used for rapidly detecting pesticide residues onthe surface of the actual sample, so that complex sample pretreatment is omitted, and the method has the characteristics of simplicity and high efficiency.

Description

technical field [0001] The invention relates to the field of analysis science and technology, in particular to a surface-enhanced Raman flexible substrate and a detection method for the quantitative detection of the pesticide methyl parathion. Background technique [0002] With the increase of social population and the development of agricultural production, organophosphorus pesticides (OP) are widely used in the production process. Parathion, endogenous phosphorus, malathion, dimethoate, trichlorfon, etc. are common organophosphorus pesticides used to control plant diseases and insect pests, but these pesticides tend to remain on the surface of fruit and vegetable crops and in the soil, and pass through The enrichment and amplification effect of the food chain accumulates in the human body, and after entering the human body, it is easy to interact with acetylcholine enzyme, resulting in a large accumulation of acetylcholine, destroying the central nervous system, and a seri...

AI Technical Summary

Problems solved by technology

The signal enhancement effect of SERS is related to the properties of the substrate. At present, a variety of rigid substrates have been developed for the food safety detection industry, such as silicon wafers, glass sheets, electrodes, etc. These materials have the advantage of not being easy to bend, but they are also easy to break and are not easy to Complete contact with the sample surface, it is difficult to collect the sample effectively

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